RSXA_ECOLI
ID RSXA_ECOLI Reviewed; 193 AA.
AC P0A766; P76181; Q2MB70;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Ion-translocating oxidoreductase complex subunit A {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
DE EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
DE AltName: Full=Rsx electron transport complex subunit A {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000305};
GN Name=rsxA {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000303|PubMed:12773378};
GN Synonyms=rnfA, ydgL; OrderedLocusNames=b1627, JW1619;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP TOPOLOGY.
RC STRAIN=K12 / JM109 / ATCC 53323;
RX PubMed=10411911; DOI=10.1073/pnas.96.15.8540;
RA Saeaef A., Johansson M., Wallin E., von Heijne G.;
RT "Divergent evolution of membrane protein topology: the Escherichia coli
RT RnfA and RnfE homologues.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:8540-8544(1999).
RN [4]
RP FUNCTION, SUBUNIT, AND GENE NAME.
RX PubMed=12773378; DOI=10.1093/emboj/cdg252;
RA Koo M.S., Lee J.H., Rah S.Y., Yeo W.S., Lee J.W., Lee K.L., Koh Y.S.,
RA Kang S.O., Roe J.H.;
RT "A reducing system of the superoxide sensor SoxR in Escherichia coli.";
RL EMBO J. 22:2614-2622(2003).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC transfer with translocation of ions across the membrane (By
CC similarity). Required to maintain the reduced state of SoxR. Probably
CC transfers electron from NAD(P)H to SoxR (PubMed:12773378).
CC {ECO:0000255|HAMAP-Rule:MF_00459, ECO:0000269|PubMed:12773378}.
CC -!- SUBUNIT: The complex is composed of six subunits: RsxA, RsxB, RsxC,
CC RsxD, RsxE and RsxG. {ECO:0000255|HAMAP-Rule:MF_00459,
CC ECO:0000305|PubMed:12773378}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00459, ECO:0000269|PubMed:15919996}; Multi-pass membrane
CC protein {ECO:0000255|HAMAP-Rule:MF_00459}.
CC -!- SIMILARITY: Belongs to the NqrDE/RnfAE family. {ECO:0000255|HAMAP-
CC Rule:MF_00459}.
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DR EMBL; U00096; AAC74699.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76486.1; -; Genomic_DNA.
DR PIR; E64919; E64919.
DR RefSeq; NP_416144.1; NC_000913.3.
DR RefSeq; WP_000133193.1; NZ_STEB01000003.1.
DR AlphaFoldDB; P0A766; -.
DR SMR; P0A766; -.
DR BioGRID; 4261327; 41.
DR STRING; 511145.b1627; -.
DR TCDB; 3.D.6.1.4; the ion (h(+) or na(+))-translocating nadh:ferredoxin oxidoreductase (nfo or rnf) family.
DR PaxDb; P0A766; -.
DR PRIDE; P0A766; -.
DR EnsemblBacteria; AAC74699; AAC74699; b1627.
DR EnsemblBacteria; BAE76486; BAE76486; BAE76486.
DR GeneID; 66674481; -.
DR GeneID; 946148; -.
DR KEGG; ecj:JW1619; -.
DR KEGG; eco:b1627; -.
DR PATRIC; fig|1411691.4.peg.634; -.
DR EchoBASE; EB3692; -.
DR eggNOG; COG4657; Bacteria.
DR HOGENOM; CLU_095255_1_0_6; -.
DR InParanoid; P0A766; -.
DR OMA; CPFFGVS; -.
DR PhylomeDB; P0A766; -.
DR BioCyc; EcoCyc:G6871-MON; -.
DR PRO; PR:P0A766; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00459; RsxA_RnfA; 1.
DR InterPro; IPR011293; Ion_transpt_RnfA/RsxA.
DR InterPro; IPR003667; NqrDE/RnfAE.
DR Pfam; PF02508; Rnf-Nqr; 1.
DR PIRSF; PIRSF006102; NQR_DE; 1.
DR TIGRFAMs; TIGR01943; rnfA; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Electron transport; Membrane;
KW Reference proteome; Translocase; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..193
FT /note="Ion-translocating oxidoreductase complex subunit A"
FT /id="PRO_0000214289"
FT TOPO_DOM 1..4
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TOPO_DOM 26..38
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:10411911"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TOPO_DOM 60..62
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:10411911"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TOPO_DOM 84..101
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:10411911"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TOPO_DOM 123..133
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:10411911"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TOPO_DOM 155..170
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:10411911"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00459"
FT TOPO_DOM 192..193
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:10411911,
FT ECO:0000269|PubMed:15919996"
SQ SEQUENCE 193 AA; 20898 MW; 525955B3760398D7 CRC64;
MTDYLLLFVG TVLVNNFVLV KFLGLCPFMG VSKKLETAMG MGLATTFVMT LASICAWLID
TWILIPLNLI YLRTLAFILV IAVVVQFTEM VVRKTSPVLY RLLGIFLPLI TTNCAVLGVA
LLNINLGHNF LQSALYGFSA AVGFSLVMVL FAAIRERLAV ADVPAPFRGN AIALITAGLM
SLAFMGFSGL VKL
