ID   RSKA_MYCBO              Reviewed;         232 AA.
AC   Q7U1Z7; A0A1R3XVD0; X2BF17;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Dysfunctional anti-sigma-K factor RskA;
DE   AltName: Full=Regulator of SigK;
DE   AltName: Full=Sigma-K anti-sigma factor RskA;
GN   Name=rskA; OrderedLocusNames=BQ2027_MB0452C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
RN   [3]
RP   LACK OF FUNCTION AS AN ANTI-SIGMA-K FACTOR.
RC   STRAIN=62389;
RX   PubMed=17064366; DOI=10.1111/j.1365-2958.2006.05455.x;
RA   Said-Salim B., Mostowy S., Kristof A.S., Behr M.A.;
RT   "Mutations in Mycobacterium tuberculosis Rv0444c, the gene encoding anti-
RT   sigK, explain high level expression of MPB70 and MPB83 in Mycobacterium
RT   bovis.";
RL   Mol. Microbiol. 62:1251-1263(2006).
CC   -!- FUNCTION: An anti-sigma factor for extracytoplasmic function (ECF)
CC       sigma factor SigK. ECF sigma factors are held in an inactive form by an
CC       anti-sigma factor until released by regulated intramembrane proteolysis
CC       (RIP). However, in M.bovis this protein is probably dysfunctional, due
CC       to at least 1 of the 2 naturally occurring polymorphisms in its gene,
CC       when compared to M.tuberculosis. This leads to an increased expression
CC       of SigK-regulated genes, such as mpb70 and mpb83. RIP occurs when an
CC       extracytoplasmic signal triggers a concerted proteolytic cascade to
CC       transmit information and elicit cellular responses. The membrane-
CC       spanning regulatory substrate protein is first cut extracytoplasmically
CC       (site-1 protease, S1P), then within the membrane itself (site-2
CC       protease, S2P, Rip1), while cytoplasmic proteases finish degrading the
CC       regulatory protein, liberating the sigma factor.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DOMAIN: The cytosolic domain interacts with sigma factor SigK.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the anti-sigma-K factor family. {ECO:0000305}.
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DR   EMBL; LT708304; SIT99040.1; -; Genomic_DNA.
DR   RefSeq; NP_854115.1; NC_002945.3.
DR   RefSeq; WP_010950404.1; NC_002945.4.
DR   AlphaFoldDB; Q7U1Z7; -.
DR   SMR; Q7U1Z7; -.
DR   PATRIC; fig|233413.5.peg.492; -.
DR   OMA; VYQMWLI; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 1.10.10.1320; -; 1.
DR   InterPro; IPR018764; Anti-sigma_K_RskA.
DR   InterPro; IPR041916; Anti_sigma_zinc_sf.
DR   Pfam; PF10099; RskA; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transcription; Transcription regulation;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..232
FT                   /note="Dysfunctional anti-sigma-K factor RskA"
FT                   /id="PRO_0000313830"
FT   TOPO_DOM        1..90
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        112..232
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   232 AA;  24013 MW;  84F732430EE3C7EA CRC64;
     MTEHTDFELL ELATPYALNA VSDDERADID RRVAAAPSPV AAAFNDEVRA VRETMAVVSA
     ATTAEPPAHL RTAILDATKP EVRRQSRWRT AAFASAAAIA VGLGAFDLGV LTRPSPPPTV
     AEQVLTAPDV RTVSRPLGAG TATVVFSRDR NTGLLVMNNV APPSRGTVYQ MWLLGGAKGP
     RSAETMGTAA VTPSTTATLT DLGASTALAF TVEPGTGSPQ PTGTILAELP LG