RSPA_ECOLI
ID RSPA_ECOLI Reviewed; 404 AA.
AC P38104;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Starvation-sensing protein RspA;
GN Name=rspA {ECO:0000303|PubMed:7545940}; OrderedLocusNames=b1581, JW1573;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND OVEREXPRESSION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7545940; DOI=10.1126/science.7545940;
RA Huisman G.W., Kolter R.;
RT "Sensing starvation: a homoserine lactone-dependent signaling pathway in
RT Escherichia coli.";
RL Science 265:537-539(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TRANSCRIPTIONAL REGULATION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=22972332; DOI=10.1271/bbb.120273;
RA Sakihama Y., Mizoguchi H., Oshima T., Ogasawara N.;
RT "YdfH identified as a repressor of rspA by the use of reduced genome
RT Escherichia coli MGF-01.";
RL Biosci. Biotechnol. Biochem. 76:1688-1693(2012).
CC -!- FUNCTION: Probably involved in the degradation of homoserine lactone
CC (HSL) or of a metabolite of HSL that signals starvation.
CC -!- INDUCTION: Repressed by RspR. {ECO:0000269|PubMed:22972332}.
CC -!- MISCELLANEOUS: Overexpression prevents homoserine lactone-dependent
CC synthesis of the sigma S factor (rpoS). {ECO:0000269|PubMed:7545940}.
CC -!- SIMILARITY: Belongs to the mandelate racemase/muconate lactonizing
CC enzyme family. {ECO:0000305}.
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DR EMBL; L31628; AAA21685.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74653.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15285.1; -; Genomic_DNA.
DR PIR; I59589; I59589.
DR RefSeq; NP_416098.1; NC_000913.3.
DR RefSeq; WP_001295394.1; NZ_STEB01000003.1.
DR AlphaFoldDB; P38104; -.
DR SMR; P38104; -.
DR BioGRID; 4260233; 6.
DR DIP; DIP-10805N; -.
DR IntAct; P38104; 14.
DR STRING; 511145.b1581; -.
DR PaxDb; P38104; -.
DR PRIDE; P38104; -.
DR EnsemblBacteria; AAC74653; AAC74653; b1581.
DR EnsemblBacteria; BAA15285; BAA15285; BAA15285.
DR GeneID; 66674529; -.
DR GeneID; 946126; -.
DR KEGG; ecj:JW1573; -.
DR KEGG; eco:b1581; -.
DR PATRIC; fig|1411691.4.peg.681; -.
DR EchoBASE; EB2345; -.
DR eggNOG; COG4948; Bacteria.
DR HOGENOM; CLU_030273_6_1_6; -.
DR InParanoid; P38104; -.
DR OMA; DWDTRAY; -.
DR PhylomeDB; P38104; -.
DR BioCyc; EcoCyc:G6839-MON; -.
DR PRO; PR:P38104; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProt.
DR GO; GO:0009063; P:cellular amino acid catabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.120; -; 1.
DR Gene3D; 3.30.390.10; -; 1.
DR InterPro; IPR034589; D-mannonate_dehydratase-like.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR018110; Mandel_Rmase/mucon_lact_enz_CS.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR InterPro; IPR034593; Mandelate_racemase_DgoD-like.
DR InterPro; IPR013341; Mandelate_racemase_N_dom.
DR PANTHER; PTHR48080; PTHR48080; 1.
DR PANTHER; PTHR48080:SF6; PTHR48080:SF6; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR Pfam; PF02746; MR_MLE_N; 1.
DR SFLD; SFLDG00033; mannonate_dehydratase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; SSF51604; 1.
DR SUPFAM; SSF54826; SSF54826; 1.
DR PROSITE; PS00908; MR_MLE_1; 1.
DR PROSITE; PS00909; MR_MLE_2; 1.
PE 2: Evidence at transcript level;
KW Reference proteome.
FT CHAIN 1..404
FT /note="Starvation-sensing protein RspA"
FT /id="PRO_0000171260"
SQ SEQUENCE 404 AA; 45968 MW; A2C945E0716808AF CRC64;
MKIVKAEVFV TCPGRNFVTL KITTEDGITG LGDATLNGRE LSVASYLQDH LCPQLIGRDA
HRIEDIWQFF YKGAYWRRGP VTMSAISAVD MALWDIKAKA ANMPLYQLLG GASREGVMVY
CHTTGHSIDE ALDDYARHQE LGFKAIRVQC GIPGMKTTYG MSKGKGLAYE PATKGQWPEE
QLWSTEKYLD FMPKLFDAVR NKFGFNEHLL HDMHHRLTPI EAARFGKSIE DYRMFWMEDP
TPAENQECFR LIRQHTVTPI AVGEVFNSIW DCKQLIEEQL IDYIRTTLTH AGGITGMRRI
ADFASLYQVR TGSHGPSDLS PVCMAAALHF DLWVPNFGVQ EYMGYSEQML EVFPHNWTFD
NGYMHPGDKP GLGIEFDEKL AAKYPYEPAY LPVARLEDGT LWNW
