RSME_MYCTU
ID RSME_MYCTU Reviewed; 262 AA.
AC P9WGX1; L0T9M1; O05826; P67202;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 37.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase E;
DE EC=2.1.1.193;
DE AltName: Full=16S rRNA m3U1498 methyltransferase;
GN Name=rsmE; OrderedLocusNames=Rv2372c; ORFNames=MTCY27.08;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA Raman K., Yeturu K., Chandra N.;
RT "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT through an interactome, reactome and genome-scale structural analysis.";
RL BMC Syst. Biol. 2:109-109(2008).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC ribosomal subunit (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP45160.1; -; Genomic_DNA.
DR PIR; C70587; C70587.
DR RefSeq; NP_216888.1; NC_000962.3.
DR RefSeq; WP_003900509.1; NZ_NVQJ01000029.1.
DR PDB; 4L69; X-ray; 2.90 A; A=1-262.
DR PDBsum; 4L69; -.
DR AlphaFoldDB; P9WGX1; -.
DR SMR; P9WGX1; -.
DR STRING; 83332.Rv2372c; -.
DR PaxDb; P9WGX1; -.
DR DNASU; 885926; -.
DR GeneID; 885926; -.
DR KEGG; mtu:Rv2372c; -.
DR TubercuList; Rv2372c; -.
DR eggNOG; COG1385; Bacteria.
DR OMA; TQWKGER; -.
DR PhylomeDB; P9WGX1; -.
DR BRENDA; 2.1.1.193; 3445.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070042; F:rRNA (uridine-N3-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR Gene3D; 3.40.1280.10; -; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR006700; rRNA_ssu_MeTrfase-E.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR PANTHER; PTHR30027; PTHR30027; 1.
DR Pfam; PF04452; Methyltrans_RNA; 1.
DR PIRSF; PIRSF015601; MTase_slr0722; 1.
DR SUPFAM; SSF75217; SSF75217; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00046; TIGR00046; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Methyltransferase; Reference proteome;
KW rRNA processing; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..262
FT /note="Ribosomal RNA small subunit methyltransferase E"
FT /id="PRO_0000176213"
FT STRAND 5..7
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 17..20
FT /evidence="ECO:0007829|PDB:4L69"
FT HELIX 24..29
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 39..43
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 45..56
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 62..71
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 79..84
FT /evidence="ECO:0007829|PDB:4L69"
FT HELIX 89..100
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:4L69"
FT TURN 112..114
FT /evidence="ECO:0007829|PDB:4L69"
FT HELIX 120..140
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 149..153
FT /evidence="ECO:0007829|PDB:4L69"
FT HELIX 155..167
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 170..175
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 180..186
FT /evidence="ECO:0007829|PDB:4L69"
FT TURN 188..190
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 192..198
FT /evidence="ECO:0007829|PDB:4L69"
FT HELIX 206..215
FT /evidence="ECO:0007829|PDB:4L69"
FT STRAND 218..221
FT /evidence="ECO:0007829|PDB:4L69"
FT TURN 229..231
FT /evidence="ECO:0007829|PDB:4L69"
FT HELIX 232..243
FT /evidence="ECO:0007829|PDB:4L69"
FT TURN 245..247
FT /evidence="ECO:0007829|PDB:4L69"
SQ SEQUENCE 262 AA; 27653 MW; 27F970FBF989B2BF CRC64;
MVAMLFYVDT LPDTGAVAVV DGDEGFHAAT VRRIRPGEQL VLGDGVGRLA RCVVEQAGRG
GLRARVLRRW SVPPVRPPVT VVQALPKSER SELAIELATE AGADAFLAWQ AARCVANWDG
ARVDKGLRRW RAVVRSAARQ SRRARIPPVD GVLSTPMLVQ RVREEVAAGA AVLVLHEEAT
ERIVDIAAAQ AGSLMLVVGP EGGIAPDELA ALTDAGAVAV RLGPTVLRTS TAAAVALGAV
GVLTSRWDAS ASDCEYCDVT RR
