BCHI_CERS4
ID BCHI_CERS4 Reviewed; 334 AA.
AC O30819; Q3J188; Q9RFD2;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Magnesium-chelatase 38 kDa subunit;
DE EC=6.6.1.1;
DE AltName: Full=Mg-protoporphyrin IX chelatase;
GN Name=bchI; OrderedLocusNames=RHOS4_18780; ORFNames=RSP_0273;
OS Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=272943;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Hansson M., Kannangara C.G.;
RT "Rhodobacter sphaeroides bchI and bchD encoding two subunits of magnesium
RT chelatase.";
RL (er) Plant Gene Register PGR97-190(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10648776; DOI=10.1093/nar/28.4.862;
RA Choudhary M., Kaplan S.;
RT "DNA sequence analysis of the photosynthesis region of Rhodobacter
RT sphaeroides 2.4.1.";
RL Nucleic Acids Res. 28:862-867(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC / NCIMB 8253 / ATH 2.4.1.;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP CHARACTERIZATION.
RX PubMed=7892204; DOI=10.1073/pnas.92.6.1941;
RA Gibson L.C.D., Willows R.D., Kannangara C.G., von Wettstein D.,
RA Hunter C.N.;
RT "Magnesium-protoporphyrin chelatase of Rhodobacter sphaeroides:
RT reconstitution of activity by combining the products of the bchH, -I, and
RT -D genes expressed in Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:1941-1944(1995).
CC -!- FUNCTION: Involved in bacteriochlorophyll biosynthesis; introduces a
CC magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Mg(2+) + protoporphyrin IX = ADP + 3 H(+) + Mg-
CC protoporphyrin IX + phosphate; Xref=Rhea:RHEA:13961,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57306,
CC ChEBI:CHEBI:60492, ChEBI:CHEBI:456216; EC=6.6.1.1;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; bacteriochlorophyll
CC biosynthesis.
CC -!- SIMILARITY: Belongs to the Mg-chelatase subunits D/I family.
CC {ECO:0000305}.
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DR EMBL; AF017642; AAB97156.1; -; Genomic_DNA.
DR EMBL; AF195122; AAF24287.1; -; Genomic_DNA.
DR EMBL; CP000143; ABA79446.1; -; Genomic_DNA.
DR PIR; T50743; T50743.
DR RefSeq; WP_002720437.1; NZ_CP030271.1.
DR RefSeq; YP_353347.1; NC_007493.2.
DR AlphaFoldDB; O30819; -.
DR SMR; O30819; -.
DR STRING; 272943.RSP_0273; -.
DR EnsemblBacteria; ABA79446; ABA79446; RSP_0273.
DR GeneID; 67447006; -.
DR KEGG; rsp:RSP_0273; -.
DR PATRIC; fig|272943.9.peg.2216; -.
DR eggNOG; COG1239; Bacteria.
DR OMA; RISAVCG; -.
DR PhylomeDB; O30819; -.
DR BioCyc; MetaCyc:MON-13263; -.
DR UniPathway; UPA00669; -.
DR Proteomes; UP000002703; Chromosome 1.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016851; F:magnesium chelatase activity; IEA:UniProtKB-EC.
DR GO; GO:0030494; P:bacteriochlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR045006; CHLI-like.
DR InterPro; IPR041628; ChlI/MoxR_AAA_lid.
DR InterPro; IPR011775; Mg_chelatase_ATPase-isu.
DR InterPro; IPR000523; Mg_chelatse_chII-like_cat_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR32039; PTHR32039; 1.
DR Pfam; PF17863; AAA_lid_2; 1.
DR Pfam; PF01078; Mg_chelatase; 2.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02030; BchI-ChlI; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Bacteriochlorophyll biosynthesis; Chlorophyll biosynthesis;
KW Ligase; Nucleotide-binding; Photosynthesis; Reference proteome.
FT CHAIN 1..334
FT /note="Magnesium-chelatase 38 kDa subunit"
FT /id="PRO_0000206860"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 109
FT /note="E -> Q (in Ref. 2; AAF24287)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 334 AA; 36225 MW; 991CD08C9847E2E8 CRC64;
MKKPFPFSAI VGQEQMKQAM VLTAIDPGIG GVLVFGDRGT GKSTAVRALA ALLPLIKAVE
GCPVNSARPE DCPEWAHVSS TTMIERPTPV VDLPLGVTED RVVGALDIER ALTRGEKAFE
PGLLARANRG YLYIDEVNLL EDHIVDLLLD VAQSGENVVE REGLSIRHPA RFVLVGSGNP
EEGELRPQLL DRFGLSVEVR SPRDVETRVE VITRRDAYDA DHDAFMEKWG AEDMQLRGRI
LGARAALPQL KTPNTVLHDC AALCIALGSD GLRGELTLLR AARAQAAFEG AEAVGRSHLR
SVATMALSHR LRRDPLDEAG SVSRVERCVA EVLP
