RSUA_YERPE
ID RSUA_YERPE Reviewed; 235 AA.
AC Q8ZGM2; Q0WHE3;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE EC=5.4.99.19;
DE AltName: Full=16S pseudouridylate 516 synthase;
DE AltName: Full=16S rRNA pseudouridine(516) synthase;
DE AltName: Full=rRNA pseudouridylate synthase A;
DE AltName: Full=rRNA-uridine isomerase A;
GN Name=rsuA; OrderedLocusNames=YPO1266, y2917, YP_1322;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-516 in
CC 16S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA;
CC Xref=Rhea:RHEA:38867, Rhea:RHEA-COMP:10089, Rhea:RHEA-COMP:10090,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.19;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; AL590842; CAL19923.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM86468.1; -; Genomic_DNA.
DR EMBL; AE017042; AAS61565.1; -; Genomic_DNA.
DR PIR; AI0154; AI0154.
DR RefSeq; WP_002208832.1; NZ_WUCM01000013.1.
DR RefSeq; YP_002346295.1; NC_003143.1.
DR AlphaFoldDB; Q8ZGM2; -.
DR SMR; Q8ZGM2; -.
DR STRING; 214092.YPO1266; -.
DR PaxDb; Q8ZGM2; -.
DR DNASU; 1147864; -.
DR EnsemblBacteria; AAM86468; AAM86468; y2917.
DR EnsemblBacteria; AAS61565; AAS61565; YP_1322.
DR GeneID; 57977403; -.
DR KEGG; ype:YPO1266; -.
DR KEGG; ypk:y2917; -.
DR KEGG; ypm:YP_1322; -.
DR PATRIC; fig|214092.21.peg.1571; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_1_2_6; -.
DR OMA; HVPKTYE; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..235
FT /note="Ribosomal small subunit pseudouridine synthase A"
FT /id="PRO_0000099978"
FT DOMAIN 1..68
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 102
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 235 AA; 26541 MW; 474F1E15374F2DED CRC64;
MRLDKFLSQQ LGVSRALVAR ELRAKRVTID GEVVKSGAIK LTPEQDVRFD GNPLQQMVGP
RYFMLNKPQG YVCSTEDPDH PTVLYFLDEP VAYKLHAAGR LDIDTTGLVL MTDDGQWSHR
VTSPRHHCEK TYLVTLEHPL SDDTAQQFAD GVQLYNEKSL TKPAQLDVIE PQRVRLTISE
GRYHQVKRMF AAVGNRVIEL HRERIGDITL DDDLAPGEYR PLTTEEIASV GAPHL
