RSH4A_MOUSE
ID RSH4A_MOUSE Reviewed; 716 AA.
AC Q8BYM7; B2RQN0;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Radial spoke head protein 4 homolog A;
DE AltName: Full=Radial spoke head-like protein 3;
GN Name=Rsph4a; Synonyms=Rshl3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 111-716.
RC STRAIN=C57BL/6J; TISSUE=Hypothalamus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-395, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP INTERACTION WITH RSPH6A.
RX PubMed=30185526; DOI=10.1242/jcs.221648;
RA Abbasi F., Miyata H., Shimada K., Morohoshi A., Nozawa K., Matsumura T.,
RA Xu Z., Pratiwi P., Ikawa M.;
RT "RSPH6A is required for sperm flagellum formation and male fertility in
RT mice.";
RL J. Cell Sci. 131:0-0(2018).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=30239614; DOI=10.1093/biolre/ioy202;
RA Paudel B., Gervasi M.G., Porambo J., Caraballo D.A., Tourzani D.A.,
RA Mager J., Platt M.D., Salicioni A.M., Visconti P.E.;
RT "Sperm capacitation is associated with phosphorylation of the testis-
RT specific radial spoke protein Rsph6.";
RL Biol. Reprod. 100:440-454(2019).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=32203505; DOI=10.1371/journal.pgen.1008664;
RA Yoke H., Ueno H., Narita A., Sakai T., Horiuchi K., Shingyoji C.,
RA Hamada H., Shinohara K.;
RT "Rsph4a is essential for the triplet radial spoke head assembly of the
RT mouse motile cilia.";
RL PLoS Genet. 16:e1008664-e1008664(2020).
CC -!- FUNCTION: Component of the axonemal radial spoke head which plays an
CC important role in ciliary motility (PubMed:32203505). Essential for
CC triplet radial spokes (RS1, RS2 and RS3) head assembly in the motile
CC cilia (PubMed:32203505). {ECO:0000269|PubMed:32203505}.
CC -!- SUBUNIT: Interacts with RSPH6A. {ECO:0000269|PubMed:30185526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000305}. Cell projection, cilium {ECO:0000269|PubMed:32203505}.
CC Note=Radial spoke. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the trachea, ependymal cells and
CC oviduct (at protein level) (PubMed:32203505, PubMed:30239614).
CC Expressed in brain, kidney and testis. {ECO:0000269|PubMed:30239614,
CC ECO:0000269|PubMed:32203505}.
CC -!- DISRUPTION PHENOTYPE: Mice show hydrocephalus and the tracheal and
CC ependymal cell cilia show clockwise rotation motion instead of planar
CC beating (PubMed:32203505). All three types of radial spoke heads (RS1,
CC RS2 and RS3) are missing in the tracheal cilia (PubMed:32203505).
CC {ECO:0000269|PubMed:32203505}.
CC -!- SIMILARITY: Belongs to the flagellar radial spoke RSP4/6 family.
CC {ECO:0000305}.
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DR EMBL; AC153961; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC138000; AAI38001.1; -; mRNA.
DR EMBL; BC138001; AAI38002.1; -; mRNA.
DR EMBL; AK038980; BAC30192.1; -; mRNA.
DR CCDS; CCDS48534.1; -.
DR RefSeq; NP_001156429.1; NM_001162957.1.
DR PDB; 7DMP; EM; 3.20 A; B/b=1-716.
DR PDBsum; 7DMP; -.
DR AlphaFoldDB; Q8BYM7; -.
DR SMR; Q8BYM7; -.
DR STRING; 10090.ENSMUSP00000131647; -.
DR iPTMnet; Q8BYM7; -.
DR PhosphoSitePlus; Q8BYM7; -.
DR MaxQB; Q8BYM7; -.
DR PaxDb; Q8BYM7; -.
DR PRIDE; Q8BYM7; -.
DR ProteomicsDB; 256783; -.
DR Antibodypedia; 32513; 56 antibodies from 11 providers.
DR Ensembl; ENSMUST00000169670; ENSMUSP00000131647; ENSMUSG00000039552.
DR GeneID; 212892; -.
DR KEGG; mmu:212892; -.
DR UCSC; uc007euh.2; mouse.
DR CTD; 345895; -.
DR MGI; MGI:3027894; Rsph4a.
DR VEuPathDB; HostDB:ENSMUSG00000039552; -.
DR eggNOG; ENOG502QSU4; Eukaryota.
DR GeneTree; ENSGT00500000044869; -.
DR HOGENOM; CLU_021526_1_0_1; -.
DR InParanoid; Q8BYM7; -.
DR OMA; AQFQKKM; -.
DR OrthoDB; 619686at2759; -.
DR PhylomeDB; Q8BYM7; -.
DR TreeFam; TF324531; -.
DR BioGRID-ORCS; 212892; 0 hits in 70 CRISPR screens.
DR ChiTaRS; Rsph4a; mouse.
DR PRO; PR:Q8BYM7; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q8BYM7; protein.
DR Bgee; ENSMUSG00000039552; Expressed in choroid plexus epithelium and 49 other tissues.
DR Genevisible; Q8BYM7; MM.
DR GO; GO:0005930; C:axoneme; ISO:MGI.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0001534; C:radial spoke; ISS:UniProtKB.
DR GO; GO:0001535; C:radial spoke head; IMP:MGI.
DR GO; GO:0035082; P:axoneme assembly; ISO:MGI.
DR GO; GO:0003341; P:cilium movement; ISO:MGI.
DR GO; GO:0060294; P:cilium movement involved in cell motility; IEA:InterPro.
DR GO; GO:0120221; P:maintenance of ciliary planar beating movement pattern; IMP:MGI.
DR GO; GO:0062177; P:radial spoke assembly; IMP:UniProtKB.
DR InterPro; IPR006802; Radial_spoke.
DR PANTHER; PTHR13159; PTHR13159; 1.
DR Pfam; PF04712; Radial_spoke; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell projection; Cilium; Cilium biogenesis/degradation;
KW Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome.
FT CHAIN 1..716
FT /note="Radial spoke head protein 4 homolog A"
FT /id="PRO_0000313739"
FT REGION 1..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 374..413
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 564..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 668..716
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..77
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 564..582
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 702..716
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 395
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT HELIX 278..290
FT /evidence="ECO:0007829|PDB:7DMP"
FT HELIX 314..324
FT /evidence="ECO:0007829|PDB:7DMP"
FT HELIX 332..344
FT /evidence="ECO:0007829|PDB:7DMP"
FT TURN 345..348
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 354..358
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 361..363
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 365..370
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 372..374
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 421..424
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 427..438
FT /evidence="ECO:0007829|PDB:7DMP"
FT TURN 448..450
FT /evidence="ECO:0007829|PDB:7DMP"
FT TURN 453..456
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 463..465
FT /evidence="ECO:0007829|PDB:7DMP"
FT HELIX 480..491
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 496..503
FT /evidence="ECO:0007829|PDB:7DMP"
FT HELIX 532..537
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 539..544
FT /evidence="ECO:0007829|PDB:7DMP"
FT HELIX 596..598
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 607..613
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 618..620
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 623..627
FT /evidence="ECO:0007829|PDB:7DMP"
FT TURN 628..632
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 634..637
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 642..648
FT /evidence="ECO:0007829|PDB:7DMP"
FT TURN 674..676
FT /evidence="ECO:0007829|PDB:7DMP"
FT HELIX 684..687
FT /evidence="ECO:0007829|PDB:7DMP"
FT TURN 688..692
FT /evidence="ECO:0007829|PDB:7DMP"
SQ SEQUENCE 716 AA; 80146 MW; F7C76BEFB1DDEAB8 CRC64;
MENSTSLKQE KENQEPGEAE RLWQGESDVS PQEPGPPSPE YREEEQRTDT EPAPRMSPSW
SHQSRVSLST GDLTAGPEVS SSPPPPPLQF HSTPLNTETT QDPVAASPTE KTANGIADTG
TPYSDPWESS SAAKQSTSHY TSHAEESTFP QSQTPQPDLC GLRDASRNKS KHKGLRFDLL
QEEGSDSNCD PDQPEVGASE AAQSMLEVAI QNAKAYLLST SSKSGLNLYD HLSKVLTKIL
DERPADAVDI IENISQDVKM AHFNKKLDTL HNEYEMLPAY EIAETQKALF LQGHLEGADS
ELEEEMAESS LPNVMESAYY FEQAGVGLGT DETYRVFLAL KQLTDTHPIQ RCRFWGKILG
LEMNYIVAEV EFRDGEDEEE VEEEGIAEER DNGGSEAGEE EEEELPKSLY KAPQVIPKEE
SRTGANKYVY FVCNVPGRPW VRLPSVTPAQ IVTARKIKKF FTGRLDAAVI SYPPFPGNES
NYLRAQIARI SAGTHVSPLG FYQFGEEEGE EEEVEGGRDS YEENPDFEGI QVIDLVESLS
NWVHHVQYIL PQGRCNWFNP IQKDEDEEEE EEEDEEKGEE PDYIEQEVGP PLLTPISEDL
GIQNIPSWTT QLSSNLIPQY AIAVLRSNLW PGAYAFSNGK KFENFYIGWG HKYCVENYTP
PSPPPVYQEY PSGPEITEMN DPSVEEEQAF RMTQEPVALS TEENEGTEDE DEDDED