RSTA_ECOLI
ID RSTA_ECOLI Reviewed; 239 AA.
AC P52108;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Transcriptional regulatory protein RstA;
GN Name=rstA; Synonyms=urpT; OrderedLocusNames=b1608, JW1600;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Kuempel P.L.;
RL Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP MAPPING.
RX PubMed=1495392; DOI=10.1111/j.1365-2958.1992.tb00890.x;
RA Roecklein B.A., Kuempel P.L.;
RT "In vivo characterization of tus gene expression in Escherichia coli.";
RL Mol. Microbiol. 6:1655-1661(1992).
RN [6]
RP INDUCTION.
RC STRAIN=K12;
RX PubMed=12813061; DOI=10.1128/jb.185.13.3696-3702.2003;
RA Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T.,
RA Mori H., Ishihama A., Utsumi R.;
RT "Identification and molecular characterization of the Mg2+ stimulon of
RT Escherichia coli.";
RL J. Bacteriol. 185:3696-3702(2003).
RN [7]
RP PHOSPHORYLATION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=15522865; DOI=10.1074/jbc.m410104200;
RA Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.;
RT "Functional characterization in vitro of all two-component signal
RT transduction systems from Escherichia coli.";
RL J. Biol. Chem. 280:1448-1456(2005).
CC -!- FUNCTION: Member of the two-component regulatory system RstB/RstA.
CC -!- INTERACTION:
CC P52108; P21866: kdpE; NbExp=2; IntAct=EBI-558990, EBI-6403634;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Induced by low extracellular levels of magnesium via the
CC PhoQ/PhoP two-component regulatory system.
CC {ECO:0000269|PubMed:12813061}.
CC -!- PTM: Phosphorylated by RstB. {ECO:0000269|PubMed:15522865}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA82081.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA15346.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U41101; AAA82081.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC74680.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15346.1; ALT_INIT; Genomic_DNA.
DR PIR; B64917; B64917.
DR RefSeq; NP_416125.2; NC_000913.3.
DR RefSeq; WP_001092508.1; NZ_STEB01000003.1.
DR AlphaFoldDB; P52108; -.
DR SMR; P52108; -.
DR BioGRID; 4261763; 7.
DR DIP; DIP-10807N; -.
DR IntAct; P52108; 12.
DR STRING; 511145.b1608; -.
DR jPOST; P52108; -.
DR PaxDb; P52108; -.
DR PRIDE; P52108; -.
DR EnsemblBacteria; AAC74680; AAC74680; b1608.
DR EnsemblBacteria; BAA15346; BAA15346; BAA15346.
DR GeneID; 946199; -.
DR KEGG; ecj:JW1600; -.
DR KEGG; eco:b1608; -.
DR PATRIC; fig|1411691.4.peg.654; -.
DR EchoBASE; EB2982; -.
DR eggNOG; COG0745; Bacteria.
DR HOGENOM; CLU_000445_30_4_6; -.
DR InParanoid; P52108; -.
DR OMA; QIISEVW; -.
DR PhylomeDB; P52108; -.
DR BioCyc; EcoCyc:RSTA-MON; -.
DR PRO; PR:P52108; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..239
FT /note="Transcriptional regulatory protein RstA"
FT /id="PRO_0000081223"
FT DOMAIN 3..116
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 136..235
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 52
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 239 AA; 26704 MW; 409A1B3A2588A77D CRC64;
MNTIVFVEDD AEVGSLIAAY LAKHDMQVTV EPRGDQAEET ILRENPDLVL LDIMLPGKDG
MTICRDLRAK WSGPIVLLTS LDSDMNHILA LEMGACDYIL KTTPPAVLLA RLRLHLRQNE
QATLTKGLQE TSLTPYKALH FGTLTIDPIN RVVTLANTEI SLSTADFELL WELATHAGQI
MDRDALLKNL RGVSYDGLDR SVDVAISRLR KKLLDNAAEP YRIKTVRNKG YLFAPHAWE
