ID   RSUA_VIBCH              Reviewed;         231 AA.
AC   Q9KRK5;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE            EC=5.4.99.19;
DE   AltName: Full=16S pseudouridylate 516 synthase;
DE   AltName: Full=16S rRNA pseudouridine(516) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase A;
DE   AltName: Full=rRNA-uridine isomerase A;
GN   Name=rsuA; OrderedLocusNames=VC_1635;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-516 in
CC       16S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA;
CC         Xref=Rhea:RHEA:38867, Rhea:RHEA-COMP:10089, Rhea:RHEA-COMP:10090,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.19;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; AE003852; AAF94786.1; -; Genomic_DNA.
DR   PIR; A82176; A82176.
DR   RefSeq; NP_231272.1; NC_002505.1.
DR   RefSeq; WP_001234872.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KRK5; -.
DR   SMR; Q9KRK5; -.
DR   STRING; 243277.VC_1635; -.
DR   DNASU; 2613890; -.
DR   EnsemblBacteria; AAF94786; AAF94786; VC_1635.
DR   GeneID; 57740291; -.
DR   KEGG; vch:VC_1635; -.
DR   PATRIC; fig|243277.26.peg.1563; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_1_2_6; -.
DR   OMA; HVPKTYE; -.
DR   BioCyc; VCHO:VC1635-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..231
FT                   /note="Ribosomal small subunit pseudouridine synthase A"
FT                   /id="PRO_0000099975"
FT   DOMAIN          1..67
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        101
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   231 AA;  26362 MW;  0B46DCE8E3AB497C CRC64;
     MRLDKFLCDA LGTTRKEATQ LLKSGEVTVD DVVQKSGAFK LKETSCVEWQ GREITLHGPR
     YIMMHKPDGV VCSHEDGFNQ TALTLLDEVN IQDLHFAGRL DVDTTGLLLI TDDGQWSHRV
     TSPKHKCEKT YRVWLADPIQ ADYAEQFNRG IELRGERELT LPAQLEVISE TEVLLTIHEG
     KYHQVKRMFA ALGNKVIGLH RERIGQITLD ETLEPGEYRY LTEEEVASIW K