RSUA_VIBPA
ID RSUA_VIBPA Reviewed; 233 AA.
AC Q87QD4;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE EC=5.4.99.19;
DE AltName: Full=16S pseudouridylate 516 synthase;
DE AltName: Full=16S rRNA pseudouridine(516) synthase;
DE AltName: Full=rRNA pseudouridylate synthase A;
DE AltName: Full=rRNA-uridine isomerase A;
GN Name=rsuA; OrderedLocusNames=VP1215;
OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=223926;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633;
RX PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-516 in
CC 16S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA;
CC Xref=Rhea:RHEA:38867, Rhea:RHEA-COMP:10089, Rhea:RHEA-COMP:10090,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.19;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; BA000031; BAC59478.1; -; Genomic_DNA.
DR RefSeq; NP_797594.1; NC_004603.1.
DR RefSeq; WP_005462415.1; NC_004603.1.
DR AlphaFoldDB; Q87QD4; -.
DR SMR; Q87QD4; -.
DR STRING; 223926.28806203; -.
DR EnsemblBacteria; BAC59478; BAC59478; BAC59478.
DR GeneID; 1188720; -.
DR KEGG; vpa:VP1215; -.
DR PATRIC; fig|223926.6.peg.1155; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_1_2_6; -.
DR OMA; HVPKTYE; -.
DR Proteomes; UP000002493; Chromosome 1.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..233
FT /note="Ribosomal small subunit pseudouridine synthase A"
FT /id="PRO_0000099976"
FT DOMAIN 1..67
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 101
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 233 AA; 26382 MW; 456A46A032426E6D CRC64;
MRLDKYLCDA LGATRKQATK IIKSGEVLVD GEVQKSGSFK VPEGGVVEWD GREVGAPGPR
YIMLYKPADF VCSHEDGYNP TAFVLLDEPK VENLHFAGRL DVDTTGLVLI TDDGQWSHRI
TSPKHKCKKT YRVWLADAIQ PDYVEKFAQG IELRNEREAT LPAHLEIVNE AENEVLLTIV
EGKYHQVKRM FAALGNKVEL LHRERIGDIV LDDTLEPGDY RYLTQEEVDS VWN
