ID   RSME_MYCBO              Reviewed;         262 AA.
AC   P67203; A0A1R3Y1T8; O05826; X2BKW0;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase E;
DE            EC=2.1.1.193;
DE   AltName: Full=16S rRNA m3U1498 methyltransferase;
GN   Name=rsmE; OrderedLocusNames=BQ2027_MB2393C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC       uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC       ribosomal subunit (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC         N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIU01005.1; -; Genomic_DNA.
DR   RefSeq; NP_856042.1; NC_002945.3.
DR   RefSeq; WP_003900509.1; NC_002945.4.
DR   AlphaFoldDB; P67203; -.
DR   SMR; P67203; -.
DR   EnsemblBacteria; SIU01005; SIU01005; BQ2027_MB2393C.
DR   PATRIC; fig|233413.5.peg.2630; -.
DR   OMA; TQWKGER; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR006700; rRNA_ssu_MeTrfase-E.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR30027; PTHR30027; 1.
DR   Pfam; PF04452; Methyltrans_RNA; 1.
DR   PIRSF; PIRSF015601; MTase_slr0722; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00046; TIGR00046; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; rRNA processing; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..262
FT                   /note="Ribosomal RNA small subunit methyltransferase E"
FT                   /id="PRO_0000176214"
SQ   SEQUENCE   262 AA;  27653 MW;  27F970FBF989B2BF CRC64;
     MVAMLFYVDT LPDTGAVAVV DGDEGFHAAT VRRIRPGEQL VLGDGVGRLA RCVVEQAGRG
     GLRARVLRRW SVPPVRPPVT VVQALPKSER SELAIELATE AGADAFLAWQ AARCVANWDG
     ARVDKGLRRW RAVVRSAARQ SRRARIPPVD GVLSTPMLVQ RVREEVAAGA AVLVLHEEAT
     ERIVDIAAAQ AGSLMLVVGP EGGIAPDELA ALTDAGAVAV RLGPTVLRTS TAAAVALGAV
     GVLTSRWDAS ASDCEYCDVT RR