RSP14_CHLRE
ID RSP14_CHLRE Reviewed; 387 AA.
AC A8HNV0;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=Radial spoke protein 14;
DE AltName: Full=Flagellar-associated protein 132 {ECO:0000303|PubMed:15998802};
GN Name=RSP14; Synonyms=FAP132 {ECO:0000303|PubMed:15998802};
GN ORFNames=CHLREDRAFT_172252 {ECO:0000312|EMBL:EDP10020.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=15998802; DOI=10.1083/jcb.200504008;
RA Pazour G.J., Agrin N., Leszyk J., Witman G.B.;
RT "Proteomic analysis of a eukaryotic cilium.";
RL J. Cell Biol. 170:103-113(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=16507594; DOI=10.1242/jcs.02811;
RA Yang P., Diener D.R., Yang C., Kohno T., Pazour G.J., Dienes J.M.,
RA Agrin N.S., King S.M., Sale W.S., Kamiya R., Rosenbaum J.L., Witman G.B.;
RT "Radial spoke proteins of Chlamydomonas flagella.";
RL J. Cell Sci. 6:1165-1174(2006).
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm,
CC cytoskeleton, flagellum axoneme {ECO:0000269|PubMed:16507594}.
CC Note=Radial spoke. {ECO:0000269|PubMed:16507594}.
CC -!- SIMILARITY: Belongs to the flagellar radial spoke RSP14 family.
CC {ECO:0000305}.
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DR EMBL; DS496108; EDP10020.1; -; Genomic_DNA.
DR RefSeq; XP_001690282.1; XM_001690230.1.
DR PDB; 7JTK; EM; 3.20 A; X=1-387.
DR PDBsum; 7JTK; -.
DR AlphaFoldDB; A8HNV0; -.
DR SMR; A8HNV0; -.
DR STRING; 3055.EDP10020; -.
DR PaxDb; A8HNV0; -.
DR EnsemblPlants; PNW73066; PNW73066; CHLRE_14g617500v5.
DR GeneID; 5715835; -.
DR Gramene; PNW73066; PNW73066; CHLRE_14g617500v5.
DR KEGG; cre:CHLRE_14g617500v5; -.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_714469_0_0_1; -.
DR InParanoid; A8HNV0; -.
DR OMA; DYLHDPE; -.
DR OrthoDB; 1448677at2759; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR042856; RSP14.
DR PANTHER; PTHR15599:SF1; PTHR15599:SF1; 1.
DR SMART; SM00185; ARM; 6.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW Repeat.
FT CHAIN 1..387
FT /note="Radial spoke protein 14"
FT /id="PRO_0000432960"
FT REPEAT 24..67
FT /note="ARM 1"
FT /evidence="ECO:0000255"
FT REPEAT 69..109
FT /note="ARM 2"
FT /evidence="ECO:0000255"
FT REPEAT 111..150
FT /note="ARM 3"
FT /evidence="ECO:0000255"
FT REPEAT 154..198
FT /note="ARM 4"
FT /evidence="ECO:0000255"
FT REPEAT 204..244
FT /note="ARM 5"
FT /evidence="ECO:0000255"
FT REPEAT 245..286
FT /note="ARM 6"
FT /evidence="ECO:0000255"
FT REPEAT 289..328
FT /note="ARM 7"
FT /evidence="ECO:0000255"
FT REPEAT 330..370
FT /note="ARM 8"
FT /evidence="ECO:0000255"
FT HELIX 3..16
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 39..46
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 52..65
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 69..77
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 81..89
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 94..107
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 111..119
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 122..129
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 133..135
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 136..148
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 152..159
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 161..163
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 166..178
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 183..197
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 199..201
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 203..213
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 215..221
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 229..242
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 246..255
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 258..263
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 271..284
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 288..296
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 300..307
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 310..312
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 313..326
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 332..338
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 341..351
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 355..368
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 372..374
FT /evidence="ECO:0007829|PDB:7JTK"
SQ SEQUENCE 387 AA; 40552 MW; 61D0F14D2EABBE9E CRC64;
MDSQARIQAN LRHIQAFEAK PNPKALPETA VTRGFEKVAF PKLVRELTCD NAVVRKKSLL
AARELLSSPV NHVQCVAAGA TPAIVALLQD QTDDETRYYA AGTLKLLAAK EVGARDLAQH
SGLDALAAAL EDPSEGVRDE AYGALIEAAR FDSTRRALEA CGSGAVLPRL MELALLEAQG
GAAGRAQQGL VLLFTCTQAR HNAGILSQLV DVAQAIPHLA GLLKPELPMP VRHAAAELLG
ALATREDAKI QAVQVGAVPL LLLAASPSVP VPFATSAVAA LGAITIRREG KYAALESPGG
LAGLVSVLDP CHEQLCINAM TAVSNVAEAP EARAILVASG AGPKLQHIFE TATVEVVKRA
AAQAIRQCRF KHLPYEVLPG APPINEE
