RSP1_CHLRE
ID RSP1_CHLRE Reviewed; 814 AA.
AC Q27YU0;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Flagellar radial spoke protein 1 {ECO:0000303|PubMed:16507594};
GN Name=RSP1 {ECO:0000303|PubMed:16507594};
GN ORFNames=CHLREDRAFT_196412 {ECO:0000312|EMBL:EDP03379.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055 {ECO:0000312|EMBL:ABC02025.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY,
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16507594; DOI=10.1242/jcs.02811;
RA Yang P., Diener D.R., Yang C., Kohno T., Pazour G.J., Dienes J.M.,
RA Agrin N.S., King S.M., Sale W.S., Kamiya R., Rosenbaum J.L., Witman G.B.;
RT "Radial spoke proteins of Chlamydomonas flagella.";
RL J. Cell Sci. 6:1165-1174(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503, and cw92;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [3]
RP METHYLATION AT ARG-243 AND ARG-428.
RX PubMed=24152136; DOI=10.1021/bi4011623;
RA Werner-Peterson R., Sloboda R.D.;
RT "Methylation of structural components of the axoneme occurs during
RT flagellar disassembly.";
RL Biochemistry 52:8501-8509(2013).
CC -!- FUNCTION: Flagellar radial spokes contribute to the regulation of
CC dynein arm activity and thus the pattern of flagellar bending. They
CC consist of a thin stalk, which is attached to the a subfiber of the
CC outer doublet microtubule, and a bulbous head, which is attached to the
CC stalk and appears to interact with the projections from the central
CC pair of microtubules. {ECO:0000269|PubMed:16507594}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC {ECO:0000269|PubMed:16507594}. Note=Radial spoke.
CC {ECO:0000269|PubMed:16507594}.
CC -!- PTM: Asymmetrically dimethylated at Arg-243 and Arg-428 during
CC flagellum resorption. Probably methylated by PRMT1.
CC {ECO:0000269|PubMed:24152136}.
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DR EMBL; DQ298254; ABC02025.1; -; mRNA.
DR EMBL; DS496126; EDP03379.1; -; Genomic_DNA.
DR RefSeq; XP_001693353.1; XM_001693301.1.
DR PDB; 7JRJ; EM; 3.03 A; F=1-814.
DR PDB; 7JTK; EM; 3.20 A; A/B=1-814.
DR PDBsum; 7JRJ; -.
DR PDBsum; 7JTK; -.
DR AlphaFoldDB; Q27YU0; -.
DR SMR; Q27YU0; -.
DR STRING; 3055.EDP03379; -.
DR iPTMnet; Q27YU0; -.
DR PaxDb; Q27YU0; -.
DR PRIDE; Q27YU0; -.
DR ProMEX; Q27YU0; -.
DR EnsemblPlants; PNW85872; PNW85872; CHLRE_03g201900v5.
DR GeneID; 5718906; -.
DR Gramene; PNW85872; PNW85872; CHLRE_03g201900v5.
DR KEGG; cre:CHLRE_03g201900v5; -.
DR eggNOG; KOG0231; Eukaryota.
DR HOGENOM; CLU_346963_0_0_1; -.
DR InParanoid; Q27YU0; -.
DR OMA; CAYGIPP; -.
DR OrthoDB; 1309439at2759; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProt.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR InterPro; IPR003409; MORN.
DR Pfam; PF02493; MORN; 6.
DR SMART; SM00698; MORN; 6.
PE 1: Evidence at protein level;
KW 3D-structure; Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW Methylation; Repeat.
FT CHAIN 1..814
FT /note="Flagellar radial spoke protein 1"
FT /id="PRO_0000431952"
FT REPEAT 577..597
FT /note="MORN 1"
FT /evidence="ECO:0000255"
FT REPEAT 600..622
FT /note="MORN 2"
FT /evidence="ECO:0000255"
FT REPEAT 623..645
FT /note="MORN 3"
FT /evidence="ECO:0000255"
FT REPEAT 646..662
FT /note="MORN 4"
FT /evidence="ECO:0000255"
FT REPEAT 671..685
FT /note="MORN 5"
FT /evidence="ECO:0000255"
FT REPEAT 691..707
FT /note="MORN 6"
FT /evidence="ECO:0000255"
FT REGION 283..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 739..769
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 305..329
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 243
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0000269|PubMed:24152136"
FT MOD_RES 428
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0000269|PubMed:24152136"
FT STRAND 15..19
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 32..38
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 43..47
FT /evidence="ECO:0007829|PDB:7JTK"
FT TURN 56..59
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 60..64
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 82..84
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 86..88
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 95..101
FT /evidence="ECO:0007829|PDB:7JTK"
FT TURN 106..108
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 114..116
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 119..124
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 132..137
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 139..143
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 144..146
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 148..157
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 163..166
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 171..173
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 181..194
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 201..203
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 222..224
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 225..230
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 241..249
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 270..277
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 282..287
FT /evidence="ECO:0007829|PDB:7JTK"
FT TURN 288..290
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 297..299
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 353..358
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 359..361
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 366..374
FT /evidence="ECO:0007829|PDB:7JTK"
FT TURN 378..384
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 387..394
FT /evidence="ECO:0007829|PDB:7JTK"
FT HELIX 406..414
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 420..422
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 428..436
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 452..460
FT /evidence="ECO:0007829|PDB:7JRJ"
FT HELIX 461..466
FT /evidence="ECO:0007829|PDB:7JRJ"
FT HELIX 473..476
FT /evidence="ECO:0007829|PDB:7JRJ"
FT HELIX 481..511
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 523..528
FT /evidence="ECO:0007829|PDB:7JRJ"
FT HELIX 539..542
FT /evidence="ECO:0007829|PDB:7JRJ"
FT HELIX 543..551
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 558..560
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 564..570
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 576..582
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 585..593
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 595..597
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 602..605
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 608..616
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 618..620
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 622..639
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 641..643
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 645..655
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 657..662
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 668..674
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 680..686
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 689..696
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 699..709
FT /evidence="ECO:0007829|PDB:7JRJ"
FT HELIX 711..713
FT /evidence="ECO:0007829|PDB:7JTK"
FT STRAND 714..716
FT /evidence="ECO:0007829|PDB:7JRJ"
FT HELIX 721..724
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 730..739
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 742..747
FT /evidence="ECO:0007829|PDB:7JTK"
FT TURN 768..770
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 775..777
FT /evidence="ECO:0007829|PDB:7JRJ"
FT TURN 793..795
FT /evidence="ECO:0007829|PDB:7JRJ"
FT STRAND 808..810
FT /evidence="ECO:0007829|PDB:7JRJ"
SQ SEQUENCE 814 AA; 87786 MW; D79D59405AB436ED CRC64;
MALGPFVLPF RGDQYSFGIN FKSSPEEKLN FDLSCVAFDV KGQLHDTLHA RKPTALDGAL
VKGFEKQALP EETVQVEGDD VIYMFPKKFE RQVEVLLFVA SAPSIPGKKH DLDSSSKLEF
AVSYSDVGGQ AFNQSFDLKP LAAQGGVSSI IVAVMYLQAE GGWTLRSVGD CHPFDSPGLI
VPELKQTILN LRDHHGVQLD AADAIQAIDP AERVPVTRQF QDQSLDEASA GRAAEPAPVK
KLRIDLSWTF WPPPPPTEEG EEPPEEPALE YNLVMYNKDG EEVQSISTGN REATGARAGR
PEPEEDEEEE KEEEKEEPEE GEEGEEGEGG EPKEPPPPPP APKVDPYEFK ERDVIYLDVP
DLPAEVRSMV LLVTNYDEEN GFTRVRTVRC RLVDVSNGEA PLPGSKAAVA AAAAAAEQGL
AAPPNPERVL ADYGVLSKYE DDKATTQVAL MKLYKEYADS AFNVFRGAGV DNVAAFIGQE
PDTIINQLKA YLEATKKQKA AEAAAAAAAE ESGEEITADP KPHVWRFRAL GLNFGGDSLE
AIEHDLKNLF AFDGDLAPGA ARDSDTSRSS FPNGDTYFGS YADDVKHGPG LYAFATGAGY
AGEYAGGKRH GRGVMVFPDG GTYVGEFVAD KFEGQGQYRY PDGSVYTGSW AAGQKHGPGV
YWDTARGCLR GEWKKGLLVG KGTYEQPALR FEGEFVRGMP AGTATYTLTG HRTLDMPCFA
AQHIQAEEGP TLALPCAYGI PPGSGDEPQL DEEGQPIEDT DKPPLPAHPK YEGLTFTAEQ
LPGAAPDTVF PPEEGKPVPI TAVPAFSVST GLVA
