RSP2_CAEEL
ID RSP2_CAEEL Reviewed; 281 AA.
AC Q23120;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Probable splicing factor, arginine/serine-rich 2;
DE AltName: Full=CeSRp40;
DE AltName: Full=RNA-binding protein srp-4;
GN Name=rsp-2; Synonyms=srp-4 {ECO:0000303|PubMed:10906451};
GN ORFNames=W02B12.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000312|EMBL:CAA91394.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:CAA91394.1};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=10747030; DOI=10.1093/emboj/19.7.1625;
RA Longman D., Johnstone I.L., Caceres J.F.;
RT "Functional characterization of SR and SR-related genes in Caenorhabditis
RT elegans.";
RL EMBO J. 19:1625-1637(2000).
RN [3] {ECO:0000305}
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10906451; DOI=10.1016/s0925-4773(00)00339-7;
RA Kawano T., Fujita M., Sakamoto H.;
RT "Unique and redundant functions of SR proteins, a conserved family of
RT splicing factors, in Caenorhabditis elegans development.";
RL Mech. Dev. 95:67-76(2000).
CC -!- FUNCTION: Plays a functionally redundant role in spermatogenesis and
CC growth rate control. Required for the development of somatic gonad
CC structures and for progression from larval stage to adulthood.
CC {ECO:0000269|PubMed:10747030, ECO:0000269|PubMed:10906451}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10906451}.
CC -!- PTM: Extensively phosphorylated on serine residues in the RS domain.
CC {ECO:0000250|UniProtKB:Q9NEW6}.
CC -!- MISCELLANEOUS: RNA-mediated interference (RNAi) of rsp-1 and rsp-2
CC result in reduced brood sizes and abnormal egg-laying behavior, and the
CC presence of unusual vacuolated structures in some of the secondary
CC spermatocytes, spermatids and spermatozoa. Rsp-2/rsp-6 RNAi caused
CC severe abnormalities in somatic gonad structures and in some cases
CC animals were arrested or dead at the larval stage.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000255}.
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DR EMBL; Z66521; CAA91394.1; -; Genomic_DNA.
DR PIR; T26084; T26084.
DR RefSeq; NP_496441.1; NM_064040.6.
DR AlphaFoldDB; Q23120; -.
DR SMR; Q23120; -.
DR BioGRID; 40054; 2.
DR DIP; DIP-26059N; -.
DR STRING; 6239.W02B12.2; -.
DR iPTMnet; Q23120; -.
DR EPD; Q23120; -.
DR PaxDb; Q23120; -.
DR PeptideAtlas; Q23120; -.
DR EnsemblMetazoa; W02B12.2.1; W02B12.2.1; WBGene00004699.
DR GeneID; 174747; -.
DR KEGG; cel:CELE_W02B12.2; -.
DR UCSC; W02B12.2; c. elegans.
DR CTD; 174747; -.
DR WormBase; W02B12.2; CE03762; WBGene00004699; rsp-2.
DR eggNOG; KOG0106; Eukaryota.
DR HOGENOM; CLU_012062_34_2_1; -.
DR InParanoid; Q23120; -.
DR OMA; CFSTHDD; -.
DR OrthoDB; 1315388at2759; -.
DR PhylomeDB; Q23120; -.
DR Reactome; R-CEL-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-CEL-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-CEL-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-CEL-72187; mRNA 3'-end processing.
DR Reactome; R-CEL-73856; RNA Polymerase II Transcription Termination.
DR PRO; PR:Q23120; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00004699; Expressed in embryo and 4 other tissues.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; ISS:WormBase.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0008406; P:gonad development; IGI:UniProtKB.
DR GO; GO:0007506; P:gonadal mesoderm development; IEA:UniProtKB-KW.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:WormBase.
DR GO; GO:0002119; P:nematode larval development; IGI:UniProtKB.
DR GO; GO:0040009; P:regulation of growth rate; IGI:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; NAS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IGI:UniProtKB.
DR CDD; cd12337; RRM1_SRSF4_like; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR035585; RRM1_SRSF4-like.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 3: Inferred from homology;
KW Developmental protein; Differentiation; Gonadal differentiation;
KW Growth regulation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; RNA-binding; Spermatogenesis.
FT CHAIN 1..281
FT /note="Probable splicing factor, arginine/serine-rich 2"
FT /id="PRO_0000081949"
FT DOMAIN 2..72
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 112..186
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 78..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 168..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 168..191
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..208
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..248
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 281 AA; 32421 MW; 06B467776DBDA061 CRC64;
MVRVYIGRLP NRASDRDVEH FFRGYGKLSD VIMKNGFGFV DFQDQRDADD AVHDLNGKEL
CGERVILEFP RRKVGYNEER SGSGFRGREP TFRKGGERQF SNRYSRPCST RFRLVIDNLS
TRYSWQDIKD HIRKLGIEPT YSEAHKRNVN QAIVCFTSHD DLRDAMNKLQ GEDLNGRKLK
CTDETRDRSR SRSPRRRSRS RSPTRSRSPP ARRRSPGSDR SDRKSRSASP KKRSDKRARS
ESKSRSRSGG RRSRSNSPPN RSPSPKKRRD NSSPRSGSAS P
