BCL9_DROME
ID BCL9_DROME Reviewed; 1469 AA.
AC Q961D9; Q9V4D2;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Protein BCL9 homolog;
DE AltName: Full=Protein legless;
GN Name=lgs; Synonyms=BCL9; ORFNames=CG2041;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 6-1469, AND MUTAGENESIS OF GLY-514; LEU-534
RP AND ILE-537.
RX PubMed=11955446; DOI=10.1016/s0092-8674(02)00679-7;
RA Kramps T., Peter O., Brunner E., Nellen D., Froesch B., Chatterjee S.,
RA Murone M., Zuellig S., Basler K.;
RT "Wnt/wingless signaling requires BCL9/legless-mediated recruitment of
RT pygopus to the nuclear beta-catenin-TCF complex.";
RL Cell 109:47-60(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; THR-11; SER-206; THR-208;
RP SER-210; SER-883; SER-905 AND SER-911, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Involved in signal transduction through the Wnt pathway.
CC -!- SUBUNIT: Binds to ARM and PYGO.
CC -!- INTERACTION:
CC Q961D9; P18824: arm; NbExp=5; IntAct=EBI-85519, EBI-216128;
CC Q961D9; Q9V9W8: pygo; NbExp=4; IntAct=EBI-85519, EBI-152653;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically
CC throughout development.
CC -!- SIMILARITY: Belongs to the BCL9 family. {ECO:0000305}.
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DR EMBL; AE014135; AAF59345.2; -; Genomic_DNA.
DR EMBL; AY051651; AAK93075.1; -; mRNA.
DR EMBL; AF457205; AAL91368.1; -; mRNA.
DR RefSeq; NP_651922.1; NM_143665.4.
DR PDB; 3ZPV; X-ray; 2.68 A; 0/2/4/6/8/B/D/F/H/J/L/N/P/R/T/V/X/Z=321-353.
DR PDBsum; 3ZPV; -.
DR AlphaFoldDB; Q961D9; -.
DR SMR; Q961D9; -.
DR BioGRID; 68624; 10.
DR DIP; DIP-33392N; -.
DR IntAct; Q961D9; 8.
DR MINT; Q961D9; -.
DR STRING; 7227.FBpp0088180; -.
DR iPTMnet; Q961D9; -.
DR PaxDb; Q961D9; -.
DR EnsemblMetazoa; FBtr0089111; FBpp0088180; FBgn0039907.
DR GeneID; 43791; -.
DR KEGG; dme:Dmel_CG2041; -.
DR UCSC; CG2041-RA; d. melanogaster.
DR CTD; 43791; -.
DR FlyBase; FBgn0039907; lgs.
DR VEuPathDB; VectorBase:FBgn0039907; -.
DR eggNOG; ENOG502QYJW; Eukaryota.
DR HOGENOM; CLU_251426_0_0_1; -.
DR InParanoid; Q961D9; -.
DR OMA; SANKNCF; -.
DR OrthoDB; 92706at2759; -.
DR PhylomeDB; Q961D9; -.
DR Reactome; R-DME-201722; Formation of the beta-catenin:TCF transactivating complex.
DR Reactome; R-DME-209407; Transport of ARM to the nucleus.
DR Reactome; R-DME-209421; Transcription activation by ARM.
DR SignaLink; Q961D9; -.
DR BioGRID-ORCS; 43791; 0 hits in 1 CRISPR screen.
DR ChiTaRS; lgs; fly.
DR GenomeRNAi; 43791; -.
DR PRO; PR:Q961D9; -.
DR Proteomes; UP000000803; Chromosome 4.
DR Bgee; FBgn0039907; Expressed in cleaving embryo and 26 other tissues.
DR ExpressionAtlas; Q961D9; baseline and differential.
DR Genevisible; Q961D9; DM.
DR GO; GO:1990907; C:beta-catenin-TCF complex; IDA:FlyBase.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; IGI:FlyBase.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IMP:FlyBase.
DR GO; GO:0035293; P:chitin-based larval cuticle pattern formation; IMP:FlyBase.
DR GO; GO:0008587; P:imaginal disc-derived wing margin morphogenesis; IMP:FlyBase.
DR GO; GO:0035223; P:leg disc pattern formation; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IPI:UniProtKB.
DR GO; GO:0007367; P:segment polarity determination; IMP:UniProtKB.
DR GO; GO:0007491; P:sternite morphogenesis; IMP:FlyBase.
DR InterPro; IPR024670; BCL9_beta-catenin-bd_dom.
DR Pfam; PF11502; BCL9; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; Nucleus; Phosphoprotein;
KW Reference proteome; Segmentation polarity protein; Wnt signaling pathway.
FT CHAIN 1..1469
FT /note="Protein BCL9 homolog"
FT /id="PRO_0000064884"
FT REGION 1..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 161..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 422..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 454..474
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 511..555
FT /note="ARM-binding"
FT REGION 728..830
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 844..913
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 961..991
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 71..131
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 172..186
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 735..758
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 773..830
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 9
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 11
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 206
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 208
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 210
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 883
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 905
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 911
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MUTAGEN 514
FT /note="G->E: In allele lgs-21L."
FT /evidence="ECO:0000269|PubMed:11955446"
FT MUTAGEN 534
FT /note="L->F: In allele lgs-17E; segment polarity
FT phenotype."
FT /evidence="ECO:0000269|PubMed:11955446"
FT MUTAGEN 537
FT /note="I->K: In allele lgs-17P."
FT /evidence="ECO:0000269|PubMed:11955446"
FT STRAND 324..327
FT /evidence="ECO:0007829|PDB:3ZPV"
FT HELIX 328..339
FT /evidence="ECO:0007829|PDB:3ZPV"
FT HELIX 346..351
FT /evidence="ECO:0007829|PDB:3ZPV"
SQ SEQUENCE 1469 AA; 153759 MW; 5672E01B7200ED08 CRC64;
MLSTTMPRSP TQQQPQPNSD ASSTSASGSN PGAAIGNGDS AASRSSPKTL NSEPFSTLSP
DQIKLTPEEG TEKSGLSTSD KAATGGAPGS GNNLPEGQTM LRQNSTSTIN SCLVASPQNS
SEHSNSSNVS ATVGLTQMVD CDEQSKKNKC SVKDEEAEIS SNKAKGQAAG GGCETGSTSS
LTVKEEPTDV LGSLVNMKKE ERENHSPTMS PVGFGSIGNA QDNSATPVKI ERISNDSTTE
KKGSSLTMNN DEMSMEGCNQ LNPDFINESL NNPAISSILV SGVGPIPGIG VGAGTGNLLT
ANANGISSGS SNCLDYMQQQ NHIFVFSTQL ANKGAESVLS GQFQTIIAYH CTQPATKSFL
EDFFMKNPLK INKLQRHNSV GMPWIGMGQV GLTPPNPVAK ITQQQPHTKT VGLLKPQFNQ
HENSKRSTVS APSNSFVDQS DPMGNETELM CWEGGSSNTS RSGQNSRNHV DSISTSSESQ
AIKILEAAGV DLGQVTKGSD PGLTTENNIV SLQGVKVPDE NLTPQQRQHR EEQLAKIKKM
NQFLFPENEN SVGANVSSQI TKIPGDLMMG MSGGGGGSII NPTMRQLHMP GNAKSELLSA
TSSGLSEDVM HPGDVISDMG AVIGCNNNQK TSVQCGSGVG VVTGTTAAGV NVNMHCSSSG
APNGNMMGSS TDMLASFGNT SCNVIGTAPD MSKEVLNQDS RTHSHQGGVA QMEWSKIQHQ
FFEERLKGGK PRQVTGTVVP QQQTPSGSGG NSLNNQVRPL QGPPPPYHSI QRSASVPIAT
QSPNPSSPNN LSLPSPRTTA AVMGLPTNSP SMDGTGSLSG SVPQANTSTV QAGTTTVLSA
NKNCFQADTP SPSNQNRSRN TGSSSVLTHN LSSNPSTPLS HLSPKEFESF GQSSAGDNMK
SRRPSPQGQR SPVNSLIEAN KDVRFAASSP GFNPHPHMQS NSNSALNAYK MGSTNIQMER
QASAQGGSVQ FSRRSDNIPL NPNSGNRPPP NKMTQNFDPI SSLAQMSQQL TSCVSSMGSP
AGTGGMTMMG GPGPSDINIE HGIISGLDGS GIDTINQNNC HSMNVVMNSM GPRMLNPKMC
VAGGPNGPPG FNPNSPNGGL RENSIGSGCG SANSSNFQGV VPPGARMMGR MPVNFGSNFN
PNIQVKASTP NTIQYMPVRA QNANNNNNNG ANNVRMPPSL EFLQRYANPQ MGAVGNGSPI
CPPSASDGTP GMPGLMAGPG AGGMLMNSSG EQHQNKITNN PGASNGINFF QNCNQMSIVD
EEGGLPGHDG SMNIGQPSMI RGMRPHAMRP NVMGARMPPV NRQIQFAQSS DGIDCVGDPS
SFFTNASCNS AGPHMFGSAQ QANQPKTQHI KNIPSGMCQN QSGLAVAQGQ IQLHGQGHAQ
GQSLIGPTNN NLMSTAGSVS ATNGVSGINF VGPSSTDLKY AQQYHSFQQQ LYATNTRSQQ
QQHMHQQHQS NMITMPPNLS PNPTFFVNK
