ID   RSUA_SHIFL              Reviewed;         231 AA.
AC   P0AA46; P33918;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE            EC=5.4.99.19;
DE   AltName: Full=16S pseudouridylate 516 synthase;
DE   AltName: Full=16S rRNA pseudouridine(516) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase A;
DE   AltName: Full=rRNA-uridine isomerase A;
GN   Name=rsuA; OrderedLocusNames=SF2270, S2399;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-516 in
CC       16S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA;
CC         Xref=Rhea:RHEA:38867, Rhea:RHEA-COMP:10089, Rhea:RHEA-COMP:10090,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.19;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; AE005674; AAN43789.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP17606.1; -; Genomic_DNA.
DR   RefSeq; NP_708082.1; NC_004337.2.
DR   RefSeq; WP_001234850.1; NZ_WPGW01000022.1.
DR   AlphaFoldDB; P0AA46; -.
DR   SMR; P0AA46; -.
DR   STRING; 198214.SF2270; -.
DR   PRIDE; P0AA46; -.
DR   EnsemblBacteria; AAN43789; AAN43789; SF2270.
DR   EnsemblBacteria; AAP17606; AAP17606; S2399.
DR   GeneID; 1024633; -.
DR   GeneID; 66673920; -.
DR   KEGG; sfl:SF2270; -.
DR   KEGG; sfx:S2399; -.
DR   PATRIC; fig|198214.7.peg.2719; -.
DR   HOGENOM; CLU_024979_1_2_6; -.
DR   OMA; HVPKTYE; -.
DR   OrthoDB; 1037615at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..231
FT                   /note="Ribosomal small subunit pseudouridine synthase A"
FT                   /id="PRO_0000099974"
FT   DOMAIN          1..68
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        102
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   231 AA;  25865 MW;  04EB7154931C58F7 CRC64;
     MRLDKFIAQQ LGVSRAIAGR EIRGNRVTVD GEIVRNAAFK LLPEHDVAYD GNPLAQQHGP
     RYFMLNKPQG YVCSTDDPDH PTVLYFLDEP VAWKLHAAGR LDIDTTGLVL MTDDGQWSHR
     ITSPRHHCEK TYLVTLESPV ADDTAEQFAK GVQLHNEKDL TKPAVLEVIT PTQVRLTISE
     GRYHQVKRMF AAVGNHVVEL HRERIGGITL DADLAPGEYR PLTEEEIASV V