ID   BCA_STRA1               Reviewed;        1020 AA.
AC   Q02192; Q3K2V1;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 136.
DE   RecName: Full=C protein alpha-antigen;
DE   Flags: Precursor;
GN   Name=bca; OrderedLocusNames=SAK_0517;
OS   Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC
OS   SS700).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=205921;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 27591 / A909 / CDC SS700;
RX   PubMed=1438195; DOI=10.1073/pnas.89.21.10060;
RA   Michel J.L., Madoff L.C., Olson K., Kling D.E., Kasper D.L., Ausubel F.M.;
RT   "Large, identical, tandem repeating units in the C protein alpha antigen
RT   gene, bca, of group B streptococci.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:10060-10064(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27591 / A909 / CDC SS700;
RX   PubMed=16172379; DOI=10.1073/pnas.0506758102;
RA   Tettelin H., Masignani V., Cieslewicz M.J., Donati C., Medini D.,
RA   Ward N.L., Angiuoli S.V., Crabtree J., Jones A.L., Durkin A.S., DeBoy R.T.,
RA   Davidsen T.M., Mora M., Scarselli M., Margarit y Ros I., Peterson J.D.,
RA   Hauser C.R., Sundaram J.P., Nelson W.C., Madupu R., Brinkac L.M.,
RA   Dodson R.J., Rosovitz M.J., Sullivan S.A., Daugherty S.C., Haft D.H.,
RA   Selengut J., Gwinn M.L., Zhou L., Zafar N., Khouri H., Radune D.,
RA   Dimitrov G., Watkins K., O'Connor K.J., Smith S., Utterback T.R., White O.,
RA   Rubens C.E., Grandi G., Madoff L.C., Kasper D.L., Telford J.L.,
RA   Wessels M.R., Rappuoli R., Fraser C.M.;
RT   "Genome analysis of multiple pathogenic isolates of Streptococcus
RT   agalactiae: implications for the microbial 'pan-genome'.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13950-13955(2005).
CC   -!- FUNCTION: May play a role in both virulence and immunity.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}.
CC   -!- MISCELLANEOUS: Identical repeating units define protective epitopes and
CC       may play a role in generating phenotypic and genotypic diversity.
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DR   EMBL; M97256; AAA26848.1; -; Genomic_DNA.
DR   EMBL; CP000114; ABA45154.1; -; Genomic_DNA.
DR   RefSeq; WP_000489955.1; NC_007432.1.
DR   PDB; 1YWM; X-ray; 1.86 A; A=52-225.
DR   PDB; 2O0I; X-ray; 3.10 A; 1=57-225.
DR   PDBsum; 1YWM; -.
DR   PDBsum; 2O0I; -.
DR   AlphaFoldDB; Q02192; -.
DR   SMR; Q02192; -.
DR   DrugBank; DB04447; 1,4-Dithiothreitol.
DR   KEGG; sak:SAK_0517; -.
DR   HOGENOM; CLU_311202_0_0_9; -.
DR   OMA; GKDQQVN; -.
DR   EvolutionaryTrace; Q02192; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1270.150; -; 1.
DR   Gene3D; 2.60.500.10; -; 1.
DR   InterPro; IPR038335; ACP_C_sf.
DR   InterPro; IPR038544; ACP_N_sf.
DR   InterPro; IPR035327; AlphaC_C.
DR   InterPro; IPR014933; AlphaC_N.
DR   InterPro; IPR019931; LPXTG_anchor.
DR   InterPro; IPR019950; M_anchor.
DR   InterPro; IPR012706; Rib_alpha_Esp.
DR   InterPro; IPR005877; YSIRK_signal_dom.
DR   Pfam; PF08829; AlphaC_N; 1.
DR   Pfam; PF17480; AlphaC_N2; 1.
DR   Pfam; PF00746; Gram_pos_anchor; 1.
DR   Pfam; PF04650; YSIRK_signal; 1.
DR   PRINTS; PR00015; GPOSANCHOR.
DR   TIGRFAMs; TIGR02331; rib_alpha; 9.
DR   TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR   PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell wall; Peptidoglycan-anchor; Repeat; Secreted; Signal;
KW   Virulence.
FT   SIGNAL          1..41
FT   CHAIN           42..990
FT                   /note="C protein alpha-antigen"
FT                   /id="PRO_0000005597"
FT   PROPEP          991..1020
FT                   /note="Removed by sortase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT                   /id="PRO_0000005598"
FT   REGION          227..964
FT                   /note="9 X 82 AA tandem repeats"
FT   REGION          261..281
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          306..330
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          342..363
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          388..445
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          470..494
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          506..527
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          552..576
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          588..610
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          634..658
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          670..692
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          716..740
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          752..774
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          798..822
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          834..856
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          880..904
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          962..989
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           987..991
FT                   /note="LPXTG sorting signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT   MOD_RES         990
FT                   /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT   STRAND          63..66
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   TURN            71..73
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   STRAND          78..82
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   HELIX           83..87
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   HELIX           93..96
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   STRAND          97..99
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   STRAND          104..111
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   STRAND          114..118
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   HELIX           119..121
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   STRAND          126..135
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   STRAND          139..141
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   STRAND          147..149
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   STRAND          151..159
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   HELIX           162..175
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   HELIX           182..201
FT                   /evidence="ECO:0007829|PDB:1YWM"
FT   HELIX           206..220
FT                   /evidence="ECO:0007829|PDB:1YWM"
SQ   SEQUENCE   1020 AA;  108705 MW;  78035594FA190D5B CRC64;
     MFRRSKNNSY DTSQTKQRFS IKKFKFGAAS VLIGLSFLGG VTQGNLNIFE ESIVAASTIP
     GSAATLNTSI TKNIQNGNAY IDLYDVKLGK IDPLQLIVLE QGFTAKYVFR QGTKYYGDVS
     QLQSTGRASL TYNIFGEDGL PHVKTDGQID IVSVALTIYD STTLRDKIEE VRTNANDPKW
     TEESRTEVLT GLDTIKTDID NNPKTQTDID SKIVEVNELE KLLVLSVPDK DKYDPTGGET
     TVPQGTPVSD KEITDLVKIP DGSKGVPTVV GDRPDTNVPG DHKVTVEVTY PDGTKDTVEV
     TVHVTPKPVP DKDKYDPTGG ETTVPQGTPV SDKEITDLVK IPDGSKGVPT VVGDRPDTNV
     PGDHKVTVEV TYPDGTKDTV EVTVHVTPKP VPDKDKYDPT GGETTVPQGT PVSDKEITDL
     VKIPDGSKGV PTVVGDRPDT NVPGDHKVTV EVTYPDGTKD TVEVTVHVTP KPVPDKDKYD
     PTGGETTVPQ GTPVSDKEIT DLVKIPDGSK GVPTVVGDRP DTNVPGDHKV TVEVTYPDGT
     KDTVEVTVHV TPKPVPDKDK YDPTGGETTV PQGTPVSDKE ITDLVKIPDG SKGVPTVVGD
     RPDTNVPGDH KVTVEVTYPD GTKDTVEVTV HVTPKPVPDK DKYDPTGGET TVPQGTPVSD
     KEITDLVKIP DGSKGVPTVV GDRPDTNVPG DHKVTVEVTY PDGTKDTVEV TVHVTPKPVP
     DKDKYDPTGG ETTVPQGTPV SDKEITDLVK IPDGSKGVPT VVGDRPDTNV PGDHKVTVEV
     TYPDGTKDTV EVTVHVTPKP VPDKDKYDPT GGETTVPQGT PVSDKEITDL VKIPDGSKGV
     PTVVGDRPDT NVPGDHKVTV EVTYPDGTKD TVEVTVHVTP KPVPDKDKYD PTGGETTVPQ
     GTPVSDKEIT DLVKIPDGSK GVPTVVGDRP DTNVPGDHKV TVEVTYPDGT KDTVEVTVHV
     TPKPVPDKDK YDPTGKAQQV NGKGNKLPAT GENATPFFNV AALTIISSVG LLSVSKKKED