ID   RSME_SHIFL              Reviewed;         243 AA.
AC   P0AGL9; P37912; P76647;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase E;
DE            EC=2.1.1.193;
DE   AltName: Full=16S rRNA m3U1498 methyltransferase;
GN   Name=rsmE; OrderedLocusNames=SF2937, S3141;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC       uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC       ribosomal subunit (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC         N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC       {ECO:0000305}.
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DR   EMBL; AE005674; AAN44418.2; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18243.1; -; Genomic_DNA.
DR   RefSeq; NP_708711.2; NC_004337.2.
DR   RefSeq; WP_001222509.1; NZ_WPGW01000085.1.
DR   AlphaFoldDB; P0AGL9; -.
DR   SMR; P0AGL9; -.
DR   STRING; 198214.SF2937; -.
DR   EnsemblBacteria; AAN44418; AAN44418; SF2937.
DR   EnsemblBacteria; AAP18243; AAP18243; S3141.
DR   GeneID; 1025941; -.
DR   GeneID; 60901714; -.
DR   KEGG; sfl:SF2937; -.
DR   KEGG; sfx:S3141; -.
DR   PATRIC; fig|198214.7.peg.3494; -.
DR   HOGENOM; CLU_067442_5_1_6; -.
DR   OMA; TQWKGER; -.
DR   OrthoDB; 1490892at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR006700; rRNA_ssu_MeTrfase-E.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR30027; PTHR30027; 1.
DR   Pfam; PF04452; Methyltrans_RNA; 1.
DR   PIRSF; PIRSF015601; MTase_slr0722; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00046; TIGR00046; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..243
FT                   /note="Ribosomal RNA small subunit methyltransferase E"
FT                   /id="PRO_0000176201"
SQ   SEQUENCE   243 AA;  26978 MW;  5C4659F5B295E033 CRC64;
     MRIPRIYHPE PLTSHSHIAL CEDAANHIGR VLRMGPGQAL QLFDGSNQVF DAEITSASKK
     SVEVKVLEGQ IDDRESPLHI HLGQVMSRGE KMEFTIQKSI ELGVSLITPL FSERCGVKLD
     SERLNKKLQQ WQKIAIAACE QCGRNRVPEI RPAMDLEAWC AEQDEGLKLN LHPRASNSIN
     TLPLPVERVR LLIGPEGGLS ADEIAMTARY QFTDILLGPR VLRTETTALT AITALQVRFG
     DLG