RSP5_CAEEL
ID RSP5_CAEEL Reviewed; 208 AA.
AC Q10021; Q95ZN0; Q95ZN1; Q95ZN2;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 30-JUL-2004, sequence version 3.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Probable splicing factor, arginine/serine-rich 5;
DE AltName: Full=CeSC35-2;
DE AltName: Full=RNA-binding protein srp-3;
GN Name=rsp-5; Synonyms=srp-3; ORFNames=T28D9.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=10747030; DOI=10.1093/emboj/19.7.1625;
RA Longman D., Johnstone I.L., Caceres J.F.;
RT "Functional characterization of SR and SR-related genes in Caenorhabditis
RT elegans.";
RL EMBO J. 19:1625-1637(2000).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10906451; DOI=10.1016/s0925-4773(00)00339-7;
RA Kawano T., Fujita M., Sakamoto H.;
RT "Unique and redundant functions of SR proteins, a conserved family of
RT splicing factors, in Caenorhabditis elegans development.";
RL Mech. Dev. 95:67-76(2000).
CC -!- FUNCTION: Plays a functionally redundant role in shifting germ cell
CC sexual differentiation in hermaphrodites. {ECO:0000269|PubMed:10747030,
CC ECO:0000269|PubMed:10906451}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10906451}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=a;
CC IsoId=Q10021-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q10021-2; Sequence=VSP_005908, VSP_005910;
CC Name=c;
CC IsoId=Q10021-3; Sequence=VSP_005906, VSP_005907;
CC Name=d;
CC IsoId=Q10021-4; Sequence=VSP_005909;
CC -!- PTM: Extensively phosphorylated on serine residues in the RS domain.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: RNA-mediated interference (RNAi) of rsp-5/rsp-6 resulted
CC in fertile animals that exhibit excessive sperm and delayed oocyte
CC production, and sterile animals that develop morphological
CC abnormalities of somatic gonads.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
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DR EMBL; FO081595; CCD72697.1; -; Genomic_DNA.
DR EMBL; FO081595; CCD72698.1; -; Genomic_DNA.
DR EMBL; FO081595; CCD72699.1; -; Genomic_DNA.
DR EMBL; FO081595; CCD72700.1; -; Genomic_DNA.
DR PIR; T16953; T16953.
DR RefSeq; NP_495307.3; NM_062906.6. [Q10021-1]
DR RefSeq; NP_495309.3; NM_062908.4.
DR AlphaFoldDB; Q10021; -.
DR SMR; Q10021; -.
DR IntAct; Q10021; 1.
DR STRING; 6239.T28D9.2a; -.
DR iPTMnet; Q10021; -.
DR EPD; Q10021; -.
DR PaxDb; Q10021; -.
DR PeptideAtlas; Q10021; -.
DR EnsemblMetazoa; T28D9.2a.1; T28D9.2a.1; WBGene00004702. [Q10021-1]
DR EnsemblMetazoa; T28D9.2b.1; T28D9.2b.1; WBGene00004702. [Q10021-2]
DR EnsemblMetazoa; T28D9.2b.2; T28D9.2b.2; WBGene00004702. [Q10021-2]
DR EnsemblMetazoa; T28D9.2b.3; T28D9.2b.3; WBGene00004702. [Q10021-2]
DR EnsemblMetazoa; T28D9.2b.4; T28D9.2b.4; WBGene00004702. [Q10021-2]
DR GeneID; 174073; -.
DR KEGG; cel:CELE_T28D9.2; -.
DR UCSC; T28D9.2d; c. elegans. [Q10021-1]
DR CTD; 174073; -.
DR WormBase; T28D9.2a; CE36572; WBGene00004702; rsp-5. [Q10021-1]
DR WormBase; T28D9.2b; CE36573; WBGene00004702; rsp-5. [Q10021-2]
DR eggNOG; KOG0106; Eukaryota.
DR HOGENOM; CLU_1344302_0_0_1; -.
DR InParanoid; Q10021; -.
DR OMA; MPRIYLG; -.
DR OrthoDB; 1315388at2759; -.
DR Reactome; R-CEL-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-CEL-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-CEL-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-CEL-72187; mRNA 3'-end processing.
DR Reactome; R-CEL-73856; RNA Polymerase II Transcription Termination.
DR PRO; PR:Q10021; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00004702; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; ISS:WormBase.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:WormBase.
DR GO; GO:0008380; P:RNA splicing; NAS:UniProtKB.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Differentiation; mRNA processing; mRNA splicing;
KW Nucleus; Phosphoprotein; Reference proteome; RNA-binding;
KW Sexual differentiation.
FT CHAIN 1..208
FT /note="Probable splicing factor, arginine/serine-rich 5"
FT /id="PRO_0000081952"
FT DOMAIN 2..74
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 71..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..123
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..193
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 194..208
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..128
FT /note="MPRLYLGKIPYNARERDVERFLKGYGKINNISMKYGFAFVDFEDSRDAEDAC
FT HDLDGKTMEGSSMRLVVEMARGKPRGNDRHGSRSPRRRSRSPRRRSRTPPRRRSRSRDR
FT KRSRRSRSRSSSRSRSP -> MLKTHAMIWMEKPWKEALCALSWKWLAENHAAMTVMDR
FT DHHDDVPALLDVVHVLHQEDVPALVIANVLADPVPEAPPDPDHQFVKVVAEANPVRRAR
FT KETLWVNYQYISIHFSFSPHSYQKPLFRLKE (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_005906"
FT VAR_SEQ 129..147
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_005907"
FT VAR_SEQ 147..166
FT /note="LKREASRSRSPLPAKDRSRT -> FHSSMRNQYHLQAHIAMAYT (in
FT isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_005908"
FT VAR_SEQ 148..208
FT /note="KREASRSRSPLPAKDRSRTRSGSPPKNGGDRKRSVSRGRSHSRDGSNRSVSR
FT SPSPGSPKD -> QLRANISVSYSSPLDSDSIKCKNYILGSVKRRDLVHHCQQKIEAEP
FT EVDLPQRTAEIESAA (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_005909"
FT VAR_SEQ 167..208
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_005910"
SQ SEQUENCE 208 AA; 23980 MW; 039E535C5FD86463 CRC64;
MPRLYLGKIP YNARERDVER FLKGYGKINN ISMKYGFAFV DFEDSRDAED ACHDLDGKTM
EGSSMRLVVE MARGKPRGND RHGSRSPRRR SRSPRRRSRT PPRRRSRSRD RKRSRRSRSR
SSSRSRSPVR ESRRRSESRS PSPKRDLKRE ASRSRSPLPA KDRSRTRSGS PPKNGGDRKR
SVSRGRSHSR DGSNRSVSRS PSPGSPKD
