ID   RSME_MESH2              Reviewed;         227 AA.
AC   O50188; Q601M1;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase E;
DE            EC=2.1.1.193;
DE   AltName: Full=16S rRNA m3U1498 methyltransferase;
GN   Name=rsmE; OrderedLocusNames=mhp181;
OS   Mesomycoplasma hyopneumoniae (strain 232) (Mycoplasma hyopneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mesomycoplasma.
OX   NCBI_TaxID=295358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9729120; DOI=10.1016/s0378-1119(98)00247-9;
RA   Hsu T., Minion F.C.;
RT   "Molecular analysis of the P97 cilium adhesin operon of Mycoplasma
RT   hyopneumoniae.";
RL   Gene 214:13-23(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=232;
RX   PubMed=15489423; DOI=10.1128/jb.186.21.7123-7133.2004;
RA   Minion F.C., Lefkowitz E.J., Madsen M.L., Cleary B.J., Swartzell S.M.,
RA   Mahairas G.G.;
RT   "The genome sequence of Mycoplasma hyopneumoniae strain 232, the agent of
RT   swine mycoplasmosis.";
RL   J. Bacteriol. 186:7123-7133(2004).
CC   -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC       uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC       ribosomal subunit (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC         N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC       {ECO:0000305}.
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DR   EMBL; AF012905; AAC32528.1; -; Genomic_DNA.
DR   EMBL; AE017332; AAV27441.1; -; Genomic_DNA.
DR   PIR; T03807; T03807.
DR   RefSeq; WP_011206018.1; NC_006360.1.
DR   AlphaFoldDB; O50188; -.
DR   SMR; O50188; -.
DR   STRING; 295358.mhp181; -.
DR   EnsemblBacteria; AAV27441; AAV27441; mhp181.
DR   KEGG; mhy:mhp181; -.
DR   eggNOG; COG1385; Bacteria.
DR   HOGENOM; CLU_067442_5_1_14; -.
DR   OMA; IGPEGCF; -.
DR   PhylomeDB; O50188; -.
DR   Proteomes; UP000006822; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR006700; rRNA_ssu_MeTrfase-E.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR30027; PTHR30027; 1.
DR   Pfam; PF04452; Methyltrans_RNA; 1.
DR   PIRSF; PIRSF015601; MTase_slr0722; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00046; TIGR00046; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; rRNA processing; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..227
FT                   /note="Ribosomal RNA small subunit methyltransferase E"
FT                   /id="PRO_0000176212"
SQ   SEQUENCE   227 AA;  26286 MW;  799B5EE86BC3F71E CRC64;
     MYRFFVNEKQ ENFFILTNLT LNHIKTVRIK NENFICVYQN EFYLVRLVPN SNKAEIIEKI
     KGNNEPKNKV VLALAILKTK SFEFAIQKAV EIGVNEIWPF YSKNVSQKLS GDLEKKLKRW
     EQICLHSAQQ SFRNLIPKIN LPINYKDLLE QAKNFPVKLI SFERAKNNIN IPDNPQNTII
     IIGPEGGFDD VEIQQAEKLG FQSITLGKRI LRSETAAIFL LTKCIKD