RSMH_MESCH
ID RSMH_MESCH Reviewed; 296 AA.
AC C5J697;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase H {ECO:0000255|HAMAP-Rule:MF_01007};
DE EC=2.1.1.199 {ECO:0000255|HAMAP-Rule:MF_01007};
DE AltName: Full=16S rRNA m(4)C1402 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01007};
DE AltName: Full=rRNA (cytosine-N(4)-)-methyltransferase RsmH {ECO:0000255|HAMAP-Rule:MF_01007};
GN Name=rsmH {ECO:0000255|HAMAP-Rule:MF_01007}; Synonyms=mraW;
GN OrderedLocusNames=MCJ_002980;
OS Mesomycoplasma conjunctivae (strain ATCC 25834 / NCTC 10147 / HRC/581)
OS (Mycoplasma conjunctivae).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mesomycoplasma.
OX NCBI_TaxID=572263;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25834 / NCTC 10147 / HRC/581;
RX PubMed=19534756; DOI=10.1186/1471-2105-10-s6-s7;
RA Calderon-Copete S.P., Wigger G., Wunderlin C., Schmidheini T., Frey J.,
RA Quail M.A., Falquet L.;
RT "The Mycoplasma conjunctivae genome sequencing, annotation and analysis.";
RL BMC Bioinformatics 10:S7-S7(2009).
CC -!- FUNCTION: Specifically methylates the N4 position of cytidine in
CC position 1402 (C1402) of 16S rRNA. {ECO:0000255|HAMAP-Rule:MF_01007}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) +
CC N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42928, Rhea:RHEA-COMP:10286, Rhea:RHEA-COMP:10287,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74506, ChEBI:CHEBI:82748; EC=2.1.1.199;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01007};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01007}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmH family.
CC {ECO:0000255|HAMAP-Rule:MF_01007}.
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DR EMBL; FM864216; CAT04989.1; -; Genomic_DNA.
DR AlphaFoldDB; C5J697; -.
DR SMR; C5J697; -.
DR EnsemblBacteria; CAT04989; CAT04989; MCJ_002980.
DR KEGG; mco:MCJ_002980; -.
DR eggNOG; COG0275; Bacteria.
DR HOGENOM; CLU_038422_2_0_14; -.
DR OMA; NPAKRTF; -.
DR Proteomes; UP000001491; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0071424; F:rRNA (cytosine-N4-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0070475; P:rRNA base methylation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.170; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_01007; 16SrRNA_methyltr_H; 1.
DR InterPro; IPR002903; RsmH.
DR InterPro; IPR023397; SAM-dep_MeTrfase_MraW_recog.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR11265; PTHR11265; 1.
DR Pfam; PF01795; Methyltransf_5; 1.
DR PIRSF; PIRSF004486; MraW; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR SUPFAM; SSF81799; SSF81799; 1.
DR TIGRFAMs; TIGR00006; TIGR00006; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..296
FT /note="Ribosomal RNA small subunit methyltransferase H"
FT /id="PRO_0000387001"
FT BINDING 30..32
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01007"
FT BINDING 49
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01007"
FT BINDING 97
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01007"
FT BINDING 104
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01007"
SQ SEQUENCE 296 AA; 33780 MW; 9465FCA056CBBB91 CRC64;
MHIPVLIDSL IENLNIREDG IYVDLTLGRG GHAAAILSKL TTGLLIVFDK DEKAIEESKE
RLLAISKNVI FIWEDFRNFA VELEKRQIYK VDGFIMDLGV SSPQIDQGER GFSYTKNARL
DMRMNQNQEL DAHHVVNNYN QDLLTKVLQN YGELKNARSL TKAIIDSRPI NTTFELVNLI
RSSSPAALLR KKNIVKNVFQ AIRIEVNDEL GALEAFLSLF ISYLKQDSQV AIITFHSLED
RIVKMKFKSL LISSKTHLFD PRAQQFSVKT IKPSTSEIQL NKRSKSAKLR ILSKNY
