RT12_HUMAN
ID RT12_HUMAN Reviewed; 138 AA.
AC O15235; Q53X98;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=28S ribosomal protein S12, mitochondrial;
DE Short=MRP-S12;
DE Short=S12mt;
DE AltName: Full=MT-RPS12;
DE AltName: Full=Mitochondrial small ribosomal subunit protein uS12m {ECO:0000303|PubMed:25838379};
DE Flags: Precursor;
GN Name=MRPS12; Synonyms=RPMS12, RPSM12;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver;
RX PubMed=9434165; DOI=10.1016/s0378-1119(97)00521-0;
RA Shah Z.H., O'Dell K.M., Miller S.C.M., An X., Jacobs H.T.;
RT "Metazoan nuclear genes for mitoribosomal protein S12.";
RL Gene 204:55-62(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9790755; DOI=10.1006/geno.1998.5448;
RA Johnson D.F., Hamon M., Fischel-Ghodsian N.;
RT "Characterization of the human mitochondrial ribosomal S12 gene.";
RL Genomics 52:363-368(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, Colon, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
RN [8] {ECO:0007744|PDB:3J9M}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS), SUBCELLULAR LOCATION,
RP AND SUBUNIT.
RX PubMed=25838379; DOI=10.1126/science.aaa1193;
RA Amunts A., Brown A., Toots J., Scheres S.H., Ramakrishnan V.;
RT "Ribosome. The structure of the human mitochondrial ribosome.";
RL Science 348:95-98(2015).
CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt-
CC SSU). Mature mammalian 55S mitochondrial ribosomes consist of a small
CC (28S) and a large (39S) subunit. The 28S small subunit contains a 12S
CC ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large
CC subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial
CC valine transfer RNA (mt-tRNA(Val)), which plays an integral structural
CC role, and 52 different proteins. {ECO:0000269|PubMed:25838379}.
CC -!- INTERACTION:
CC O15235; P58062: SPINK7; NbExp=3; IntAct=EBI-712205, EBI-1182445;
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:25838379}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC {ECO:0000305}.
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DR EMBL; Y11681; CAA72377.1; -; mRNA.
DR EMBL; AF058761; AAC64704.1; -; Genomic_DNA.
DR EMBL; CR407609; CAG28537.1; -; mRNA.
DR EMBL; CH471126; EAW56849.1; -; Genomic_DNA.
DR EMBL; BC001617; AAH01617.1; -; mRNA.
DR EMBL; BC006424; AAH06424.1; -; mRNA.
DR EMBL; BC007499; AAH07499.1; -; mRNA.
DR CCDS; CCDS12525.1; -.
DR RefSeq; NP_066930.1; NM_021107.1.
DR RefSeq; NP_203526.1; NM_033362.3.
DR RefSeq; NP_203527.1; NM_033363.1.
DR PDB; 3J9M; EM; 3.50 A; AJ=1-138.
DR PDB; 6NU2; EM; 3.90 A; AJ=31-138.
DR PDB; 6NU3; EM; 4.40 A; AJ=1-138.
DR PDB; 6RW4; EM; 2.97 A; J=1-138.
DR PDB; 6RW5; EM; 3.14 A; J=1-138.
DR PDB; 6VLZ; EM; 2.97 A; AJ=1-138.
DR PDB; 6VMI; EM; 2.96 A; AJ=1-138.
DR PDB; 6ZM5; EM; 2.89 A; AJ=1-138.
DR PDB; 6ZM6; EM; 2.59 A; AJ=1-138.
DR PDB; 6ZS9; EM; 4.00 A; AJ=1-138.
DR PDB; 6ZSA; EM; 4.00 A; AJ=1-138.
DR PDB; 6ZSB; EM; 4.50 A; AJ=1-138.
DR PDB; 6ZSC; EM; 3.50 A; AJ=1-138.
DR PDB; 6ZSD; EM; 3.70 A; AJ=1-138.
DR PDB; 6ZSE; EM; 5.00 A; AJ=1-138.
DR PDB; 6ZSG; EM; 4.00 A; AJ=1-138.
DR PDB; 7A5F; EM; 4.40 A; J6=1-138.
DR PDB; 7A5G; EM; 4.33 A; J6=1-138.
DR PDB; 7A5I; EM; 3.70 A; J6=1-138.
DR PDB; 7A5K; EM; 3.70 A; J6=1-138.
DR PDB; 7L08; EM; 3.49 A; AJ=1-138.
DR PDB; 7OG4; EM; 3.80 A; AJ=1-138.
DR PDBsum; 3J9M; -.
DR PDBsum; 6NU2; -.
DR PDBsum; 6NU3; -.
DR PDBsum; 6RW4; -.
DR PDBsum; 6RW5; -.
DR PDBsum; 6VLZ; -.
DR PDBsum; 6VMI; -.
DR PDBsum; 6ZM5; -.
DR PDBsum; 6ZM6; -.
DR PDBsum; 6ZS9; -.
DR PDBsum; 6ZSA; -.
DR PDBsum; 6ZSB; -.
DR PDBsum; 6ZSC; -.
DR PDBsum; 6ZSD; -.
DR PDBsum; 6ZSE; -.
DR PDBsum; 6ZSG; -.
DR PDBsum; 7A5F; -.
DR PDBsum; 7A5G; -.
DR PDBsum; 7A5I; -.
DR PDBsum; 7A5K; -.
DR PDBsum; 7L08; -.
DR PDBsum; 7OG4; -.
DR AlphaFoldDB; O15235; -.
DR SMR; O15235; -.
DR BioGRID; 112098; 262.
DR ComplexPortal; CPX-5225; 28S mitochondrial small ribosomal subunit.
DR CORUM; O15235; -.
DR IntAct; O15235; 41.
DR MINT; O15235; -.
DR STRING; 9606.ENSP00000384952; -.
DR iPTMnet; O15235; -.
DR PhosphoSitePlus; O15235; -.
DR SwissPalm; O15235; -.
DR BioMuta; MRPS12; -.
DR EPD; O15235; -.
DR jPOST; O15235; -.
DR MassIVE; O15235; -.
DR MaxQB; O15235; -.
DR PaxDb; O15235; -.
DR PeptideAtlas; O15235; -.
DR PRIDE; O15235; -.
DR ProteomicsDB; 48529; -.
DR TopDownProteomics; O15235; -.
DR Antibodypedia; 30241; 187 antibodies from 26 providers.
DR DNASU; 6183; -.
DR Ensembl; ENST00000308018.9; ENSP00000308845.3; ENSG00000128626.12.
DR Ensembl; ENST00000402029.3; ENSP00000384579.2; ENSG00000128626.12.
DR Ensembl; ENST00000407800.2; ENSP00000384952.1; ENSG00000128626.12.
DR Ensembl; ENST00000634246.1; ENSP00000488926.1; ENSG00000283018.1.
DR Ensembl; ENST00000634400.1; ENSP00000489467.1; ENSG00000283018.1.
DR Ensembl; ENST00000634704.1; ENSP00000489374.1; ENSG00000283018.1.
DR GeneID; 6183; -.
DR KEGG; hsa:6183; -.
DR MANE-Select; ENST00000308018.9; ENSP00000308845.3; NM_033362.4; NP_203526.1.
DR UCSC; uc002okc.4; human.
DR CTD; 6183; -.
DR GeneCards; MRPS12; -.
DR HGNC; HGNC:10380; MRPS12.
DR HPA; ENSG00000128626; Low tissue specificity.
DR MIM; 603021; gene.
DR neXtProt; NX_O15235; -.
DR OpenTargets; ENSG00000128626; -.
DR PharmGKB; PA30996; -.
DR VEuPathDB; HostDB:ENSG00000128626; -.
DR eggNOG; KOG1750; Eukaryota.
DR GeneTree; ENSGT00550000075103; -.
DR HOGENOM; CLU_104295_3_1_1; -.
DR InParanoid; O15235; -.
DR OMA; ASLAQMH; -.
DR OrthoDB; 1542634at2759; -.
DR PhylomeDB; O15235; -.
DR TreeFam; TF315095; -.
DR PathwayCommons; O15235; -.
DR Reactome; R-HSA-5368286; Mitochondrial translation initiation.
DR Reactome; R-HSA-5389840; Mitochondrial translation elongation.
DR Reactome; R-HSA-5419276; Mitochondrial translation termination.
DR SignaLink; O15235; -.
DR SIGNOR; O15235; -.
DR BioGRID-ORCS; 6183; 410 hits in 1086 CRISPR screens.
DR ChiTaRS; MRPS12; human.
DR GeneWiki; MRPS12; -.
DR GenomeRNAi; 6183; -.
DR Pharos; O15235; Tbio.
DR PRO; PR:O15235; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; O15235; protein.
DR Bgee; ENSG00000128626; Expressed in apex of heart and 97 other tissues.
DR ExpressionAtlas; O15235; baseline and differential.
DR Genevisible; O15235; HS.
DR GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
DR GO; GO:0005761; C:mitochondrial ribosome; TAS:ProtInc.
DR GO; GO:0005763; C:mitochondrial small ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0005840; C:ribosome; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0003735; F:structural constituent of ribosome; ISS:UniProtKB.
DR GO; GO:0032543; P:mitochondrial translation; ISS:UniProtKB.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd03368; Ribosomal_S12; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR006032; Ribosomal_S12/S23.
DR InterPro; IPR005679; Ribosomal_S12_bac.
DR PANTHER; PTHR11652; PTHR11652; 1.
DR Pfam; PF00164; Ribosom_S12_S23; 1.
DR PRINTS; PR01034; RIBOSOMALS12.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Mitochondrion; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; Transit peptide.
FT TRANSIT 1..29
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 30..138
FT /note="28S ribosomal protein S12, mitochondrial"
FT /id="PRO_0000030606"
FT REGION 36..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 8
FT /note="H -> R (in dbSNP:rs33988199)"
FT /id="VAR_051820"
SQ SEQUENCE 138 AA; 15173 MW; 893FD2CA1C7187A7 CRC64;
MSWSGLLHGL NTSLTCGPAL VPRLWATCSM ATLNQMHRLG PPKRPPRKLG PTEGRPQLKG
VVLCTFTRKP KKPNSANRKC CRVRLSTGRE AVCFIPGEGH TLQEHQIVLV EGGRTQDLPG
VKLTVVRGKY DCGHVQKK
