ID   RSH2L_ARATH             Reviewed;         710 AA.
AC   Q9M5P6;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Probable GTP diphosphokinase RSH2, chloroplastic;
DE            EC=2.7.6.5;
DE   AltName: Full=RelA/SpoT homolog 2;
DE            Short=AtRSH2;
DE   AltName: Full=ppGpp synthetase RSH2;
DE   Flags: Precursor;
GN   Name=RSH2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=10725385; DOI=10.1073/pnas.97.7.3747;
RA   van der Biezen E.A., Sun J., Coleman M.J., Bibb M.J., Jones J.D.;
RT   "Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:3747-3752(2000).
CC   -!- FUNCTION: Probable ppGpp (guanosine 3'-diphosphate 5'-diphosphate)
CC       synthetase that may be involved in a rapid plant ppGpp-mediated
CC       response to pathogens and other stresses. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate;
CC         Xref=Rhea:RHEA:22088, ChEBI:CHEBI:30616, ChEBI:CHEBI:37565,
CC         ChEBI:CHEBI:142410, ChEBI:CHEBI:456215; EC=2.7.6.5;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the RelA/SpoT family. {ECO:0000305}.
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DR   EMBL; AF225703; AAF37282.1; -; mRNA.
DR   AlphaFoldDB; Q9M5P6; -.
DR   SMR; Q9M5P6; -.
DR   ExpressionAtlas; Q9M5P6; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008728; F:GTP diphosphokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015969; P:guanosine tetraphosphate metabolic process; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd00077; HDc; 1.
DR   CDD; cd05399; NT_Rel-Spo_like; 1.
DR   Gene3D; 3.30.460.10; -; 1.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   InterPro; IPR043519; NT_sf.
DR   InterPro; IPR007685; RelA_SpoT.
DR   Pfam; PF04607; RelA_SpoT; 1.
DR   SMART; SM00471; HDc; 1.
DR   SMART; SM00954; RelA_SpoT; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
DR   PROSITE; PS51831; HD; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chloroplast; GTP-binding; Kinase; Nucleotide-binding; Plastid;
KW   Stress response; Transferase; Transit peptide.
FT   TRANSIT         1..63
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           64..710
FT                   /note="Probable GTP diphosphokinase RSH2, chloroplastic"
FT                   /id="PRO_0000429848"
FT   DOMAIN          233..337
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
SQ   SEQUENCE   710 AA;  79207 MW;  7638AC41EDE0DDB4 CRC64;
     MVVATTIALY ASPPSSVCST PHQISCDLDL TSRSSSTSSS MASSPQKPIV GGLSSLFSSA
     SVKSSSSSSC SYSTAVDEFS SLRYDRSDDL KDLSFSSSFG YSPAKFVNSF RRDHQSPISV
     LHGPVSCSCS PPMRMSRDRN LDGSFRLGAS RLFNGFVRKA LGSCVDYELG SDSGLVDELT
     FPMEVDTIKP YARDLLRRAQ LRHKIFNDES VIKAFYEAEK AHRGQMRASR DPYLQHCVET
     AMLLANIGAN STVVVAGLLH DTVDDSFMSY DYILRNFGAG VADLVEGVSK LSQLSKLARE
     NNTACKTVEA DRLHPMFLAM ADARAVLIKL ADRLHNMKTL YALSPVKQQR FAKETLEIFA
     PLANCLGIST WKVQLENLCF KHLYPNQHNE MSTMLEDSFD EAMITSAIEK LDQALKKAGI
     SYHVLCGRHK SLYSIYSKML KKKLTVDEIH DIHGLRLIVD NEGDCYKALG VVHSLWSEVP
     GKLKDYITHP KFNGYQSLHT VVMDNGTVPL EVQIRTQEMH LQAEFGFAAH WRYKEGGCKY
     SSFVLQMVEW ARWVVTWHCE AMSKDRSSIS SSDSIKPPLQ VFRLTLEDCP ASYKPNSSQD
     GPVYVIVIEN DKMSVQEFPA SSTVSDLLSR AGPGSSRWSM YGIPAKEELR PRLNQIPVSD
     LKWKLKMGDV VELTPKIPDE SLTEYREEIQ RMYDRGLAFS RPGTMVGWGS