RSMG_SALTY
ID RSMG_SALTY Reviewed; 207 AA.
AC P64237; Q8XFW0;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase G {ECO:0000255|HAMAP-Rule:MF_00074};
DE EC=2.1.1.170 {ECO:0000255|HAMAP-Rule:MF_00074};
DE AltName: Full=16S rRNA 7-methylguanosine methyltransferase {ECO:0000255|HAMAP-Rule:MF_00074};
DE Short=16S rRNA m7G methyltransferase {ECO:0000255|HAMAP-Rule:MF_00074};
GN Name=rsmG {ECO:0000255|HAMAP-Rule:MF_00074}; OrderedLocusNames=STM3873;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [2]
RP MUTAGENESIS OF ASP-56.
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=21507083; DOI=10.1111/j.1365-2958.2011.07657.x;
RA Koskiniemi S., Praenting M., Gullberg E., Naesvall J., Andersson D.I.;
RT "Activation of cryptic aminoglycoside resistance in Salmonella enterica.";
RL Mol. Microbiol. 80:1464-1478(2011).
CC -!- FUNCTION: Specifically methylates the N7 position of guanine in
CC position 527 of 16S rRNA. {ECO:0000255|HAMAP-Rule:MF_00074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(527) in 16S rRNA + S-adenosyl-L-methionine = N(7)-
CC methylguanosine(527) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42732, Rhea:RHEA-COMP:10209, Rhea:RHEA-COMP:10210,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269,
CC ChEBI:CHEBI:74480; EC=2.1.1.170; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00074};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00074}.
CC -!- MISCELLANEOUS: A mutant in which deletion of the last 50 residues in
CC ubiA is combined with the Tyr-56 mutation in rsmG is highly resistant
CC to streptomycin and has a small colony phenotype (which grow slowly and
CC are resistant to aminoglycoside anitbiotics).
CC {ECO:0000269|PubMed:21507083}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RNA
CC methyltransferase RsmG family. {ECO:0000255|HAMAP-Rule:MF_00074}.
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DR EMBL; AE006468; AAL22731.1; -; Genomic_DNA.
DR RefSeq; NP_462772.3; NC_003197.2.
DR RefSeq; WP_001519938.1; NC_003197.2.
DR AlphaFoldDB; P64237; -.
DR SMR; P64237; -.
DR STRING; 99287.STM3873; -.
DR PaxDb; P64237; -.
DR EnsemblBacteria; AAL22731; AAL22731; STM3873.
DR GeneID; 1255400; -.
DR KEGG; stm:STM3873; -.
DR PATRIC; fig|99287.12.peg.4103; -.
DR HOGENOM; CLU_065341_2_2_6; -.
DR OMA; ICFPHLH; -.
DR PhylomeDB; P64237; -.
DR BioCyc; SENT99287:STM3873-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0070043; F:rRNA (guanine-N7-)-methyltransferase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_00074; 16SrRNA_methyltr_G; 1.
DR InterPro; IPR003682; rRNA_ssu_MeTfrase_G.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR31760; PTHR31760; 1.
DR Pfam; PF02527; GidB; 1.
DR PIRSF; PIRSF003078; GidB; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR00138; rsmG_gidB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..207
FT /note="Ribosomal RNA small subunit methyltransferase G"
FT /id="PRO_0000184324"
FT BINDING 73
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00074"
FT BINDING 78
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00074"
FT BINDING 124..125
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00074"
FT BINDING 139
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00074"
FT MUTAGEN 56
FT /note="D->A: Low level streptomycin and spectinomycin
FT resistance."
FT /evidence="ECO:0000269|PubMed:21507083"
SQ SEQUENCE 207 AA; 23175 MW; 788C535414CA8F15 CRC64;
MLNKLSRLLA DAGISLTDHQ KTLLVAYVDM LHKWNKAYNL TSVRDPNEML VRHILDSIVV
APYLQGQRFI DVGTGPGLPG IPLAIVLPDA HFTLLDSLGK RVRFLRQVQH ELKLENITPV
QSRVEAYPSE PPFDGVISRA FASLNDMVSW CHHLPGEKGR FYALKGQLPG DEIASLPDNF
SVESVEKLRV PQLEGERHLV IIKSNKV
