ID   RSMD_ECOL6              Reviewed;         198 AA.
AC   P0ADY0; P10120;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase D;
DE            EC=2.1.1.171;
DE   AltName: Full=16S rRNA m2G966 methyltransferase;
DE   AltName: Full=rRNA (guanine-N(2)-)-methyltransferase;
GN   Name=rsmD; OrderedLocusNames=c4258;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Specifically methylates the guanine in position 966 of 16S
CC       rRNA in the assembled 30S particle. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(966) in 16S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(966) in 16S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:23548, Rhea:RHEA-COMP:10211, Rhea:RHEA-COMP:10212,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.171;
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmD family.
CC       {ECO:0000305}.
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DR   EMBL; AE014075; AAN82694.1; -; Genomic_DNA.
DR   RefSeq; WP_000743193.1; NC_004431.1.
DR   AlphaFoldDB; P0ADY0; -.
DR   SMR; P0ADY0; -.
DR   STRING; 199310.c4258; -.
DR   EnsemblBacteria; AAN82694; AAN82694; c4258.
DR   GeneID; 66672651; -.
DR   KEGG; ecc:c4258; -.
DR   eggNOG; COG0742; Bacteria.
DR   HOGENOM; CLU_075826_2_2_6; -.
DR   OMA; FNWLMPY; -.
DR   BioCyc; ECOL199310:C4258-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0052913; F:16S rRNA (guanine(966)-N(2))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR004398; RNA_MeTrfase_RsmD.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PIRSF; PIRSF004553; CHP00095; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR00095; TIGR00095; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; rRNA processing; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..198
FT                   /note="Ribosomal RNA small subunit methyltransferase D"
FT                   /id="PRO_0000169549"
SQ   SEQUENCE   198 AA;  21678 MW;  04FB9D9DAF972605 CRC64;
     MKKPNHSGSG QIRIIGGQWR GRKLPVPDSP GLRPTTDRVR ETLFNWLAPV IVDAQCLDCF
     AGSGALGLEA LSRYAAGATL IEMDRAVSQQ LIKNLATLKA GNARVVNSNA MSFLAQKGTP
     HNIVFVDPPF RRGLLEETIN LLEDNGWLAD EALIYVESEV ENGLPTVPAN WSLHREKVAG
     QVAYRLYQRE AQGESDAD