RSMD_HAEIN
ID RSMD_HAEIN Reviewed; 193 AA.
AC P44869;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase D;
DE EC=2.1.1.171;
DE AltName: Full=16S rRNA m2G966 methyltransferase;
DE AltName: Full=rRNA (guanine-N(2)-)-methyltransferase;
GN Name=rsmD; OrderedLocusNames=HI_0767;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RG Northeast structural genomics consortium (NESG);
RT "Crystal structure of the putative methylase HI0767 from Haemophilus
RT influenzae.";
RL Submitted (OCT-2006) to the PDB data bank.
CC -!- FUNCTION: Specifically methylates the guanine in position 966 of 16S
CC rRNA in the assembled 30S particle. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(966) in 16S rRNA + S-adenosyl-L-methionine = H(+) +
CC N(2)-methylguanosine(966) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:23548, Rhea:RHEA-COMP:10211, Rhea:RHEA-COMP:10212,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.171;
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmD family.
CC {ECO:0000305}.
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DR EMBL; L42023; AAC22425.1; -; Genomic_DNA.
DR PIR; F64158; F64158.
DR RefSeq; NP_438926.1; NC_000907.1.
DR RefSeq; WP_005693161.1; NC_000907.1.
DR PDB; 2IFT; X-ray; 2.30 A; A/B=1-193.
DR PDBsum; 2IFT; -.
DR AlphaFoldDB; P44869; -.
DR SMR; P44869; -.
DR STRING; 71421.HI_0767; -.
DR DNASU; 949786; -.
DR EnsemblBacteria; AAC22425; AAC22425; HI_0767.
DR KEGG; hin:HI_0767; -.
DR PATRIC; fig|71421.8.peg.806; -.
DR eggNOG; COG0742; Bacteria.
DR HOGENOM; CLU_075826_2_2_6; -.
DR OMA; FNWLMPY; -.
DR PhylomeDB; P44869; -.
DR BioCyc; HINF71421:G1GJ1-807-MON; -.
DR EvolutionaryTrace; P44869; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0052913; F:16S rRNA (guanine(966)-N(2))-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004398; RNA_MeTrfase_RsmD.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PIRSF; PIRSF004553; CHP00095; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR00095; TIGR00095; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Methyltransferase; Reference proteome; rRNA processing;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..193
FT /note="Ribosomal RNA small subunit methyltransferase D"
FT /id="PRO_0000169550"
FT STRAND 12..14
FT /evidence="ECO:0007829|PDB:2IFT"
FT TURN 19..22
FT /evidence="ECO:0007829|PDB:2IFT"
FT STRAND 24..26
FT /evidence="ECO:0007829|PDB:2IFT"
FT HELIX 39..52
FT /evidence="ECO:0007829|PDB:2IFT"
FT STRAND 56..59
FT /evidence="ECO:0007829|PDB:2IFT"
FT HELIX 66..73
FT /evidence="ECO:0007829|PDB:2IFT"
FT STRAND 77..82
FT /evidence="ECO:0007829|PDB:2IFT"
FT HELIX 86..98
FT /evidence="ECO:0007829|PDB:2IFT"
FT TURN 103..105
FT /evidence="ECO:0007829|PDB:2IFT"
FT STRAND 106..109
FT /evidence="ECO:0007829|PDB:2IFT"
FT HELIX 113..116
FT /evidence="ECO:0007829|PDB:2IFT"
FT STRAND 126..131
FT /evidence="ECO:0007829|PDB:2IFT"
FT STRAND 135..137
FT /evidence="ECO:0007829|PDB:2IFT"
FT HELIX 139..149
FT /evidence="ECO:0007829|PDB:2IFT"
FT STRAND 153..167
FT /evidence="ECO:0007829|PDB:2IFT"
FT STRAND 175..183
FT /evidence="ECO:0007829|PDB:2IFT"
FT STRAND 186..193
FT /evidence="ECO:0007829|PDB:2IFT"
SQ SEQUENCE 193 AA; 22001 MW; C0D6FC3CF0A831B7 CRC64;
MKKIQTPNAK GEVRIIAGLW RGRKLPVLNS EGLRPTGDRV KETLFNWLMP YIHQSECLDG
FAGSGSLGFE ALSRQAKKVT FLELDKTVAN QLKKNLQTLK CSSEQAEVIN QSSLDFLKQP
QNQPHFDVVF LDPPFHFNLA EQAISLLCEN NWLKPNALIY VETEKDKPLI TPENWTLLKE
KTTGIVSYRL YQN
