ID   RSUA_VIBVU              Reviewed;         233 AA.
AC   Q8D8X2;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE            EC=5.4.99.19;
DE   AltName: Full=16S pseudouridylate 516 synthase;
DE   AltName: Full=16S rRNA pseudouridine(516) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase A;
DE   AltName: Full=rRNA-uridine isomerase A;
GN   Name=rsuA; OrderedLocusNames=VV1_2843;
OS   Vibrio vulnificus (strain CMCP6).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=216895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-516 in
CC       16S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA;
CC         Xref=Rhea:RHEA:38867, Rhea:RHEA-COMP:10089, Rhea:RHEA-COMP:10090,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.19;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; AE016795; AAO11180.1; -; Genomic_DNA.
DR   RefSeq; WP_011080669.1; NC_004459.3.
DR   AlphaFoldDB; Q8D8X2; -.
DR   SMR; Q8D8X2; -.
DR   EnsemblBacteria; AAO11180; AAO11180; VV1_2843.
DR   KEGG; vvu:VV1_2843; -.
DR   HOGENOM; CLU_024979_1_2_6; -.
DR   OMA; HVPKTYE; -.
DR   Proteomes; UP000002275; Chromosome 1.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; RNA-binding; rRNA processing.
FT   CHAIN           1..233
FT                   /note="Ribosomal small subunit pseudouridine synthase A"
FT                   /id="PRO_0000099977"
FT   DOMAIN          1..67
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        101
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   233 AA;  26592 MW;  9B1A9249A80C8912 CRC64;
     MRLDKFLCDA LGATRKEATK ILKSGEVTVN EQVQKSGAVK VTEECVVEWQ GRELAMQGPR
     YIMLYKPEGF VCSHEDGFNH TAFVLLDEVK MDELHFAGRL DVDTTGLVLI TDDGKWSHRI
     TSPKHKCDKT YRVWLADPIG SDYAEKLAEG VQLRNEKELT LPAQMTIVNA DENEVLLTIH
     EGKYHQVKRM FAALGNKVEQ LHRERIGAIE LDESLEPGEY RYLTQEEIDS VWK