BCLA3_MOUSE
ID BCLA3_MOUSE Reviewed; 752 AA.
AC A2AG58; A2AG56; A2AG57; Q3UM32; Q8BRL2;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=BCLAF1 and THRAP3 family member 3 {ECO:0000250|UniProtKB:A2AJT9};
GN Name=Bclaf3 {ECO:0000250|UniProtKB:A2AJT9};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain cortex, and Mammary gland;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-205 AND SER-592, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2AG58-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2AG58-2; Sequence=VSP_025460;
CC -!- SIMILARITY: Belongs to the BCLAF1/THRAP3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAM23340.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AK044001; BAC31733.1; -; mRNA.
DR EMBL; AK145159; BAE26266.1; -; mRNA.
DR EMBL; AL672284; CAM23340.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL672284; CAM23341.1; -; Genomic_DNA.
DR EMBL; AL672284; CAM23342.1; -; Genomic_DNA.
DR CCDS; CCDS30503.2; -. [A2AG58-1]
DR CCDS; CCDS90769.1; -. [A2AG58-2]
DR RefSeq; NP_001028644.2; NM_001033472.2. [A2AG58-1]
DR RefSeq; XP_017174023.1; XM_017318534.1. [A2AG58-2]
DR RefSeq; XP_017174024.1; XM_017318535.1.
DR AlphaFoldDB; A2AG58; -.
DR STRING; 10090.ENSMUSP00000108083; -.
DR iPTMnet; A2AG58; -.
DR PhosphoSitePlus; A2AG58; -.
DR EPD; A2AG58; -.
DR jPOST; A2AG58; -.
DR MaxQB; A2AG58; -.
DR PaxDb; A2AG58; -.
DR PeptideAtlas; A2AG58; -.
DR PRIDE; A2AG58; -.
DR ProteomicsDB; 273662; -. [A2AG58-1]
DR ProteomicsDB; 273663; -. [A2AG58-2]
DR Antibodypedia; 9758; 34 antibodies from 11 providers.
DR DNASU; 382252; -.
DR Ensembl; ENSMUST00000057180; ENSMUSP00000051031; ENSMUSG00000044150. [A2AG58-2]
DR Ensembl; ENSMUST00000112464; ENSMUSP00000108083; ENSMUSG00000044150. [A2AG58-1]
DR GeneID; 382252; -.
DR KEGG; mmu:382252; -.
DR UCSC; uc009usr.1; mouse. [A2AG58-1]
DR UCSC; uc012hrd.1; mouse. [A2AG58-2]
DR CTD; 256643; -.
DR MGI; MGI:2685992; Bclaf3.
DR VEuPathDB; HostDB:ENSMUSG00000044150; -.
DR eggNOG; ENOG502S4TN; Eukaryota.
DR GeneTree; ENSGT00950000183163; -.
DR HOGENOM; CLU_024256_0_0_1; -.
DR InParanoid; A2AG58; -.
DR OMA; KWHEDEF; -.
DR OrthoDB; 442192at2759; -.
DR PhylomeDB; A2AG58; -.
DR TreeFam; TF335939; -.
DR BioGRID-ORCS; 382252; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Bclaf3; mouse.
DR PRO; PR:A2AG58; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; A2AG58; protein.
DR Bgee; ENSMUSG00000044150; Expressed in placenta labyrinth and 216 other tissues.
DR ExpressionAtlas; A2AG58; baseline and differential.
DR Genevisible; A2AG58; MM.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR029199; THRAP3_BCLAF1.
DR PANTHER; PTHR15268; PTHR15268; 1.
DR Pfam; PF15440; THRAP3_BCLAF1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Isopeptide bond; Mitochondrion; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..752
FT /note="BCLAF1 and THRAP3 family member 3"
FT /id="PRO_0000287435"
FT REGION 1..114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..61
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..111
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 214..252
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 15
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:A2AJT9"
FT MOD_RES 17
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:A2AJT9"
FT MOD_RES 78
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:A2AJT9"
FT MOD_RES 80
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:A2AJT9"
FT MOD_RES 205
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 592
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CROSSLNK 416
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:A2AJT9"
FT VAR_SEQ 597..752
FT /note="NHQSPSFSKVKTIRADGFQKPPHFIKSNFRKFIQKPYINYTMQRKDAIDQKI
FT FRVEENHQNRRGSKGSFKNFLGGRFQPHYKSHLVQKSMYIQAKYQRLRFAGPRGFITNK
FT FRNRFLRKKKEYPVLPRTNNLELLQVEPTLYEDLTEHLIFVRNWN -> TILCREKMPL
FT IRRYLELRKIIRTEEALKDLLRTFWVADSSLIINHTWYRRACIFRLNTSAYGLLDQEDL
FT LPINSETDF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025460"
FT CONFLICT 166
FT /note="K -> Q (in Ref. 1; BAE26266)"
FT /evidence="ECO:0000305"
FT CONFLICT 392
FT /note="S -> N (in Ref. 1; BAE26266)"
FT /evidence="ECO:0000305"
FT CONFLICT 591
FT /note="A -> T (in Ref. 1; BAE26266)"
FT /evidence="ECO:0000305"
FT CONFLICT A2AG58-2:657
FT /note="Y -> C (in Ref. 1; BAE26266)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 752 AA; 90111 MW; 85DFCD631B080798 CRC64;
MARSRSRSPR WKQRSLSPQS RNFEYHEERH FHGHYDPEYR HDQQRPFTWR MDDEKHGQNK
PRIPPRVNSY HRSYVNRSPS PNVKPVEKFD TYKPHQEYFP GRGDDDRRSQ YMPTYTESAA
TYMEHERDCY IPTVQGRYTP DDHRGRGRGS GRGEKPPQMS LGKPPKMSLG KPPQMSLADS
LRFKEKWHED ELRHQRVQEE SYPQSPRRGS EDFGTRNPFQ KRYPEDHDFR KYGYTSKRPT
DAARYENRDP ARIPKWKPEH SFLPFQEKKE EWSFGAQGHR YTEREYPERS STTRVSYDYR
HKHHKLSESE QDFPDGRFHK HLKEEDRKYS SIKAPANREL DCFSTTRGRE IENEQINGPF
YLYNKNSVSY NHTNIKDADL EPCNDKWKKK ISKEDCRKEN ASFSKQFDTS PKPEEKCYSL
IKKKPLSVKV DRNKTDTFRS TSRYSAERQI SHDLVAIGKT SDNFHPVFQH LDSTQNPENK
PTEEFAQEII TLIHKVKADS FVTPDITLNE RFSRIKNRQD ADFNQTKSNS DPEFHRRIDM
SLDDFQNKYT MVYEPDKTLV KVIEPNDLRH DIERRRKERL QNEDENIFHM ASPTERNHQS
PSFSKVKTIR ADGFQKPPHF IKSNFRKFIQ KPYINYTMQR KDAIDQKIFR VEENHQNRRG
SKGSFKNFLG GRFQPHYKSH LVQKSMYIQA KYQRLRFAGP RGFITNKFRN RFLRKKKEYP
VLPRTNNLEL LQVEPTLYED LTEHLIFVRN WN
