RSPH1_MOUSE
ID RSPH1_MOUSE Reviewed; 301 AA.
AC Q8VIG3; Q9DAL5;
DT 20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Radial spoke head 1 homolog;
DE AltName: Full=Male meiotic metaphase chromosome-associated acidic protein;
DE AltName: Full=Meichroacidin;
DE AltName: Full=Testis-specific gene A2 protein;
GN Name=Rsph1; Synonyms=Tsga2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Testis;
RX PubMed=9578619; DOI=10.1006/dbio.1998.8885;
RA Tsuchida J., Nishina Y., Wakabayashi N., Nozaki M., Sakai Y., Nishimune Y.;
RT "Molecular cloning and characterization of meichroacidin (male meiotic
RT metaphase chromosome-associated acidic protein).";
RL Dev. Biol. 197:67-76(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP INTERACTION WITH RSPH6A.
RX PubMed=30185526; DOI=10.1242/jcs.221648;
RA Abbasi F., Miyata H., Shimada K., Morohoshi A., Nozawa K., Matsumura T.,
RA Xu Z., Pratiwi P., Ikawa M.;
RT "RSPH6A is required for sperm flagellum formation and male fertility in
RT mice.";
RL J. Cell Sci. 131:0-0(2018).
RN [6]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=32203505; DOI=10.1371/journal.pgen.1008664;
RA Yoke H., Ueno H., Narita A., Sakai T., Horiuchi K., Shingyoji C.,
RA Hamada H., Shinohara K.;
RT "Rsph4a is essential for the triplet radial spoke head assembly of the
RT mouse motile cilia.";
RL PLoS Genet. 16:e1008664-e1008664(2020).
CC -!- FUNCTION: The specific expression during male germ cell development and
CC its characteristic localization suggest that it may play an important
CC role in male meiosis (PubMed:9578619). It is necessary for proper
CC building of the axonemal central pair and radial spokes (By
CC similarity). {ECO:0000250|UniProtKB:Q8WYR4,
CC ECO:0000269|PubMed:9578619}.
CC -!- SUBUNIT: Interacts with RSPH6A. {ECO:0000269|PubMed:30185526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:9578619}.
CC Chromosome {ECO:0000269|PubMed:9578619}. Cell projection, cilium
CC {ECO:0000269|PubMed:32203505}. Note=Cytoplasmic in late spermatocytes,
CC secondary spermatocytes and round spermatids. Gathered around metaphase
CC chromosomes during meiotic divisions. {ECO:0000269|PubMed:9578619}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VIG3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VIG3-2; Sequence=Not described;
CC -!- TISSUE SPECIFICITY: Expressed in the trachea, ependymal cells and
CC oviduct (at protein level) (PubMed:32203505). Germ cell specific.
CC Specifically expressed in testis, and to a lower extent in ovary. Not
CC expressed in somatic tissues (PubMed:9578619).
CC {ECO:0000269|PubMed:32203505, ECO:0000269|PubMed:9578619}.
CC -!- DEVELOPMENTAL STAGE: During male germ cell development it is not
CC detected until 12 days. Significant expression is detected from 14-day-
CC old through to adult testis. Expression is first detected in the
CC pachytene spermatocytes at stage V, becomes stronger from the late
CC pachytene spermatocytes to round spermatid stage, and then gradually
CC decreases as the morphogenesis proceeds further. Not expressed in germ
CC cells located in the first layer of the seminiferous epithelium
CC (spermatogonia, leptotene and zygotene spermatocytes).
CC {ECO:0000269|PubMed:9578619}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB83693.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB006535; BAB83693.1; ALT_FRAME; mRNA.
DR EMBL; AK005739; BAB24214.1; -; mRNA.
DR EMBL; BC049584; AAH49584.1; -; mRNA.
DR CCDS; CCDS28604.1; -. [Q8VIG3-1]
DR RefSeq; NP_079566.1; NM_025290.3. [Q8VIG3-1]
DR PDB; 7DMP; EM; 3.20 A; A/a=1-301.
DR PDBsum; 7DMP; -.
DR AlphaFoldDB; Q8VIG3; -.
DR SMR; Q8VIG3; -.
DR BioGRID; 204343; 1.
DR STRING; 10090.ENSMUSP00000024832; -.
DR iPTMnet; Q8VIG3; -.
DR PhosphoSitePlus; Q8VIG3; -.
DR REPRODUCTION-2DPAGE; Q8VIG3; -.
DR EPD; Q8VIG3; -.
DR MaxQB; Q8VIG3; -.
DR PaxDb; Q8VIG3; -.
DR PRIDE; Q8VIG3; -.
DR ProteomicsDB; 256938; -. [Q8VIG3-1]
DR Antibodypedia; 9520; 65 antibodies from 24 providers.
DR DNASU; 22092; -.
DR Ensembl; ENSMUST00000024832; ENSMUSP00000024832; ENSMUSG00000024033. [Q8VIG3-1]
DR GeneID; 22092; -.
DR KEGG; mmu:22092; -.
DR UCSC; uc008buu.1; mouse. [Q8VIG3-1]
DR CTD; 89765; -.
DR MGI; MGI:1194909; Rsph1.
DR VEuPathDB; HostDB:ENSMUSG00000024033; -.
DR eggNOG; KOG0231; Eukaryota.
DR GeneTree; ENSGT00940000157240; -.
DR HOGENOM; CLU_032017_2_0_1; -.
DR InParanoid; Q8VIG3; -.
DR OMA; EQHGEYK; -.
DR PhylomeDB; Q8VIG3; -.
DR TreeFam; TF329346; -.
DR BioGRID-ORCS; 22092; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Rsph1; mouse.
DR PRO; PR:Q8VIG3; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8VIG3; protein.
DR Bgee; ENSMUSG00000024033; Expressed in seminiferous tubule of testis and 149 other tissues.
DR ExpressionAtlas; Q8VIG3; baseline and differential.
DR Genevisible; Q8VIG3; MM.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0072687; C:meiotic spindle; IDA:MGI.
DR GO; GO:0031514; C:motile cilium; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001520; C:outer dense fiber; IDA:MGI.
DR GO; GO:0036126; C:sperm flagellum; IDA:MGI.
DR GO; GO:0035082; P:axoneme assembly; ISS:UniProtKB.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007286; P:spermatid development; IMP:MGI.
DR InterPro; IPR003409; MORN.
DR Pfam; PF02493; MORN; 7.
DR SMART; SM00698; MORN; 6.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell projection; Chromosome; Cytoplasm;
KW Meiosis; Reference proteome; Repeat.
FT CHAIN 1..301
FT /note="Radial spoke head 1 homolog"
FT /id="PRO_0000065663"
FT REPEAT 20..43
FT /note="MORN 1"
FT REPEAT 44..66
FT /note="MORN 2"
FT REPEAT 67..89
FT /note="MORN 3"
FT REPEAT 90..112
FT /note="MORN 4"
FT REPEAT 113..135
FT /note="MORN 5"
FT REPEAT 159..181
FT /note="MORN 6"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..301
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 258..279
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 280..301
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 39
FT /note="P -> S (in Ref. 1; BAB83693)"
FT /evidence="ECO:0000305"
FT STRAND 26..29
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 31..37
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 43..49
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 52..60
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 66..72
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 75..83
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 89..95
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 98..106
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 112..117
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 119..122
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 124..129
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 131..133
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 136..142
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 148..153
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 155..161
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 165..167
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 172..176
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 179..184
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 188..192
FT /evidence="ECO:0007829|PDB:7DMP"
FT STRAND 196..200
FT /evidence="ECO:0007829|PDB:7DMP"
SQ SEQUENCE 301 AA; 34181 MW; 704ABEFB94DFC90E CRC64;
MSDLGSEELE EEGENDLGEY EGERNEVGER HGHGKARLPN GDTYEGSYEF GKRHGQGTYK
FKNGARYTGD YVKNKKHGQG TFIYPDGSRY EGEWADDQRH GQGVYYYVNN DTYTGEWFNH
QRHGQGTYLY AETGSKYVGT WVHGQQEGAA ELIHLNHRYQ GKFMNKNPVG PGKYVFDIGC
EQHGEYRLTD TERGEEEEEE ETLVNIVPKW KALNITELAL WTPTLSEEQP PPEGQGQEEP
QGLTGVGDPS EDIQAEGFEG ELEPRGADED VDTFRQESQE NSYDIDQGNL NFDEEPSDLQ
D
