ID   RSMD_BUCAI              Reviewed;         191 AA.
AC   P57136;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase D;
DE            EC=2.1.1.171;
DE   AltName: Full=16S rRNA m2G966 methyltransferase;
DE   AltName: Full=rRNA (guanine-N(2)-)-methyltransferase;
GN   Name=rsmD; OrderedLocusNames=BU023;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Specifically methylates the guanine in position 966 of 16S
CC       rRNA in the assembled 30S particle. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(966) in 16S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(966) in 16S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:23548, Rhea:RHEA-COMP:10211, Rhea:RHEA-COMP:10212,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.171;
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmD family.
CC       {ECO:0000305}.
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DR   EMBL; BA000003; BAB12750.1; -; Genomic_DNA.
DR   RefSeq; NP_239864.1; NC_002528.1.
DR   RefSeq; WP_009873984.1; NC_002528.1.
DR   AlphaFoldDB; P57136; -.
DR   SMR; P57136; -.
DR   STRING; 107806.10038715; -.
DR   EnsemblBacteria; BAB12750; BAB12750; BAB12750.
DR   KEGG; buc:BU023; -.
DR   PATRIC; fig|107806.10.peg.35; -.
DR   eggNOG; COG0742; Bacteria.
DR   HOGENOM; CLU_075826_2_2_6; -.
DR   OMA; FNWLMPY; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0052913; F:16S rRNA (guanine(966)-N(2))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR004398; RNA_MeTrfase_RsmD.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PIRSF; PIRSF004553; CHP00095; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR00095; TIGR00095; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..191
FT                   /note="Ribosomal RNA small subunit methyltransferase D"
FT                   /id="PRO_0000216243"
SQ   SEQUENCE   191 AA;  22651 MW;  436C5F084F13D420 CRC64;
     MNNSFFRKNS KIYIISGNLK GRKISFKNIP NLRPTTNQIR ETLFEWLSKY IKNSRCLDCF
     AGSGVLGIEA ISRYAAFSTL LEIEKKTFLT LKKNIKELNI YNVEIIRTNT LHWLKKTRNK
     PYDIIFIDPP YHQGLVKKTI NLLENKKWIK KNSFIYIEQE KKQSIIVPKN WTLYKKKITN
     QIQCYLYICS T