ID   RSUA_ECOL6              Reviewed;         231 AA.
AC   P0AA44; P33918;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE            EC=5.4.99.19;
DE   AltName: Full=16S pseudouridylate 516 synthase;
DE   AltName: Full=16S rRNA pseudouridine(516) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase A;
DE   AltName: Full=rRNA-uridine isomerase A;
GN   Name=rsuA; OrderedLocusNames=c2720;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-516 in
CC       16S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA;
CC         Xref=Rhea:RHEA:38867, Rhea:RHEA-COMP:10089, Rhea:RHEA-COMP:10090,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.19;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN81174.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE014075; AAN81174.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_001234850.1; NC_004431.1.
DR   AlphaFoldDB; P0AA44; -.
DR   SMR; P0AA44; -.
DR   STRING; 199310.c2720; -.
DR   EnsemblBacteria; AAN81174; AAN81174; c2720.
DR   GeneID; 66673920; -.
DR   KEGG; ecc:c2720; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_1_2_6; -.
DR   OMA; HVPKTYE; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; RNA-binding; rRNA processing.
FT   CHAIN           1..231
FT                   /note="Ribosomal small subunit pseudouridine synthase A"
FT                   /id="PRO_0000099967"
FT   DOMAIN          1..68
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        102
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   231 AA;  25865 MW;  04EB7154931C58F7 CRC64;
     MRLDKFIAQQ LGVSRAIAGR EIRGNRVTVD GEIVRNAAFK LLPEHDVAYD GNPLAQQHGP
     RYFMLNKPQG YVCSTDDPDH PTVLYFLDEP VAWKLHAAGR LDIDTTGLVL MTDDGQWSHR
     ITSPRHHCEK TYLVTLESPV ADDTAEQFAK GVQLHNEKDL TKPAVLEVIT PTQVRLTISE
     GRYHQVKRMF AAVGNHVVEL HRERIGGITL DADLAPGEYR PLTEEEIASV V