BCAS3_DROME
ID BCAS3_DROME Reviewed; 1122 AA.
AC Q8SY41;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Breast carcinoma-amplified sequence 3 homolog;
DE AltName: Full=Protein rudhira;
DE Short=DRudh;
GN Name=rudhira; ORFNames=CG43154;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP DOMAIN.
RX PubMed=16099728; DOI=10.1016/j.modgep.2005.06.002;
RA Siva K., Inamdar M.S.;
RT "Rudhira is a cytoplasmic WD40 protein expressed in mouse embryonic stem
RT cells and during embryonic erythropoiesis.";
RL Gene Expr. Patterns 6:225-234(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18355807; DOI=10.1016/j.yexcr.2008.02.009;
RA Das D., Aradhya R., Ashoka D., Inamdar M.;
RT "Macromolecular uptake in Drosophila pericardial cells requires rudhira
RT function.";
RL Exp. Cell Res. 314:1804-1810(2008).
RN [7]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=18060846; DOI=10.1016/j.gep.2007.10.008;
RA Das D., Ashoka D., Aradhya R., Inamdar M.;
RT "Gene expression analysis in post-embryonic pericardial cells of
RT Drosophila.";
RL Gene Expr. Patterns 8:199-205(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Regulates macropinocytosis in pericardial cells.
CC {ECO:0000269|PubMed:18355807}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18060846}.
CC -!- TISSUE SPECIFICITY: Expressed in all postembryonic pericardial cells,
CC but not in cardioblasts. Also expressed in Garland cells in third
CC instar larvae (at protein level). {ECO:0000269|PubMed:18060846}.
CC -!- DEVELOPMENTAL STAGE: Not expressed during embryonic development and
CC first instar larvae. Expression detected from second instar larval
CC stage into adulthood (at protein level). {ECO:0000269|PubMed:18060846}.
CC -!- DOMAIN: Has been proposed to contain 7 WD repeats. This prediction
CC could not be reproduced. {ECO:0000305|PubMed:16099728}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown of the protein in the
CC hematopoietic system and pericardial cells causes a decrease in
CC macropinocytic uptake by pericardial cells.
CC {ECO:0000269|PubMed:18355807}.
CC -!- SIMILARITY: Belongs to the BCAS3 family. {ECO:0000305}.
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DR EMBL; AE014298; AAN09302.1; -; Genomic_DNA.
DR EMBL; AY075392; AAL68226.1; -; mRNA.
DR RefSeq; NP_001245631.1; NM_001258702.1.
DR RefSeq; NP_001259463.1; NM_001272534.1.
DR RefSeq; NP_727567.1; NM_167309.3.
DR AlphaFoldDB; Q8SY41; -.
DR IntAct; Q8SY41; 2.
DR STRING; 7227.FBpp0294057; -.
DR iPTMnet; Q8SY41; -.
DR PaxDb; Q8SY41; -.
DR PRIDE; Q8SY41; -.
DR EnsemblMetazoa; FBtr0305603; FBpp0294054; FBgn0266019.
DR EnsemblMetazoa; FBtr0305605; FBpp0294056; FBgn0266019.
DR EnsemblMetazoa; FBtr0333015; FBpp0305229; FBgn0266019.
DR GeneID; 32136; -.
DR KEGG; dme:Dmel_CG43154; -.
DR UCSC; CG32663-RA; d. melanogaster.
DR CTD; 32136; -.
DR FlyBase; FBgn0266019; rudhira.
DR VEuPathDB; VectorBase:FBgn0266019; -.
DR eggNOG; KOG2109; Eukaryota.
DR eggNOG; KOG4415; Eukaryota.
DR GeneTree; ENSGT00390000006454; -.
DR HOGENOM; CLU_008458_1_0_1; -.
DR InParanoid; Q8SY41; -.
DR PhylomeDB; Q8SY41; -.
DR BioGRID-ORCS; 32136; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32136; -.
DR PRO; PR:Q8SY41; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0266019; Expressed in saliva-secreting gland and 22 other tissues.
DR ExpressionAtlas; Q8SY41; baseline and differential.
DR Genevisible; Q8SY41; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005829; C:cytosol; HDA:FlyBase.
DR GO; GO:0006914; P:autophagy; IEA:InterPro.
DR GO; GO:0044351; P:macropinocytosis; IMP:FlyBase.
DR GO; GO:0042594; P:response to starvation; IBA:GO_Central.
DR InterPro; IPR022175; BCAS3.
DR InterPro; IPR045142; BCAS3-like.
DR PANTHER; PTHR13268; PTHR13268; 1.
DR Pfam; PF12490; BCAS3; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..1122
FT /note="Breast carcinoma-amplified sequence 3 homolog"
FT /id="PRO_0000372650"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 357..382
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 620..644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 966..990
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1033..1054
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1071..1122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..37
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 357..377
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 625..644
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1087..1111
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 55
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 638
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 1122 AA; 117535 MW; 63E608BD1AA221E8 CRC64;
MSADSPRRHP SGVVGSGIGL GSGSGTGLGS GSTGGSKSGA AVPAIVPPQT VSDRSILDSA
IGFINDVTLA NQPVQDPKDT ITWARFETCA DVSDPRFGDD WELEGNAAPP LLLILGYGLG
VQVWAIPANG EAVEVLSWRH GVVTALRVLP TPATAAALDE NGRADEPVDS FAEKRPLVAF
VDGGSAAASG LLAGSSGLGL GGGGGGVTTV GGSVGGVSGI GVSASAQFSA VNFMSLKTGV
QVKTIKFKNA VLDIQANRSA VVITFHERIA VFDARTLEDR LTITTCYPSP GINPNPIALG
PRWLAYAEHK LLHSKRSGGG CDGEGVPSYT ATVLNAAKSL SKGLREFGEQ VAAGLTGTTA
GSGASSKSSS FDSASGGPDA KQSGVVTIID VKHPVKDYSP TSGTPLSSTA GSQGGGDPIV
AHFVAHSEAL VAMEFDSSGM LLLTADRRGH DFHVFRVQPH PVGPSLAAVH HLYVLHRGDT
SAKVQHIAFS LDSRWAAVST LRGTTHVFPI TPYGGAMGVR THTSLHVVNK LSRFHRSAGL
GADGRSSSPI SHSESTTFVQ SLQPYHNPTL PPYPRPSVVQ PLAQLRQPFT LGSPPGSAGL
GGGVGGGGVM GSGVSAGGHG VGVGVGSNSQ RQRQRLSSLS DDSGKPLSVC SIFAKSRSWL
LEPPMATREQ PHRVQRKAVD SLFVMAGHGA LIQYDLDTKL ASHVAKEKIC DDTPIELEVE
ARAQWNLGRR KDGSQEIAPP LGLDNWLIKD RHASLLLDSA NQFDDPDERT ESWLAQVEII
THAGPHRRLW MGPQFVFKNY NTPSGSNLNH VDAEAVEIGV SKTTTTTLPS TAASSALGLG
TIIGKDRSSP LNMPLSAATS VGGAGIGSSA VTGRSGAGVP VLIESGSYSS IEQSPKLMDR
FRHGHLDSDY GHGDTRLKED LADAMRESPS TAAARRETTG NYFTTDQLDG LALNNNNNIN
NNIIPTKDNA SPNPNTNTNP NAIPSSNKVQ KAEVTDAVDY PIRHSYGDHG ELSTVVNIEV
FDDQLSMSSI STNSRLSLEG PPSQSSPPLS LTNGLMDTNL MHFSESVTGT GVAQAQVHGR
GANRFEVDDD DEEEEEEEEE LDEEAEPDDD EREDRPLGRR NL
