ID   RSME_BUCAP              Reviewed;         246 AA.
AC   Q8K9E4;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase E;
DE            EC=2.1.1.193;
DE   AltName: Full=16S rRNA m3U1498 methyltransferase;
GN   Name=rsmE; OrderedLocusNames=BUsg_395;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC       uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC       ribosomal subunit (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC         N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC       {ECO:0000305}.
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DR   EMBL; AE013218; AAM67946.1; -; Genomic_DNA.
DR   RefSeq; WP_011053913.1; NC_004061.1.
DR   AlphaFoldDB; Q8K9E4; -.
DR   SMR; Q8K9E4; -.
DR   STRING; 198804.BUsg_395; -.
DR   EnsemblBacteria; AAM67946; AAM67946; BUsg_395.
DR   KEGG; bas:BUsg_395; -.
DR   eggNOG; COG1385; Bacteria.
DR   HOGENOM; CLU_067442_5_1_6; -.
DR   OMA; TQWKGER; -.
DR   OrthoDB; 1490892at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR006700; rRNA_ssu_MeTrfase-E.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR30027; PTHR30027; 1.
DR   Pfam; PF04452; Methyltrans_RNA; 1.
DR   PIRSF; PIRSF015601; MTase_slr0722; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00046; TIGR00046; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; rRNA processing; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..246
FT                   /note="Ribosomal RNA small subunit methyltransferase E"
FT                   /id="PRO_0000176209"
SQ   SEQUENCE   246 AA;  28743 MW;  E6F7F046C0F728E7 CRC64;
     MKKRIPRIYI KYFLQINQVI SLSKSYTHYI KKVLRMKKKD KLEIFNNTNY IFFSEILEIN
     NHTIEIIILK KQIKNLESPL LIHLGQVISK NEKMNFSIQK SVELGVNTIT PLFSEYCNFQ
     NKLICFPKKK IHWENIVISS CEQCHRNNIP EIKNPEYILP WCKRTYENET KIVFSPSATF
     TINELPEKIN CVRLLIGSEG GFSFLEIEKI IQYGFIPIKL GPRILRTETA VIAAITALQI
     KFGDLS