RSP4_CAEEL
ID RSP4_CAEEL Reviewed; 196 AA.
AC Q09511; Q95QN7;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Probable splicing factor, arginine/serine-rich 4;
DE AltName: Full=CeSC35;
DE AltName: Full=RNA-binding protein srp-2;
GN Name=rsp-4; Synonyms=srp-2; ORFNames=EEED8.7;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=10747030; DOI=10.1093/emboj/19.7.1625;
RA Longman D., Johnstone I.L., Caceres J.F.;
RT "Functional characterization of SR and SR-related genes in Caenorhabditis
RT elegans.";
RL EMBO J. 19:1625-1637(2000).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10906451; DOI=10.1016/s0925-4773(00)00339-7;
RA Kawano T., Fujita M., Sakamoto H.;
RT "Unique and redundant functions of SR proteins, a conserved family of
RT splicing factors, in Caenorhabditis elegans development.";
RL Mech. Dev. 95:67-76(2000).
CC -!- FUNCTION: May play a functionally redundant role in embryogenesis.
CC {ECO:0000269|PubMed:10747030, ECO:0000269|PubMed:10906451}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10906451}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q09511-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q09511-2; Sequence=VSP_012275, VSP_012276;
CC -!- PTM: Extensively phosphorylated on serine residues in the RS domain.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
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DR EMBL; FO081042; CCD68734.1; -; Genomic_DNA.
DR EMBL; FO081042; CCD68735.1; -; Genomic_DNA.
DR PIR; T15917; T15917.
DR RefSeq; NP_495013.1; NM_062612.4.
DR RefSeq; NP_495014.1; NM_062613.1. [Q09511-2]
DR AlphaFoldDB; Q09511; -.
DR SMR; Q09511; -.
DR BioGRID; 39259; 16.
DR DIP; DIP-26638N; -.
DR STRING; 6239.EEED8.7a; -.
DR iPTMnet; Q09511; -.
DR EPD; Q09511; -.
DR PaxDb; Q09511; -.
DR PeptideAtlas; Q09511; -.
DR PRIDE; Q09511; -.
DR EnsemblMetazoa; EEED8.7a.1; EEED8.7a.1; WBGene00004701. [Q09511-1]
DR EnsemblMetazoa; EEED8.7b.1; EEED8.7b.1; WBGene00004701. [Q09511-2]
DR EnsemblMetazoa; EEED8.7b.2; EEED8.7b.2; WBGene00004701. [Q09511-2]
DR GeneID; 173915; -.
DR UCSC; EEED8.7a; c. elegans. [Q09511-1]
DR CTD; 173915; -.
DR WormBase; EEED8.7a; CE01891; WBGene00004701; rsp-4. [Q09511-1]
DR WormBase; EEED8.7b; CE27910; WBGene00004701; rsp-4. [Q09511-2]
DR eggNOG; KOG4207; Eukaryota.
DR GeneTree; ENSGT00940000154883; -.
DR HOGENOM; CLU_012062_10_3_1; -.
DR InParanoid; Q09511; -.
DR OMA; PLIRCDV; -.
DR OrthoDB; 1524134at2759; -.
DR PRO; PR:Q09511; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00004701; Expressed in embryo and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:WormBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:WormBase.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IEA:InterPro.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR034893; SRSF2-like.
DR PANTHER; PTHR23147:SF145; PTHR23147:SF145; 1.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Developmental protein; mRNA processing;
KW mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding.
FT CHAIN 1..196
FT /note="Probable splicing factor, arginine/serine-rich 4"
FT /id="PRO_0000081951"
FT DOMAIN 19..97
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 91..196
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..110
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..143
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..183
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 116..126
FT /note="SRSPRRRSRSP -> TAAEPDVAELS (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_012275"
FT VAR_SEQ 127..196
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_012276"
SQ SEQUENCE 196 AA; 22588 MW; 61C047002F266DD5 CRC64;
MSRGGGGDRR AAPDINGLTS LKIDNLSYQT TPNDLRRTFE RYGDIGDVHI PRDKYSRQSK
GFGFVRFYER RDAEHALDRT DGKLVDGREL RVTLAKYDRP SDERGGRGGG GGRRRSRSPR
RRSRSPRYSR SRSPRRSRSR TRSPPSRDRR DSPDRRDNSR SRSRSPPPRE DGSPKERRSR
SRSASRSPSR SRSNSR