BCHC1_ARATH
ID BCHC1_ARATH Reviewed; 1941 AA.
AC F4JD14; Q9LEX9;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=BEACH domain-containing protein C1 {ECO:0000303|PubMed:25618824};
DE AltName: Full=BEACH-domain homolog C1 {ECO:0000305};
GN Name=BCHC1 {ECO:0000303|PubMed:25618824};
GN OrderedLocusNames=At3g60920 {ECO:0000312|Araport:AT3G60920};
GN ORFNames=T27I15.10 {ECO:0000312|EMBL:CAB94128.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000312|Proteomes:UP000006548};
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia {ECO:0000312|Proteomes:UP000006548};
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, GENE FAMILY, NOMENCLATURE, DISRUPTION PHENOTYPE, INTERACTION WITH
RP GFS12, DOMAIN, AND MUTAGENESIS OF ARG-1616; GLU-1626; ASN-1705 AND
RP GLN-1709.
RX PubMed=25618824; DOI=10.1016/j.molp.2014.11.015;
RA Teh O.K., Hatsugai N., Tamura K., Fuji K., Tabata R., Yamaguchi K.,
RA Shingenobu S., Yamada M., Hasebe M., Sawa S., Shimada T.,
RA Hara-Nishimura I.;
RT "BEACH-domain proteins act together in a cascade to mediate vacuolar
RT protein trafficking and disease resistance in Arabidopsis.";
RL Mol. Plant 8:389-398(2015).
CC -!- FUNCTION: May act as a negative factor in protein storage vacuole (PSV)
CC trafficking regulation and plant effector triggered immunity (ETI).
CC Negatively regulated upon interaction with GSF12, and also probably by
CC BCHB. Required for ETI, but not for cell death.
CC {ECO:0000269|PubMed:25618824}.
CC -!- SUBUNIT: Interacts (via BEACH-type PH domain) with GFS12 (via protein
CC kinase 2 domain). {ECO:0000269|PubMed:25618824}.
CC -!- DOMAIN: The BEACH-type PH domain (1556-1653) is required for
CC interaction with GFS12. {ECO:0000269|PubMed:25618824}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:25618824}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB94128.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL358732; CAB94128.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE80127.1; -; Genomic_DNA.
DR PIR; T50513; T50513.
DR RefSeq; NP_191651.3; NM_115956.3.
DR AlphaFoldDB; F4JD14; -.
DR SMR; F4JD14; -.
DR STRING; 3702.AT3G60920.1; -.
DR iPTMnet; F4JD14; -.
DR PaxDb; F4JD14; -.
DR PRIDE; F4JD14; -.
DR ProteomicsDB; 240853; -.
DR EnsemblPlants; AT3G60920.1; AT3G60920.1; AT3G60920.
DR GeneID; 825263; -.
DR Gramene; AT3G60920.1; AT3G60920.1; AT3G60920.
DR KEGG; ath:AT3G60920; -.
DR Araport; AT3G60920; -.
DR TAIR; locus:2100942; AT3G60920.
DR eggNOG; KOG1787; Eukaryota.
DR HOGENOM; CLU_000307_0_0_1; -.
DR InParanoid; F4JD14; -.
DR PRO; PR:F4JD14; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4JD14; baseline and differential.
DR Genevisible; F4JD14; AT.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR CDD; cd06071; Beach; 1.
DR CDD; cd01201; PH_BEACH; 1.
DR Gene3D; 1.10.1540.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR000409; BEACH_dom.
DR InterPro; IPR036372; BEACH_dom_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR031570; NBEA/BDCP_DUF4704.
DR InterPro; IPR023362; PH-BEACH_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR Pfam; PF02138; Beach; 1.
DR Pfam; PF15787; DUF4704; 1.
DR Pfam; PF14844; PH_BEACH; 1.
DR SMART; SM01026; Beach; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF81837; SSF81837; 1.
DR PROSITE; PS50197; BEACH; 1.
DR PROSITE; PS51783; PH_BEACH; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..1941
FT /note="BEACH domain-containing protein C1"
FT /id="PRO_0000434035"
FT DOMAIN 1544..1653
FT /note="BEACH-type PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01119"
FT DOMAIN 1667..1941
FT /note="BEACH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00026"
FT REGION 1481..1513
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1829..1923
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1490..1511
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1829..1922
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 1616
FT /note="R->E: Loss of interaction with GFS12."
FT /evidence="ECO:0000269|PubMed:25618824"
FT MUTAGEN 1626
FT /note="E->R: Loss of interaction with GFS12."
FT /evidence="ECO:0000269|PubMed:25618824"
FT MUTAGEN 1705
FT /note="N->A: No effec on interaction with GFS12."
FT /evidence="ECO:0000269|PubMed:25618824"
FT MUTAGEN 1709
FT /note="Q->A: No effec on interaction with GFS12."
FT /evidence="ECO:0000269|PubMed:25618824"
SQ SEQUENCE 1941 AA; 213254 MW; CDDB176A27DEF901 CRC64;
MNGKESRGPA CSFEFVGESS GLLGPGESRW PFTNGYAFAT WIYIESFADT LDASTAAAAI
AAASAAKSGK ISNAAPANVH TGEGTAHMPR LFSFLTPDNQ GIEAYFYAQF LVVESGSGKG
SKTSLHFTHA FKPQCWYFIG LEHTCNQGLL GNSDSELRLY IDGSLYETRP FDYPRISKPL
SFCCIGSNPP STTAGLQRRR RQCALFAEMG PVYIFKEPIG PERMTRLATR GGDVLPCFGN
GAGLPWLATN DHVRNVAEES SLSDAELGGY IHLLYHPCLL SGRFCPDASL SGAAGDEKTP
YSFSAVVFAL CYLVFPYFGR PMSLLPLTVS SVHKDNLEPC SRNVPSSLTT YSLAAPIFRM
ISFAIKHPGN NEELSRTRGP EILATVLGYL LHSLASFDIK HDRVGDEELV AAIVSLCQSQ
KINHALKVQL FCTLLLDLKI WSVCSYRLQK KLLSSLQDMV FTEATAMRNA DAIQVLLDGC
RRYFWTIQEK DSVNTFSLDG DARQVGEVNA LVDELLVIIE LLMGAASPSF AADDLHRLLG
FIIESPQPNQ VARVLHLMFR LVVQPNAAKA QTFAEAFITS GGIETLLVLI ANRVNTMGLE
TESFQQVKVH GSETVICETG SVTLSSSVNA DRISSVSETP FNNNARNNVD DRDRVMVGII
RLIGALISKG HLKFSVGAKS DVMSNLMGSE FRENGGTMFD YKVALLLFAL LKAFQAAPNR
LMTDNVYTTL LGASVNASST EDGLNFCDLG HRFEHPQLLL ILLRSLPFAS KALQNRALQD
ILFLACTHPE KRSSLTKMEE WPEWILEILI SNYEKDAGKQ SASPGSAEEE DLIHNFLIIM
LEHSMRQKDG WKDIEATIHC AEWLTFVGGS STGEKRIRRE ESLPIFKRRL LGGLLDFAAS
ELQAQTQVIA AASAGFAAES LTPKDAKAGV ENAALLSVFL VENTVVILML VEDHLRLQSK
QNCAASAVDV SPSPLSLVYP PNYRSHTLPT VGESSEVSSS CASVSSDSGG VHLDILASMA
DASGQISTAV MERLAAAAAA EPYESVSCAF VSYGSCTRDL ADAWKYRSRL WYGVGLPSKT
SCFGGGGSGW DSWKHALQKD AQGNWIELPL VKKSVSMLQA LLLDESGLGG GLGIGGGSGT
GMGGMSGLYQ LLDSDQPFLC MLRMVLLSLR EEDHGEDSLL MKNLSSEDGI TGGLQCPLGN
SASVDISSQL SMRQSPSALL WSVLSPVLNM PISDSKRQRV LVTTCVLYSE VWNAVSKDKR
PLRKQYLEAI LPPFVAILRR WRPLLAGIHE LSTGDGVNPL VVDTRALAAD ALPIEAALSM
ISPEWAAAFA SPPSAMALAM IAAGAAGWEA PAHPAPPAPP PLRRDSSLLE RKSTKLQTFS
SFQKPLEAPN DDTPGRARDK AAANIDSHLL IQPLTRLTGV FWKLDSMESS SRMRQCLRRN
YSGTGHLETT RNYGDQTYLM NNHDSPVLAV EAISKEIMYE DDEHGDADDL EIEGNVGERK
GENEERRSGS LEDAITLSTG INDHRPLSEQ NMVQNSTEVK DLSELKERIV LEISSTMVRP
LGVVKGTFQI TTRRINFIVD IREDQHLDEK SDGSKSRDEE RDRSWLMSSL HQIYSRRYLL
KKSALELFMV DRSNFFFDFG NTEGRRNACR AIVQARPPHL KNIYSATQPE QVSRRTQLME
RWARWEISNF EYLMQLNTLA GRSYNDITQY PIFPWILCDY VSEILDLSNP SNYRDLSKVP
IGALNPERLK KFQEKHSSFE DPVIPKFHYG SHYSSAGAVL HYLARVEPFT TLSIQLQGRK
FDRADQIFSD IAATWKGVLQ DMNNVKELNT TDPNTHMVSS FSNPTTSETE PDSDMNIVST
PSNATTNQID TESSEAANYE NSNSSIKTSK NTSKITKLTP TSKRSLTSSK DNAAQKSSTK
PKLLSKAEII KVDVYSYIMS L
