RSMG_STRCO
ID RSMG_STRCO Reviewed; 239 AA.
AC O54571; Q9RFM0;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase G {ECO:0000255|HAMAP-Rule:MF_00074};
DE EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_00074};
DE AltName: Full=16S rRNA 7-methylguanosine methyltransferase {ECO:0000255|HAMAP-Rule:MF_00074};
DE Short=16S rRNA m7G methyltransferase {ECO:0000255|HAMAP-Rule:MF_00074};
DE AltName: Full=Glucose-inhibited division protein B;
GN Name=rsmG {ECO:0000255|HAMAP-Rule:MF_00074}; Synonyms=gidB;
GN OrderedLocusNames=SCO3885; ORFNames=STH24.07;
OS Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces; Streptomyces albidoflavus group.
OX NCBI_TaxID=100226;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RA Gal-Mor O., Borovok I., Av-Gay Y., Cohen G., Aharonowitz Y.;
RT "Gene organization in the trxB/A-oriC region of the Streptomyces coelicolor
RT 3A(2) chromosome and comparison with other bacteria.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=A3(2) / NRRL B-16638;
RX PubMed=10671452; DOI=10.1128/jb.182.5.1313-1320.2000;
RA Kim H.-J., Calcutt M.J., Schmidt F.J., Chater K.F.;
RT "Partitioning of the linear chromosome during sporulation of Streptomyces
RT coelicolor A3(2) involves an oriC-linked parAB locus.";
RL J. Bacteriol. 182:1313-1320(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=12000953; DOI=10.1038/417141a;
RA Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT A3(2).";
RL Nature 417:141-147(2002).
RN [4]
RP FUNCTION AS A M7G METHYLTRANSFERASE, AND DISRUPTION PHENOTYPE.
RC STRAIN=A3(2) / 1147;
RX PubMed=17384192; DOI=10.1128/jb.01776-06;
RA Nishimura K., Hosaka T., Tokuyama S., Okamoto S., Ochi K.;
RT "Mutations in rsmG, encoding a 16S rRNA methyltransferase, result in low-
RT level streptomycin resistance and antibiotic overproduction in Streptomyces
RT coelicolor A3(2).";
RL J. Bacteriol. 189:3876-3883(2007).
CC -!- FUNCTION: Specifically methylates the N7 position of guanine in
CC position 518 of 16S rRNA. {ECO:0000255|HAMAP-Rule:MF_00074,
CC ECO:0000269|PubMed:17384192}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00074}.
CC -!- DISRUPTION PHENOTYPE: Confers low-level streptomycin resistance and a
CC greater ability to produce the antibiotic actinorhodin.
CC {ECO:0000269|PubMed:17384192}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RNA
CC methyltransferase RsmG family. {ECO:0000255|HAMAP-Rule:MF_00074}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF16006.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF031590; AAC03485.1; -; Genomic_DNA.
DR EMBL; AF187159; AAF16006.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL939118; CAB42703.1; -; Genomic_DNA.
DR PIR; T36572; T36572.
DR RefSeq; NP_628071.1; NC_003888.3.
DR RefSeq; WP_011029291.1; NZ_VNID01000003.1.
DR AlphaFoldDB; O54571; -.
DR SMR; O54571; -.
DR STRING; 100226.SCO3885; -.
DR GeneID; 1099321; -.
DR KEGG; sco:SCO3885; -.
DR PATRIC; fig|100226.15.peg.3958; -.
DR eggNOG; COG0357; Bacteria.
DR HOGENOM; CLU_065341_5_0_11; -.
DR InParanoid; O54571; -.
DR OMA; WDRHVLN; -.
DR PhylomeDB; O54571; -.
DR BRENDA; 2.1.1.170; 5998.
DR Proteomes; UP000001973; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0070043; F:rRNA (guanine-N7-)-methyltransferase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_00074; 16SrRNA_methyltr_G; 1.
DR InterPro; IPR003682; rRNA_ssu_MeTfrase_G.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR31760; PTHR31760; 1.
DR Pfam; PF02527; GidB; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR00138; rsmG_gidB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..239
FT /note="Ribosomal RNA small subunit methyltransferase G"
FT /id="PRO_0000184349"
FT BINDING 75
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00074"
FT BINDING 80
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00074"
FT BINDING 126..127
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00074"
FT BINDING 142
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00074"
SQ SEQUENCE 239 AA; 25647 MW; 2297957AB2C8C011 CRC64;
MSEAAELPPV PEQARDVFGD RYADAVRYAE LLAEAGVKRG LIGPREVPRL WERHLLNCAV
LSEVVPEGVT VCDVGSGAGL PGIPLALVRE DLKITLLEPL LRRTNFLTEV VELLGLDHVT
VVRGRAEEVM GKLPPVHVVT ARAVAPLDRL ATWGIPLLRP YGEMLALKGD TAEEELKAAT
AALSKLGAEQ TSILHVGEGV VSPLSTVVRV EVGESPGGVR FAAKRAKAAR TGRTRRRRG
