RSH2_ORYSJ
ID RSH2_ORYSJ Reviewed; 718 AA.
AC Q7XAP4; A0A0P0XGI7;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Probable GTP diphosphokinase RSH2, chloroplastic;
DE EC=2.7.6.5;
DE AltName: Full=RelA/SpoT homolog 2;
DE Short=OsRSH2;
DE AltName: Full=ppGpp synthetase RSH2;
DE Flags: Precursor;
GN Name=RSH2; OrderedLocusNames=Os08g0457600, LOC_Os08g35620;
GN ORFNames=OsJ_27564, P0493A04.9;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RA Tozawa Y.;
RT "Oryza sativa (japonica cultivar-group) plastid (p)ppGpp synthase RSH2.";
RL Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Probable ppGpp (guanosine 3'-diphosphate 5'-diphosphate)
CC synthetase that may be involved in a rapid plant ppGpp-mediated
CC response to pathogens and other stresses. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate;
CC Xref=Rhea:RHEA:22088, ChEBI:CHEBI:30616, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:142410, ChEBI:CHEBI:456215; EC=2.7.6.5;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RelA/SpoT family. {ECO:0000305}.
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DR EMBL; AB095096; BAC81140.1; -; mRNA.
DR EMBL; AP004586; BAD09583.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23883.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT05740.1; -; Genomic_DNA.
DR EMBL; CM000145; EEE68808.1; -; Genomic_DNA.
DR EMBL; AK065020; BAG89329.1; -; mRNA.
DR EMBL; AK099624; BAG94226.1; -; mRNA.
DR RefSeq; XP_015650642.1; XM_015795156.1.
DR AlphaFoldDB; Q7XAP4; -.
DR SMR; Q7XAP4; -.
DR STRING; 4530.OS08T0457600-01; -.
DR PaxDb; Q7XAP4; -.
DR PRIDE; Q7XAP4; -.
DR EnsemblPlants; Os08t0457600-01; Os08t0457600-01; Os08g0457600.
DR EnsemblPlants; Os08t0457600-02; Os08t0457600-02; Os08g0457600.
DR GeneID; 4345756; -.
DR Gramene; Os08t0457600-01; Os08t0457600-01; Os08g0457600.
DR Gramene; Os08t0457600-02; Os08t0457600-02; Os08g0457600.
DR KEGG; osa:4345756; -.
DR eggNOG; KOG1157; Eukaryota.
DR HOGENOM; CLU_012300_7_1_1; -.
DR InParanoid; Q7XAP4; -.
DR OMA; PNCKLRM; -.
DR OrthoDB; 505873at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q7XAP4; OS.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0008728; F:GTP diphosphokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0015969; P:guanosine tetraphosphate metabolic process; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00077; HDc; 1.
DR CDD; cd05399; NT_Rel-Spo_like; 1.
DR Gene3D; 3.30.460.10; -; 1.
DR InterPro; IPR003607; HD/PDEase_dom.
DR InterPro; IPR006674; HD_domain.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR007685; RelA_SpoT.
DR Pfam; PF04607; RelA_SpoT; 1.
DR SMART; SM00471; HDc; 1.
DR SMART; SM00954; RelA_SpoT; 1.
DR SUPFAM; SSF81301; SSF81301; 1.
DR PROSITE; PS51831; HD; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; GTP-binding; Kinase; Nucleotide-binding; Plastid;
KW Reference proteome; Stress response; Transferase; Transit peptide.
FT TRANSIT 1..68
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 69..718
FT /note="Probable GTP diphosphokinase RSH2, chloroplastic"
FT /id="PRO_0000429849"
FT DOMAIN 243..347
FT /note="HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
FT REGION 9..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 718 AA; 79148 MW; C618C46D87617830 CRC64;
MSVPAIAVYT SPPGAVYTSS SSSELEASSR GSAPCATAAP PSPASSHRHQ AIAGGLSCLF
SSPTAAPRAA AAQDELGALW HDRSGEATAV GGGGGGGGYS YPQPSSPFKW RDMLHHSPVP
LFHSPASSPA SRSPSASWLA GRERERLFSS FVRNALGSCV DYAPVAALPL GVSAAVGVGA
GELAFELDEH LSEAEPSCEP YARDLLAGAQ ARHRIFHDEL VVKAFFEAER AHRGQTRASG
DPYLQHCVET AVLLAKIGAN ATVVSAGLLH DTIDDSFMDY DQIFRMFGAG VADLVEGVSK
LSHLSKLARD NNTASRTVEA DRLHTMFLAM ADARAVLIKL ADRLHNMKTI EALPLVKQQR
FAKETMEIFV PLANRLGIAS WKDQLENICF KHLNPEEHKE LSSKLVISFD EALLTSTLDK
LDKGLRDEGI SYHSLSGRHK SLYSIYSKMI KKNLTMDDVH DIHGLRLVVD TEQDCYQALD
IVHKLWPRVA GRFKDYILHP KLNGYRSLHT VIMCEGIHPF EVQIRTKEMH LQAEYGFAAH
WRYKEGGCKH SFVLQMVEWA RWVLTWQCEA MSKERSSGLG RSDAIRPPCP FPSHSEDCPY
SYTRQCNHDG PIFVIMLEHD KMSVQELPAN STVVDLMERV GANSPRCSPY SFPLKEELRP
RVNHKPISDP NRKLCMGDVV ELTPALPHKS LTEYREEIQR MYERGGFALA TTRDGPAS
