RSLAB_MOUSE
ID RSLAB_MOUSE Reviewed; 203 AA.
AC Q5SSG5;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Ras-like protein family member 10B;
DE EC=3.6.5.2 {ECO:0000250|UniProtKB:P01116};
DE Flags: Precursor;
GN Name=Rasl10b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Visual cortex;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, INTERACTION WITH CADPS, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=17984325; DOI=10.1083/jcb.200707101;
RA Rybkin I.I., Kim M.S., Bezprozvannaya S., Qi X., Richardson J.A.,
RA Plato C.F., Hill J.A., Bassel-Duby R., Olson E.N.;
RT "Regulation of atrial natriuretic peptide secretion by a novel Ras-like
RT protein.";
RL J. Cell Biol. 179:527-537(2007).
CC -!- FUNCTION: May facilitate the release of atrial natriuretic peptide by
CC cardiomyocytes and hence play a role in the regulation of arterial
CC pressure. {ECO:0000269|PubMed:17984325}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC Evidence={ECO:0000250|UniProtKB:P01116};
CC -!- SUBUNIT: Interacts with CADPS. {ECO:0000269|PubMed:17984325}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in heart and brain. In the brain,
CC expression restricted to neurons of the cortex, hippocampus and
CC cerebellum. Sparse expression in the brainstem and almost absent in the
CC hypothalamic region. In the heart, expressed in cardiomyocytes. Not
CC detected in interstitial tissue or vascular cells.
CC {ECO:0000269|PubMed:17984325}.
CC -!- DEVELOPMENTAL STAGE: At 9.5 dpc, detected in the developing heart,
CC skeletal myotomes and dorsal portion of the neural tube. At 13.5 and
CC 15.5 dpc, expressed in the heart and neural tissue including the
CC central and peripheral nervous system. In the embryonic brain,
CC expression is restricted to more mature neurons and absent from the
CC progenitor neuronal cells of the ventricular zone. Also present in
CC mature peripheral neurons, such as the neurons of the dorsal root
CC ganglia and the Auerbach's and Meissner's plexi of the intestinal
CC tract. {ECO:0000269|PubMed:17984325}.
CC -!- DISRUPTION PHENOTYPE: No obvious phenotype. Mutant mice are viable and
CC fertile. {ECO:0000269|PubMed:17984325}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ras family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK158941; BAE34735.1; -; mRNA.
DR EMBL; AL663096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC116407; AAI16408.1; -; mRNA.
DR CCDS; CCDS25163.1; -.
DR RefSeq; NP_001013404.1; NM_001013386.2.
DR RefSeq; XP_006533548.1; XM_006533485.1.
DR RefSeq; XP_006533549.1; XM_006533486.1.
DR AlphaFoldDB; Q5SSG5; -.
DR SMR; Q5SSG5; -.
DR STRING; 10090.ENSMUSP00000132696; -.
DR iPTMnet; Q5SSG5; -.
DR PhosphoSitePlus; Q5SSG5; -.
DR PaxDb; Q5SSG5; -.
DR PeptideAtlas; Q5SSG5; -.
DR PRIDE; Q5SSG5; -.
DR ProteomicsDB; 260996; -.
DR Antibodypedia; 73077; 72 antibodies from 18 providers.
DR DNASU; 276952; -.
DR Ensembl; ENSMUST00000021022; ENSMUSP00000021022; ENSMUSG00000020684.
DR Ensembl; ENSMUST00000108140; ENSMUSP00000103775; ENSMUSG00000020684.
DR Ensembl; ENSMUST00000164425; ENSMUSP00000132696; ENSMUSG00000020684.
DR GeneID; 276952; -.
DR KEGG; mmu:276952; -.
DR UCSC; uc007kov.1; mouse.
DR CTD; 91608; -.
DR MGI; MGI:2685575; Rasl10b.
DR VEuPathDB; HostDB:ENSMUSG00000020684; -.
DR eggNOG; KOG0395; Eukaryota.
DR GeneTree; ENSGT00940000160764; -.
DR HOGENOM; CLU_041217_9_3_1; -.
DR InParanoid; Q5SSG5; -.
DR OMA; WPAVCES; -.
DR OrthoDB; 1283500at2759; -.
DR PhylomeDB; Q5SSG5; -.
DR TreeFam; TF325043; -.
DR BioGRID-ORCS; 276952; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q5SSG5; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5SSG5; protein.
DR Bgee; ENSMUSG00000020684; Expressed in superior frontal gyrus and 112 other tissues.
DR ExpressionAtlas; Q5SSG5; baseline and differential.
DR Genevisible; Q5SSG5; MM.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0090277; P:positive regulation of peptide hormone secretion; IDA:UniProtKB.
DR GO; GO:0003050; P:regulation of systemic arterial blood pressure by atrial natriuretic peptide; IMP:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW Cell membrane; GTP-binding; Hydrolase; Lipoprotein; Membrane; Methylation;
KW Nucleotide-binding; Prenylation; Reference proteome.
FT CHAIN 1..200
FT /note="Ras-like protein family member 10B"
FT /id="PRO_0000280801"
FT PROPEP 201..203
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000281363"
FT REGION 1..203
FT /note="Small GTPase-like"
FT MOTIF 33..42
FT /note="Effector region"
FT BINDING 11..18
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 59..62
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 128..131
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 200
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 200
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 203 AA; 23229 MW; 1EB70B99274C060A CRC64;
MVSTYRVAVL GARGVGKSAI VRQFLYNEFS EVCVPTTTRR LYLPAVVMNG HVHDLQILDF
PPISAFPVNT LQEWADACCR GLRSVHAYIL VYDICCFDSF EYVKTIRQQI LETRVIGTSE
TPIIIVGNKR DLQRGRVIPR WNVSHLVRKT WKCGYVECSA KYNWHILLLF SELLKSVGCA
RCKHVHAALR FQGALRRNRC AIM
