RSME_SYNY3
ID RSME_SYNY3 Reviewed; 303 AA.
AC P72667;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase E;
DE EC=2.1.1.193;
DE AltName: Full=16S rRNA m3U1498 methyltransferase;
GN Name=rsmE; OrderedLocusNames=slr0722;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
CC -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC ribosomal subunit (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC {ECO:0000305}.
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DR EMBL; BA000022; BAA16669.1; -; Genomic_DNA.
DR PIR; S74517; S74517.
DR AlphaFoldDB; P72667; -.
DR SMR; P72667; -.
DR IntAct; P72667; 3.
DR STRING; 1148.1651741; -.
DR PaxDb; P72667; -.
DR EnsemblBacteria; BAA16669; BAA16669; BAA16669.
DR KEGG; syn:slr0722; -.
DR eggNOG; COG1385; Bacteria.
DR InParanoid; P72667; -.
DR OMA; TQWKGER; -.
DR PhylomeDB; P72667; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:InterPro.
DR GO; GO:0070042; F:rRNA (uridine-N3-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR Gene3D; 3.40.1280.10; -; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR001206; Diacylglycerol_kinase_cat_dom.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR006700; rRNA_ssu_MeTrfase-E.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR PANTHER; PTHR30027; PTHR30027; 1.
DR Pfam; PF04452; Methyltrans_RNA; 1.
DR SMART; SM00046; DAGKc; 1.
DR SUPFAM; SSF75217; SSF75217; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00046; TIGR00046; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..303
FT /note="Ribosomal RNA small subunit methyltransferase E"
FT /id="PRO_0000176217"
SQ SEQUENCE 303 AA; 32775 MW; B85A4B187EED0AAB CRC64;
MLAKAFAGSK SHKILINAWF TTLPGQCQKP IVQFVLSPIL VSTNLTPSIL SLPVQEKSSS
SFVMAYRLVV APEQIIEATV TLTTAQLHYL QRVLRLQKGD GFMVLDGGGG VWRAQLNDLA
HGTAQLLETV SEQNELPLPV TLAIALPKGS GFEEIIRPCT ELGATAFQPL LTERTLLKPS
QNKLERWQRI VTEAAEQSER QWLPPVAAPL TFGQFVEKVA GPETLALLCV TRLNSPMLGA
YLKQSNLPAQ IVLATGPEGG WTDNEISLAI AKGFQPVSLG KRILRAVTAP TVALAQINAL
LES
