RT12_DROME
ID RT12_DROME Reviewed; 140 AA.
AC P10735; Q5U1D4; Q9NEH6; Q9U1J6; Q9V3R2;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=40S ribosomal protein S12, mitochondrial;
DE Short=MRP-S12;
DE Short=S12mt;
DE AltName: Full=MT-RPS12;
DE AltName: Full=Protein technical knockout locus;
DE Flags: Precursor;
GN Name=tko; ORFNames=CG7925;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3117373; DOI=10.1016/0092-8674(87)90144-9;
RA Royden C.S., Pirrotta V., Jan L.Y.;
RT "The tko locus, site of a behavioral mutation in D. melanogaster, codes for
RT a protein homologous to prokaryotic ribosomal protein S12.";
RL Cell 51:165-173(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP LEU-85.
RX PubMed=16120347; DOI=10.1016/s1567-7249(03)00077-1;
RA Toivonen J.M., Manjiry S., Touraille S., Alziari S., O'Dell K.M.C.,
RA Jacobs H.T.;
RT "Gene dosage and selective expression modify phenotype in a Drosophila
RT model of human mitochondrial disease.";
RL Mitochondrion 3:83-96(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Mitochondrion.
CC -!- DEVELOPMENTAL STAGE: Abundantly expressed through all stages of the
CC life cycle. {ECO:0000269|PubMed:16120347}.
CC -!- MISCELLANEOUS: Mutation of tko causes a behavioral mutation ('bang
CC sensitivity' = temporarily paralysis in response to a physical jolt).
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB63528.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; M19494; AAA28935.1; -; mRNA.
DR EMBL; AJ250320; CAB65721.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF45781.1; -; Genomic_DNA.
DR EMBL; AL133505; CAB63528.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL133505; CAB65841.1; -; Genomic_DNA.
DR EMBL; BT015958; AAV36843.1; -; mRNA.
DR PIR; A29622; A29622.
DR RefSeq; NP_001245490.1; NM_001258561.1.
DR RefSeq; NP_525050.1; NM_080311.3.
DR AlphaFoldDB; P10735; -.
DR SMR; P10735; -.
DR BioGRID; 57764; 3.
DR DIP; DIP-22123N; -.
DR IntAct; P10735; 1.
DR STRING; 7227.FBpp0300988; -.
DR PaxDb; P10735; -.
DR PRIDE; P10735; -.
DR DNASU; 31228; -.
DR EnsemblMetazoa; FBtr0070459; FBpp0070443; FBgn0003714.
DR GeneID; 31228; -.
DR KEGG; dme:Dmel_CG7925; -.
DR CTD; 31228; -.
DR FlyBase; FBgn0003714; tko.
DR VEuPathDB; VectorBase:FBgn0003714; -.
DR eggNOG; KOG1750; Eukaryota.
DR GeneTree; ENSGT00550000075103; -.
DR HOGENOM; CLU_104295_3_1_1; -.
DR InParanoid; P10735; -.
DR OMA; ASLAQMH; -.
DR PhylomeDB; P10735; -.
DR Reactome; R-DME-5389840; Mitochondrial translation elongation.
DR Reactome; R-DME-5419276; Mitochondrial translation termination.
DR BioGRID-ORCS; 31228; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31228; -.
DR PRO; PR:P10735; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0003714; Expressed in secondary oocyte and 40 other tissues.
DR ExpressionAtlas; P10735; baseline and differential.
DR Genevisible; P10735; DM.
DR GO; GO:0005763; C:mitochondrial small ribosomal subunit; ISS:UniProtKB.
DR GO; GO:0005840; C:ribosome; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; ISS:UniProtKB.
DR GO; GO:0007619; P:courtship behavior; TAS:FlyBase.
DR GO; GO:0008049; P:male courtship behavior; IMP:FlyBase.
DR GO; GO:0007638; P:mechanosensory behavior; IMP:FlyBase.
DR GO; GO:0032543; P:mitochondrial translation; ISS:UniProtKB.
DR GO; GO:0001666; P:response to hypoxia; IDA:FlyBase.
DR GO; GO:0009612; P:response to mechanical stimulus; IMP:FlyBase.
DR GO; GO:0007605; P:sensory perception of sound; IMP:FlyBase.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd03368; Ribosomal_S12; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR006032; Ribosomal_S12/S23.
DR InterPro; IPR005679; Ribosomal_S12_bac.
DR PANTHER; PTHR11652; PTHR11652; 1.
DR Pfam; PF00164; Ribosom_S12_S23; 1.
DR PRINTS; PR01034; RIBOSOMALS12.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW Transit peptide.
FT TRANSIT 1..30
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 31..140
FT /note="40S ribosomal protein S12, mitochondrial"
FT /id="PRO_0000030605"
FT MUTAGEN 85
FT /note="L->H: In allele tko-25t."
FT /evidence="ECO:0000269|PubMed:16120347"
SQ SEQUENCE 140 AA; 15532 MW; 8187A7312C529F31 CRC64;
MNFLRQSFGI TKQLASQAIQ CSYETAVRGM ASLQQMHRSG PHIKTRPPRQ PLDGKPFAKG
VVLKTLIKKP KKPNSANRKC VLVRLSTGKE MVAYIPGIGH NLQEHNIVLC RVGRLQDVPG
VKLKAVRGVY DLAHVVKKSQ
