BCHA1_ARATH
ID BCHA1_ARATH Reviewed; 3601 AA.
AC F4HZB2; Q9SA64;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 23-FEB-2022, entry version 72.
DE RecName: Full=Protein SPIRRIG {ECO:0000303|PubMed:19392685};
DE AltName: Full=BEACH domain-containing protein A1 {ECO:0000303|PubMed:25618824};
DE AltName: Full=BEACH-domain homolog A1 {ECO:0000305};
GN Name=SPI {ECO:0000303|PubMed:19392685};
GN Synonyms=BCHA1 {ECO:0000303|PubMed:25618824};
GN OrderedLocusNames=At1g03060 {ECO:0000312|Araport:AT1G03060};
GN ORFNames=F10O3.12 {ECO:0000312|EMBL:AAD25803.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19392685; DOI=10.1111/j.1365-313x.2009.03900.x;
RA Saedler R., Jakoby M., Marin B., Galiana-Jaime E., Hulskamp M.;
RT "The cell morphogenesis gene SPIRRIG in Arabidopsis encodes a WD/BEACH
RT domain protein.";
RL Plant J. 59:612-621(2009).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=25618824; DOI=10.1016/j.molp.2014.11.015;
RA Teh O.K., Hatsugai N., Tamura K., Fuji K., Tabata R., Yamaguchi K.,
RA Shingenobu S., Yamada M., Hasebe M., Sawa S., Shimada T.,
RA Hara-Nishimura I.;
RT "BEACH-domain proteins act together in a cascade to mediate vacuolar
RT protein trafficking and disease resistance in Arabidopsis.";
RL Mol. Plant 8:389-398(2015).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH DCP1, AND SUBCELLULAR
RP LOCATION.
RX PubMed=26133670; DOI=10.1371/journal.pbio.1002188;
RA Steffens A., Braeutigam A., Jakoby M., Huelskamp M.;
RT "The BEACH domain protein SPIRRIG is essential for Arabidopsis salt stress
RT tolerance and functions as a regulator of transcript stabilization and
RT localization.";
RL PLoS Biol. 13:E1002188-E1002188(2015).
CC -!- FUNCTION: Involved in cell morphogenesis (PubMed:19392685). May have a
CC function in membrane fusion or membrane composition (PubMed:19392685).
CC Required for salt stress tolerance (PubMed:26133670). Regulates the
CC salt stress-dependent post-transcriptional stabilization, cytoplasmic
CC agglomeration, and localization to P-bodies of a subset of salt stress-
CC regulated mRNAs (PubMed:26133670). {ECO:0000269|PubMed:19392685,
CC ECO:0000269|PubMed:26133670}.
CC -!- SUBUNIT: Interacts with DCP1. {ECO:0000269|PubMed:26133670}.
CC -!- INTERACTION:
CC F4HZB2; Q9SJF3: At1g08370; NbExp=5; IntAct=EBI-3386960, EBI-7786643;
CC F4HZB2; Q12517: DCP1; Xeno; NbExp=2; IntAct=EBI-3386960, EBI-38519;
CC F4HZB2; Q9NPI6: DCP1A; Xeno; NbExp=2; IntAct=EBI-3386960, EBI-374238;
CC F4HZB2; Q8IZD4: DCP1B; Xeno; NbExp=2; IntAct=EBI-3386960, EBI-521595;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:26133670}.
CC Cytoplasm, P-body {ECO:0000269|PubMed:26133670}. Note=Recruited to P-
CC bodies by DCP1 in a salt stress-dependent manner.
CC {ECO:0000269|PubMed:26133670}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers, leaves, stems, hypocotyls and
CC roots. {ECO:0000269|PubMed:19392685}.
CC -!- DISRUPTION PHENOTYPE: Distorted trichomes, decreased lobing of
CC epidermal pavement cells, disconnected epidermal cells on various
CC organs and shorter root hairs (PubMed:19392685). Fragmented vacuoles in
CC root hairs (PubMed:19392685). Salt hypersensitivity.
CC {ECO:0000269|PubMed:19392685, ECO:0000269|PubMed:26133670}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD25803.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006550; AAD25803.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27523.1; -; Genomic_DNA.
DR PIR; D86161; D86161.
DR RefSeq; NP_001322129.1; NM_001331381.1.
DR RefSeq; NP_171805.1; NM_100188.1.
DR SMR; F4HZB2; -.
DR DIP; DIP-61571N; -.
DR IntAct; F4HZB2; 5.
DR STRING; 3702.AT1G03060.1; -.
DR iPTMnet; F4HZB2; -.
DR PaxDb; F4HZB2; -.
DR PRIDE; F4HZB2; -.
DR ProteomicsDB; 240648; -.
DR EnsemblPlants; AT1G03060.1; AT1G03060.1; AT1G03060.
DR GeneID; 839323; -.
DR Gramene; AT1G03060.1; AT1G03060.1; AT1G03060.
DR KEGG; ath:AT1G03060; -.
DR Araport; AT1G03060; -.
DR TAIR; locus:2007514; AT1G03060.
DR eggNOG; KOG1786; Eukaryota.
DR eggNOG; KOG1788; Eukaryota.
DR HOGENOM; CLU_000175_4_0_1; -.
DR InParanoid; F4HZB2; -.
DR OrthoDB; 101142at2759; -.
DR PRO; PR:F4HZB2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HZB2; baseline and differential.
DR Genevisible; F4HZB2; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0000932; C:P-body; IDA:TAIR.
DR GO; GO:0035619; C:root hair tip; IDA:TAIR.
DR GO; GO:0071472; P:cellular response to salt stress; IMP:TAIR.
DR GO; GO:0080001; P:mucilage extrusion from seed coat; IGI:TAIR.
DR GO; GO:0009825; P:multidimensional cell growth; IMP:TAIR.
DR GO; GO:0033962; P:P-body assembly; IMP:TAIR.
DR GO; GO:1902892; P:positive regulation of root hair elongation; IMP:TAIR.
DR GO; GO:1904580; P:regulation of intracellular mRNA localization; IMP:TAIR.
DR GO; GO:1903335; P:regulation of vacuolar transport; IGI:TAIR.
DR GO; GO:0010090; P:trichome morphogenesis; IMP:TAIR.
DR GO; GO:0007033; P:vacuole organization; IMP:TAIR.
DR CDD; cd06071; Beach; 1.
DR CDD; cd01201; PH_BEACH; 1.
DR Gene3D; 1.10.1540.10; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000409; BEACH_dom.
DR InterPro; IPR036372; BEACH_dom_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR023362; PH-BEACH_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF02138; Beach; 1.
DR Pfam; PF14844; PH_BEACH; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM01026; Beach; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF48371; SSF48371; 2.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81837; SSF81837; 1.
DR PROSITE; PS50197; BEACH; 1.
DR PROSITE; PS51783; PH_BEACH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..3601
FT /note="Protein SPIRRIG"
FT /evidence="ECO:0000255"
FT /id="PRO_0000434032"
FT DOMAIN 2760..2927
FT /note="BEACH-type PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01119"
FT DOMAIN 2952..3244
FT /note="BEACH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00026"
FT REPEAT 3328..3367
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 3378..3417
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 3464..3507
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 3540..3579
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REGION 17..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 398..426
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 449..476
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 638..657
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1954..1993
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2009..2049
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2715..2747
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 453..476
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1960..1993
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2024..2042
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2715..2731
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2732..2747
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3601 AA; 400894 MW; 6B3D29DA84E635B0 CRC64;
MKWATLLKDI KEKVGLAQSS DSDPFPVDLT APPSSSSSSS SPSFTYPSSS SLHHFNFSPS
SRDNHELELD FKRLWEEFRS SSSEKEKEAA LNLTVDIFCR LVKRHANVDQ LVTMLVETHI
FSFVIGRAFV TDIEKLKIGS KTRSLNVEKV LRFFSDVTKE GFSPGANLLT AVEVLVSGPI
DKQSLLDSGI FCCLIHVLIA LLAYDELSKS KITGDLEVVS AEKDAGYIVL QTRRLEVEGS
VVHIMKALAS NPSAAQSLIE DDSLESLFNM VANGSITVFS QYKEGLVPLH NIQLHRHAMQ
ILGLLLVNDN GSTARYIRKH HLIKVLLMAV KEFDPSCGDS AYTMGIVDLL LECVELSYRP
EAGGVRLRED IRNAHGYHFL VQFALVLSSL PKNPIFVSSN HDSGSDDPEV FHDGENTNST
ENADFSSQNF APSLSRLLDV LVTLAQTGPA EPSVGRASRS SQTKPTGHSR SRTSSVDSIY
DETWEQGSGK VKDLEAVQML QDIFLKAENK DLQAEVLNRM FKIFSSHVEN YRLCQELRTV
PLLVLNMAGF PSSLQDIILK ILEYAVTVVN CVPEQELLSL CCLLQQPITS QLKHTILSFF
VKLISFDQQY KKVLREVGVL EVLQDDLKQH KLLIGPDQYS GVSSHSDRKP SSGSFRKNLD
TKDAIISSPK LMESGSGKLP VFEVDNTITV GWDCLISLLK KAEANQSSFR AANGVAIILP
FLISDAHRSG VLRILSCLIT EDTKQVHHDE LGAVVDLLKS GMVTGISGHQ YKLHDDAKCD
TMGALWRIVG VNGSAQRVFG EATGFSLLLT TLHTFQGKRE HMDESDLTVY IKLFKYLFRL
MTAAVCENAV NRMKLHAVIT SQTFFELLAE SGLLCVELER QVIQLLLELA LEVVVPPFLT
SESTALATIP ENENTTFVVT TPSGQFNPDK ERIYNAGAVR VLIRSLLLFS PKMQLEFLRL
LESLARASPF NQENLTSIGC VELLLEIIYP FLAGSSPFLS YALKIVEILG AYRLSPSELR
MLFRYVLQMR IMNSGHAIVG MMEKLILMED TALEHLSLAP FVELDMSKTG HASVQVSLGE
RSWPPAAGYS FVCWFQFRNF LTTQGKESEA SKAGGSSKTR MTSAQQHEQN IFRMFSVGAV
SNESPFYAEL YFQEDGILTL ATSNSHSLSF SGLEIEEGRW HHLAVVHSKP NALAGLFQAS
VAYVYLDGKL RHTGKLGYSP SPVGKSLQVT VGTPATCARV SDLTWKTRSC YLFEEVLTSG
CIGFMYILGR GYKGLFQDAD LLRFVPNQAC GGGSMAILDS LDTDMTSSSN GQKFDGSNRQ
GDSKADGSGI VWDLERLGNL AFQLPGKKLI FAFDGTCSEF IRASGNFSLL NLVDPLSAAA
SPIGGIPRFG RLVGNVSICR QSVIGDTIRP VGGMTVVLAL VEAAESRNML HMALSLLACA
LHQNPQNVKD MQTIRGYHLL ALFLRPKMTL FDMQSLEIFF QIAACEALFS EPKKLESVQS
NITMPPTETI FENSYEDLSL SRFRYDSSSV GSHGDMDDFS VPKDSFSHLS ELETDIPVET
SNCIVLSNAD MVEHVLLDWT LWVTSPVSIQ IALLGFLENL VSMHWYRNHN LTILRRINLV
EHLLVTLQRG DVEVPVLEKL VVLLGCILED GFLTSELENV VRFVIMTFNP PEVKSRSSLL
RESMGKHVIV RNMLLEMLID LQVTIKAEDL LELWHKIVSS KLITYFLDEA VHPTSMRWIM
TLLGVCLASS PNFSLKFRTS GGYQGLLRVL QNFYDSPDIY YILFCLIFGK PVYPRLPEVR
MLDFHALVPN DGSYVELKFI ELLDSVVAMA KSTYDRLIMQ SMLAHQSGNL SQVSASLVAE
LIEGAEMTGE LQGEALMHKT YAARLMGGEA SAPAAATSVL RFMVDLAKMC PQFSTACRRA
EFVENCADLY FSCVRAAYAV KMAKQLSVKA EEKHINDADD SGSQGSLPHD QDQSTKTSIS
VGSFPQGQVS LGSEDMSLPA NYVVNDKMEN ILPPPTQDTS KSLQGVEDVK KQDDHHVGPS
ASSERDFQDF TGNPVQVQAT DSQSSASFPM IESPLLSEKS SLKVSFTPSP SPVVALASWL
GSNYNESKSS TLGSPSLESY VSVNEVDASS ERKSGSQGSS AANAFFTVSP KLLLETDETG
YGGGPCSAGA SAVLDFMAEA LADLVTEQIK AVPVLESILE MVPFYVDPES VLVFQGLCLS
RVMNYLERRL LRDDEEDEKK LDKAKWSVNL DAFCWMIVDR VYMGAFSQPA GVLRALEFLL
SMLQLANKDG RVEEVTPSGK GLLSLGRATR QLDAYVHSIL KNTNRMVLYC FLPSFLITIG
EEDLLSQLGL LVESKKRPSP NPATDESGID ISTVLQLLVA NRRIIFCPSN LDTDLNCCLC
VNLISLLLDQ RKSVQNMSLD IVKYLLVHRR SALEDLLVTK PNQGQNFDVL HGGFDKLLTG
NLPEFFKWLE SSDKIINKVL EQCAAIMWVQ YIAGSAKFPG VRIKGMEGRR KREMGRKSRD
MSKLDLKHWD QLNERRYALE VLRDAMSTEL RVVRQNKYGW ILHAESEWQT HLQQLVHERG
IFPMRKSKGT EDPEWQLCPI EGPYRMRKKL ERCKLKIDSI QNVLDGKLEL GEIELPKVKN
EDGPVISDTD SEPPFLLSEL YDESFLKESD DFKDVASARN GWNDDRASST NEASLHSALD
FGGKSSIASV PITDTTHVKS ETGSPRHSSS AKMDETNGRE EKSEKELNDD GEYLIRPYLE
HLEKIRFRYN CERVVDLDKH DGIFLIGEFC LYVIENFYID EDGCICEKEC EDELSVIDQA
LGVKKDVSGS SDFHSKSSTS WTTTVKTGAV GGRAWAYGGG AWGKEKMCMT GNLPHPWRMW
KLNNVHEILK RDYQLRPVAI EIFSMDGCND LLVFHKKERE EVFKNLVAMN LPRNSMLDTT
ISGSAKQESN EGGRLFKLMA KSFSKRWQNG EISNFQYLMH LNTLAGRGYS DLTQYPVFPW
VLADYDSESL DFSDPKTFRK LHKPMGCQTP EGEEEFRKRY ESWDDPEVPK FHYGSHYSSA
GIVLFYLIRL PPFSSENQKL QGGQFDHADR LFNSIKDTWL SAAGKGNTSD VKELIPEFFY
MPEFLENRFS LDLGEKQSGE KVGDVFLPPW ARGSVREFIL KHREALESDY VSENLHHWID
LIFGYKQRGK AAEEAVNVFY HYTYEGNVDI DAVTDPAMKA SILAQINHFG QTPKQLFPKA
HVKRRTDRKI PLHPLKHSMH LVPHEIRKCS SSISQIITFH DKVLVAGANC FLKPRGYTKY
ITWGFPDRSL RFMSYDQDKL LSTHENLHES NQIQCAGVSH DGRIVVTGAE DGLVCVWRVS
KDGPRGSRRL RLEKALCAHT AKVTCLRVSQ PYMMIASGSD DCTVIIWDLS SLSFVRQLPD
FPVPISAIYI NDLTGEIVTA AGTVLAVWSI NGDCLAVANT SQLPSDSVLS VTGSTSSDWL
ETSWYVTGHQ SGAVKVWRMI HCTDPVSAES KTSSSNRTGG LNLGDQVPEY KLILHKVLKF
HKQPVTALHL TSDLKQLLSG DSAGQLLSWT VPDETLRASM KQASLKQASL KQASLKQASS
V
