BCHI_RHOCB
ID BCHI_RHOCB Reviewed; 350 AA.
AC P26239; D5ANT8;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Magnesium-chelatase 38 kDa subunit;
DE EC=6.6.1.1;
DE AltName: Full=Mg-protoporphyrin IX chelatase;
GN Name=bchI; OrderedLocusNames=RCAP_rcc00677;
OS Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Rhodobacter.
OX NCBI_TaxID=272942;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RA Burke D.H., Alberti M., Armstrong G.A., Hearst J.E.;
RL Submitted (NOV-1991) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=2747617; DOI=10.1007/bf00334364;
RA Armstrong G.A., Alberti M., Leach F., Hearst J.E.;
RT "Nucleotide sequence, organization, and nature of the protein products of
RT the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus.";
RL Mol. Gen. Genet. 216:254-268(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=20418398; DOI=10.1128/jb.00366-10;
RA Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA Haselkorn R.;
RT "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT Rhodobacter capsulatus SB 1003.";
RL J. Bacteriol. 192:3545-3546(2010).
CC -!- FUNCTION: Involved in bacteriochlorophyll biosynthesis; introduces a
CC magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Mg(2+) + protoporphyrin IX = ADP + 3 H(+) + Mg-
CC protoporphyrin IX + phosphate; Xref=Rhea:RHEA:13961,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57306,
CC ChEBI:CHEBI:60492, ChEBI:CHEBI:456216; EC=6.6.1.1;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; bacteriochlorophyll
CC biosynthesis.
CC -!- INTERACTION:
CC P26239; P26175: bchD; NbExp=5; IntAct=EBI-8453273, EBI-8453255;
CC -!- SIMILARITY: Belongs to the Mg-chelatase subunits D/I family.
CC {ECO:0000305}.
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DR EMBL; Z11165; CAA77538.1; -; Genomic_DNA.
DR EMBL; CP001312; ADE84442.1; -; Genomic_DNA.
DR RefSeq; WP_013066421.1; NC_014034.1.
DR PDB; 1G8P; X-ray; 2.10 A; A=1-350.
DR PDB; 2X31; EM; 7.50 A; G/H/I/J/K/L=1-350.
DR PDBsum; 1G8P; -.
DR PDBsum; 2X31; -.
DR AlphaFoldDB; P26239; -.
DR SMR; P26239; -.
DR DIP; DIP-58976N; -.
DR IntAct; P26239; 1.
DR MINT; P26239; -.
DR STRING; 272942.RCAP_rcc00677; -.
DR EnsemblBacteria; ADE84442; ADE84442; RCAP_rcc00677.
DR GeneID; 31489623; -.
DR KEGG; rcp:RCAP_rcc00677; -.
DR eggNOG; COG1239; Bacteria.
DR HOGENOM; CLU_016684_0_2_5; -.
DR OMA; RISAVCG; -.
DR OrthoDB; 895310at2; -.
DR BRENDA; 6.6.1.1; 5381.
DR UniPathway; UPA00669; -.
DR EvolutionaryTrace; P26239; -.
DR Proteomes; UP000002361; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016851; F:magnesium chelatase activity; IEA:UniProtKB-EC.
DR GO; GO:0030494; P:bacteriochlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR045006; CHLI-like.
DR InterPro; IPR041628; ChlI/MoxR_AAA_lid.
DR InterPro; IPR011775; Mg_chelatase_ATPase-isu.
DR InterPro; IPR000523; Mg_chelatse_chII-like_cat_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR32039; PTHR32039; 1.
DR Pfam; PF17863; AAA_lid_2; 1.
DR Pfam; PF01078; Mg_chelatase; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02030; BchI-ChlI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Bacteriochlorophyll biosynthesis;
KW Chlorophyll biosynthesis; Ligase; Nucleotide-binding; Photosynthesis;
KW Reference proteome.
FT CHAIN 1..350
FT /note="Magnesium-chelatase 38 kDa subunit"
FT /id="PRO_0000206859"
FT BINDING 52..59
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT HELIX 23..25
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 30..41
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 43..45
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 48..51
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 54..56
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 60..68
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 72..75
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 99..102
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 106..109
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 115..119
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 124..130
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 132..134
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 139..143
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 146..150
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 153..155
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 158..170
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 171..175
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 182..185
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 188..194
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 203..206
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 210..214
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 221..236
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 238..262
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 263..265
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 270..282
FT /evidence="ECO:0007829|PDB:1G8P"
FT STRAND 283..285
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 288..304
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 312..323
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 324..326
FT /evidence="ECO:0007829|PDB:1G8P"
FT HELIX 342..348
FT /evidence="ECO:0007829|PDB:1G8P"
SQ SEQUENCE 350 AA; 37899 MW; 5CBAA54A1F308568 CRC64;
MTTAVARLQP SASGAKTRPV FPFSAIVGQE DMKLALLLTA VDPGIGGVLV FGDRGTGKST
AVRALAALLP EIEAVEGCPV SSPNVEMIPD WATVLSTNVI RKPTPVVDLP LGVSEDRVVG
ALDIERAISK GEKAFEPGLL ARANRGYLYI DECNLLEDHI VDLLLDVAQS GENVVERDGL
SIRHPARFVL VGSGNPEEGD LRPQLLDRFG LSVEVLSPRD VETRVEVIRR RDTYDADPKA
FLEEWRPKDM DIRNQILEAR ERLPKVEAPN TALYDCAALC IALGSDGLRG ELTLLRSARA
LAALEGATAV GRDHLKRVAT MALSHRLRRD PLDEAGSTAR VARTVEETLP
