RSH3_ORYSJ
ID RSH3_ORYSJ Reviewed; 708 AA.
AC Q67UU0; Q0J1G0; Q7XAP3;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Probable GTP diphosphokinase RSH3, chloroplastic;
DE EC=2.7.6.5;
DE AltName: Full=RelA/SpoT homolog 3;
DE Short=OsRSH3;
DE AltName: Full=ppGpp synthetase RSH3;
DE Flags: Precursor;
GN Name=RSH3; OrderedLocusNames=Os09g0442600, LOC_Os09g27050;
GN ORFNames=P0046G12.25;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RA Tozawa Y.;
RT "Oryza sativa (japonica cultivar group) mRNA for plastid (p)ppGpp synthase
RT RSH3.";
RL Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP INDUCTION.
RX PubMed=14660585; DOI=10.1074/jbc.m311573200;
RA Givens R.M., Lin M.H., Taylor D.J., Mechold U., Berry J.O., Hernandez V.J.;
RT "Inducible expression, enzymatic activity, and origin of higher plant
RT homologues of bacterial RelA/SpoT stress proteins in Nicotiana tabacum.";
RL J. Biol. Chem. 279:7495-7504(2004).
CC -!- FUNCTION: Probable ppGpp (guanosine 3'-diphosphate 5'-diphosphate)
CC synthetase that may be involved in a rapid plant ppGpp-mediated
CC response to pathogens and other stresses. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate;
CC Xref=Rhea:RHEA:22088, ChEBI:CHEBI:30616, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:142410, ChEBI:CHEBI:456215; EC=2.7.6.5;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- INDUCTION: By jasmonic acid (JA) and infection with the fungal pathogen
CC Magnaporthe grisea. {ECO:0000269|PubMed:14660585}.
CC -!- SIMILARITY: Belongs to the RelA/SpoT family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC81141.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=BAF25205.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB095097; BAC81141.1; ALT_SEQ; mRNA.
DR EMBL; AP005419; BAD38079.1; -; Genomic_DNA.
DR EMBL; AP008215; BAF25205.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014965; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015611622.1; XM_015756136.1.
DR AlphaFoldDB; Q67UU0; -.
DR SMR; Q67UU0; -.
DR STRING; 4530.OS09T0442600-01; -.
DR PaxDb; Q67UU0; -.
DR PRIDE; Q67UU0; -.
DR GeneID; 4347182; -.
DR KEGG; osa:4347182; -.
DR eggNOG; KOG1157; Eukaryota.
DR HOGENOM; CLU_012300_7_1_1; -.
DR InParanoid; Q67UU0; -.
DR OrthoDB; 505873at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR Genevisible; Q67UU0; OS.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0008728; F:GTP diphosphokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0015969; P:guanosine tetraphosphate metabolic process; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00077; HDc; 1.
DR CDD; cd05399; NT_Rel-Spo_like; 1.
DR Gene3D; 3.30.460.10; -; 1.
DR InterPro; IPR003607; HD/PDEase_dom.
DR InterPro; IPR006674; HD_domain.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR007685; RelA_SpoT.
DR Pfam; PF04607; RelA_SpoT; 1.
DR SMART; SM00471; HDc; 1.
DR SMART; SM00954; RelA_SpoT; 1.
DR SUPFAM; SSF81301; SSF81301; 1.
DR PROSITE; PS51831; HD; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; GTP-binding; Kinase; Nucleotide-binding; Plastid;
KW Reference proteome; Stress response; Transferase; Transit peptide.
FT TRANSIT 1..58
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 59..708
FT /note="Probable GTP diphosphokinase RSH3, chloroplastic"
FT /id="PRO_0000429852"
FT DOMAIN 233..337
FT /note="HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 109..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..127
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 708 AA; 78925 MW; 6E57A1FD3EE8F4F1 CRC64;
MSLPAISLYT SPPPGAVYSS EFDPSSRGSS PPCSTAPPST SHRPPAAAGG LSCLFSSPAA
AASPPRAPPH DELGALWQDR SDEPAFAGGG GGYSSSPLKW RDLHHHHHHS PVSVFQGPSS
SPAASRSPPA SWLAGRDRDR ERLFAGFVRN ALGSCVDYAP ALSPRSEVGG GELAFELDEN
LAEASPACEP CARELLAGAQ ARHRIFHEEL VVKTFFEAEK AHRGQTRASG DPYLQHCVET
AVLLANIGAN STVVSAGLLH DTIDDSFIDY DHIFHMFGAG VADLVEGVSK LSHLSKLARD
NNTASRIVEA DRLHTMLLAM ADARAVLIKL ADRVHNMKTL EALPLGKQQR FAKETMEIFV
PLANRLGIAS WKDQLENLCF KHLNPEEHKD LSSKLTKSFD EVLITSAVDK LDRGLRDAGL
SYHNLSGRHK SLYSIHNKML KKNLTMDEIH DIHGLRLVFE KEEDCYRALD VVHELWPQVP
GRFKDYISRP KLNGYRSLHT VVMSENVHPF EVQIRTKEMH LQAEYGFAAH WRYKEGTCRH
SFVLQMVEWA RWVLTWQCEA MNKERPASLG DSDAIRPPCP FPMHSEDCPY SYTRQCDHDG
PIFVILLEHD KMSVQEFQAN STVMNLMDRV GTNTPRWSPY RIPMKEDLRP KVNHEPISDL
NRKLSMGDVV ELTPALPHES LPNYREEIQR MYDRGGFALA TRGGSSRR
