RSIX_BACSU
ID RSIX_BACSU Reviewed; 368 AA.
AC P35166;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Anti-sigma-X factor RsiX;
DE AltName: Full=Sigma-X negative effector;
GN Name=rsiX; Synonyms=ypuN; OrderedLocusNames=BSU23090;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=7934829; DOI=10.1111/j.1365-2958.1993.tb02670.x;
RA Sorokin A.V., Zumstein E., Azevedo V., Ehrlich S.D., Serror P.;
RT "The organization of the Bacillus subtilis 168 chromosome region between
RT the spoVA and serA genetic loci, based on sequence data.";
RL Mol. Microbiol. 10:385-395(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 304.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP CHARACTERIZATION.
RX PubMed=9393439; DOI=10.1007/s004380050585;
RA Brutsche S., Braun V.;
RT "SigX of Bacillus subtilis replaces the ECF sigma factor fecI of
RT Escherichia coli and is inhibited by RsiX.";
RL Mol. Gen. Genet. 256:416-425(1997).
RN [5]
RP DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=22211522; DOI=10.1111/j.1365-2958.2011.07953.x;
RA Luo Y., Helmann J.D.;
RT "Analysis of the role of Bacillus subtilis sigma(M) in beta-lactam
RT resistance reveals an essential role for c-di-AMP in peptidoglycan
RT homeostasis.";
RL Mol. Microbiol. 83:623-639(2012).
CC -!- FUNCTION: The anti-sigma factor for extracytoplasmic function (ECF)
CC sigma factor SigX, inhibits SigX activity and stabilizes it.
CC {ECO:0000269|PubMed:9393439}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC -!- DISRUPTION PHENOTYPE: Insertion mutations in this gene partially
CC restore antibiotic resistance to the beta-lactam antibiotic cefuroxime
CC (CEF) in a sigM deletion mutant (PubMed:22211522).
CC {ECO:0000269|PubMed:22211522}.
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DR EMBL; L09228; AAA67500.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14241.2; -; Genomic_DNA.
DR PIR; S45562; S45562.
DR RefSeq; NP_390190.2; NC_000964.3.
DR RefSeq; WP_009967642.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P35166; -.
DR SMR; P35166; -.
DR IntAct; P35166; 1.
DR STRING; 224308.BSU23090; -.
DR PaxDb; P35166; -.
DR PRIDE; P35166; -.
DR EnsemblBacteria; CAB14241; CAB14241; BSU_23090.
DR GeneID; 938959; -.
DR KEGG; bsu:BSU23090; -.
DR PATRIC; fig|224308.179.peg.2516; -.
DR eggNOG; ENOG50331H4; Bacteria.
DR OMA; RWIGPAC; -.
DR BioCyc; BSUB:BSU23090-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..368
FT /note="Anti-sigma-X factor RsiX"
FT /id="PRO_0000097486"
FT REGION 73..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 304
FT /note="I -> M (in Ref. 1; AAA67500)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 368 AA; 41295 MW; DE9CD8C795077B3E CRC64;
MMKSEWNEEQ IKELLSQLPA VKDHRSPQDI YKRLTMAKRK NKPAVRWIGP ACAAAIAVYI
AFIISPHFFD QAQPQQKEAS QENAVTKTET EDSPKAASSL DQTSFVVPEK EQDNYITVAV
ADADTSAIIP VSIQKTNADQ TIQDMLFESS ELGILDHAIT IPTFIDEVEI KEKPKQKELS
IRVHQPATAF SIKDDTLLKK LLKESLKWSP YEKVKFLSDQ NETGVRIGSY GTFTEISIPK
QSKRSYYLYQ NKQGQDFLVP SNHSFDTVKE AIKEMESSSQ EDTTPLIQAG AVQSVTKKQK
HLYIRFSKES EVDDSIAGIL MIEGLLLTAK EFGFTEVTFT ETRTKKIGKY DISDAIPVPA
APNPISLN
