RSUA_HAEIN
ID RSUA_HAEIN Reviewed; 232 AA.
AC P45124;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE EC=5.4.99.19;
DE AltName: Full=16S pseudouridylate 516 synthase;
DE AltName: Full=16S rRNA pseudouridine(516) synthase;
DE AltName: Full=rRNA pseudouridylate synthase A;
DE AltName: Full=rRNA-uridine isomerase A;
GN Name=rsuA; OrderedLocusNames=HI_1243;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.59 ANGSTROMS).
RX PubMed=16511038; DOI=10.1107/s1744309105005920;
RA Matte A., Louie G.V., Sivaraman J., Cygler M., Burley S.K.;
RT "Structure of the pseudouridine synthase RsuA from Haemophilus
RT influenzae.";
RL Acta Crystallogr. F 61:350-354(2005).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-516 in
CC 16S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA;
CC Xref=Rhea:RHEA:38867, Rhea:RHEA-COMP:10089, Rhea:RHEA-COMP:10090,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.19;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; L42023; AAC22895.1; -; Genomic_DNA.
DR PIR; F64169; F64169.
DR RefSeq; NP_439399.1; NC_000907.1.
DR RefSeq; WP_005694303.1; NC_000907.1.
DR PDB; 1VIO; X-ray; 1.59 A; A/B=2-232.
DR PDBsum; 1VIO; -.
DR AlphaFoldDB; P45124; -.
DR SMR; P45124; -.
DR STRING; 71421.HI_1243; -.
DR DrugBank; DB01955; 1,4-Butanediol.
DR EnsemblBacteria; AAC22895; AAC22895; HI_1243.
DR KEGG; hin:HI_1243; -.
DR PATRIC; fig|71421.8.peg.1295; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_1_2_6; -.
DR OMA; HVPKTYE; -.
DR PhylomeDB; P45124; -.
DR BioCyc; HINF71421:G1GJ1-1273-MON; -.
DR EvolutionaryTrace; P45124; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..232
FT /note="Ribosomal small subunit pseudouridine synthase A"
FT /id="PRO_0000099969"
FT DOMAIN 1..68
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 102
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT HELIX 3..11
FT /evidence="ECO:0007829|PDB:1VIO"
FT HELIX 15..23
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 62..67
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:1VIO"
FT HELIX 83..86
FT /evidence="ECO:0007829|PDB:1VIO"
FT HELIX 91..94
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 96..99
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 106..113
FT /evidence="ECO:0007829|PDB:1VIO"
FT HELIX 115..122
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 130..138
FT /evidence="ECO:0007829|PDB:1VIO"
FT HELIX 144..150
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 165..168
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 170..179
FT /evidence="ECO:0007829|PDB:1VIO"
FT HELIX 185..192
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 197..205
FT /evidence="ECO:0007829|PDB:1VIO"
FT STRAND 219..221
FT /evidence="ECO:0007829|PDB:1VIO"
FT HELIX 224..229
FT /evidence="ECO:0007829|PDB:1VIO"
SQ SEQUENCE 232 AA; 26213 MW; E7590EE1A6BE8416 CRC64;
MRLDKFIAEN VGLTRSQATK AIRQSAVKIN GEIVKSGSVQ ISQEDEIYFE DELLTWIEEG
QYFMLNKPQG CVCSNDDGDY PTIYQFFDYP LAGKLHSAGR LDVDTTGLVL LTDDGQWSHR
ITSPKHHCEK TYLVTLADPV EENYSAACAE GILLRGEKEP TKPAKLEILD DYNVNLTISE
GRYHQVKRMF AALGNKVVGL HRWKIGDVVL DESLEEGEYR PLTQSEIEKL VK
