ID   RSKA_MYCTO              Reviewed;         232 AA.
AC   P9WGX4; L0T3K9; O53729; Q7D9T4;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Anti-sigma-K factor RskA;
DE   AltName: Full=Regulator of SigK;
DE   AltName: Full=Sigma-K anti-sigma factor RskA;
GN   Name=rskA; OrderedLocusNames=MT0460;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: An anti-sigma factor for extracytoplasmic function (ECF)
CC       sigma factor SigK. ECF sigma factors are held in an inactive form by an
CC       anti-sigma factor until released by regulated intramembrane proteolysis
CC       (RIP). RIP occurs when an extracytoplasmic signal triggers a concerted
CC       proteolytic cascade to transmit information and elicit cellular
CC       responses. The membrane-spanning regulatory substrate protein is first
CC       cut extracytoplasmically (site-1 protease, S1P), then within the
CC       membrane itself (site-2 protease, S2P, Rip1), while cytoplasmic
CC       proteases finish degrading the regulatory protein, liberating the sigma
CC       factor (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with ECF RNA polymerase sigma factor SigK; this
CC       inhibits the interaction of SigK with the RNA polymerase catalytic core
CC       and leads to a decreased expression of SigK-regulated genes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the anti-sigma-K factor family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK44683.1; -; Genomic_DNA.
DR   PIR; E70830; E70830.
DR   RefSeq; WP_003898455.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WGX4; -.
DR   SMR; P9WGX4; -.
DR   EnsemblBacteria; AAK44683; AAK44683; MT0460.
DR   KEGG; mtc:MT0460; -.
DR   PATRIC; fig|83331.31.peg.487; -.
DR   HOGENOM; CLU_075802_1_1_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 1.10.10.1320; -; 1.
DR   InterPro; IPR018764; Anti-sigma_K_RskA.
DR   InterPro; IPR041916; Anti_sigma_zinc_sf.
DR   Pfam; PF10099; RskA; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Transcription; Transcription regulation;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..232
FT                   /note="Anti-sigma-K factor RskA"
FT                   /id="PRO_0000428286"
FT   TOPO_DOM        1..90
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        112..232
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   232 AA;  23883 MW;  877A84993EE217E9 CRC64;
     MTEHTDFELL ELATPYALNA VSDDERADID RRVAAAPSPV AAAFNDEVRA VRETMAVVSA
     ATTAEPPAHL RTAILDATKP EVRRQSRWRT AAFASAAAIA VGLGAFGLGV LTRPSPPPTV
     AEQVLTAPDV RTVSRPLGAG TATVVFSRDR NTGLLVMNNV APPSRGTVYQ MWLLGGAKGP
     RSAGTMGTAA VTPSTTATLT DLGASTALAF TVEPGTGSPQ PTGTILAELP LG