RSIV_BACSU
ID RSIV_BACSU Reviewed; 285 AA.
AC O05403; Q795Y8;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Anti-sigma-V factor RsiV;
GN Name=rsiV; Synonyms=yrhM; OrderedLocusNames=BSU27130;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9308178; DOI=10.1099/00221287-143-9-2939;
RA Sorokin A., Bolotin A., Purnelle B., Hilbert H., Lauber J.,
RA Duesterhoeft A., Ehrlich S.D.;
RT "Sequence of the Bacillus subtilis genome region in the vicinity of the lev
RT operon reveals two new extracytoplasmic function RNA polymerase sigma
RT factors SigV and SigZ.";
RL Microbiology 143:2939-2943(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP INTERACTION WITH SIGV.
RC STRAIN=168;
RX PubMed=14993308; DOI=10.1099/mic.0.26712-0;
RA Yoshimura M., Asai K., Sadaie Y., Yoshikawa H.;
RT "Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma
RT factors with the N-terminal regions of their potential anti-sigma
RT factors.";
RL Microbiology 150:591-599(2004).
RN [4]
RP FUNCTION AS ANTI-FACTOR OF SIGV.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=16274938; DOI=10.1016/j.femsle.2005.09.056;
RA Zellmeier S., Hofmann C., Thomas S., Wiegert T., Schumann W.;
RT "Identification of sigmaV-dependent genes of Bacillus subtilis.";
RL FEMS Microbiol. Lett. 253:221-229(2005).
CC -!- FUNCTION: Anti-sigma factor for SigV. Negatively regulates SigV
CC activity through direct interaction. {ECO:0000269|PubMed:16274938}.
CC -!- SUBUNIT: Interacts (via N-terminal region) with SigV.
CC {ECO:0000269|PubMed:14993308}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RsiV family. {ECO:0000305}.
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DR EMBL; U93874; AAB80870.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14655.1; -; Genomic_DNA.
DR PIR; D69975; D69975.
DR RefSeq; NP_390591.1; NC_000964.3.
DR RefSeq; WP_004398482.1; NZ_JNCM01000036.1.
DR PDB; 5JEN; X-ray; 2.30 A; A/C=60-285.
DR PDBsum; 5JEN; -.
DR AlphaFoldDB; O05403; -.
DR SMR; O05403; -.
DR IntAct; O05403; 1.
DR STRING; 224308.BSU27130; -.
DR PaxDb; O05403; -.
DR PRIDE; O05403; -.
DR EnsemblBacteria; CAB14655; CAB14655; BSU_27130.
DR GeneID; 937593; -.
DR KEGG; bsu:BSU27130; -.
DR PATRIC; fig|224308.179.peg.2946; -.
DR eggNOG; ENOG502Z8Z7; Bacteria.
DR InParanoid; O05403; -.
DR OMA; GHLSIYS; -.
DR PhylomeDB; O05403; -.
DR BioCyc; BSUB:BSU27130-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 3.90.640.20; -; 1.
DR InterPro; IPR021729; DUF3298.
DR InterPro; IPR025303; PdaC.
DR InterPro; IPR037126; PdaC/RsiV-like_sf.
DR Pfam; PF11738; DUF3298; 1.
DR Pfam; PF13739; PdaC; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..285
FT /note="Anti-sigma-V factor RsiV"
FT /id="PRO_0000380591"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..285
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT STRAND 78..89
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 92..102
FT /evidence="ECO:0007829|PDB:5JEN"
FT HELIX 107..131
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 133..135
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 140..151
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 153..179
FT /evidence="ECO:0007829|PDB:5JEN"
FT TURN 180..183
FT /evidence="ECO:0007829|PDB:5JEN"
FT HELIX 189..191
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 192..194
FT /evidence="ECO:0007829|PDB:5JEN"
FT HELIX 197..215
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 222..224
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 240..242
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 248..252
FT /evidence="ECO:0007829|PDB:5JEN"
FT TURN 254..256
FT /evidence="ECO:0007829|PDB:5JEN"
FT HELIX 260..262
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 264..269
FT /evidence="ECO:0007829|PDB:5JEN"
FT HELIX 271..274
FT /evidence="ECO:0007829|PDB:5JEN"
FT HELIX 275..277
FT /evidence="ECO:0007829|PDB:5JEN"
FT STRAND 281..283
FT /evidence="ECO:0007829|PDB:5JEN"
SQ SEQUENCE 285 AA; 32868 MW; 3885E56ACA5B844D CRC64;
MDKRLQQLRE EYKNVQIPKE LDIIVEKALQ QEPKKKRIVM WPTSAAIAAA ILFTALVNIN
PDAAQAMSKI PVIGKIVKAI TFIEIKEEKD QSSIDVKTPA LSGLSNKELE NSINEKYLKE
SQQLYKEFIQ STSKNKKGHL SIYSDYETVT DTPDLLSIRR NIETTQASSY TQSRYITIDK
KNDILLTLKS LFKDERYIKV ISQNIKEQMK QQMKEDPNKI YWLTDEDAEP FKTILPDQTF
YITEDHKLVI SFDEYEVAPG YMGVTEFTIP TGVISNLLVG ERYIR
