ID   BCN5_CLOPF              Reviewed;         890 AA.
AC   P08696;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Bacteriocin BCN5;
GN   Name=bcn;
OS   Clostridium perfringens.
OG   Plasmid pIP404.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1502;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CPN50;
RX   PubMed=2901768; DOI=10.1016/0147-619x(88)90052-2;
RA   Garnier T., Cole S.T.;
RT   "Complete nucleotide sequence and genetic organization of the
RT   bacteriocinogenic plasmid, pIP404, from Clostridium perfringens.";
RL   Plasmid 19:134-150(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CPN50;
RX   PubMed=2877971; DOI=10.1128/jb.168.3.1189-1196.1986;
RA   Garnier T., Cole S.T.;
RT   "Characterization of a bacteriocinogenic plasmid from Clostridium
RT   perfringens and molecular genetic analysis of the bacteriocin-encoding
RT   gene.";
RL   J. Bacteriol. 168:1189-1196(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
RC   STRAIN=CPN50;
RX   PubMed=2460717; DOI=10.1111/j.1365-2958.1988.tb00069.x;
RA   Garnier T., Cole S.T.;
RT   "Studies of UV-inducible promoters from Clostridium perfringens in vivo and
RT   in vitro.";
RL   Mol. Microbiol. 2:607-614(1988).
CC   -!- FUNCTION: May function as an ionophore.
CC   -!- INDUCTION: By UV irradiation.
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DR   EMBL; M14481; AAA98248.1; -; Genomic_DNA.
DR   EMBL; M32882; AAA98249.1; -; Genomic_DNA.
DR   PIR; A30481; A30481.
DR   RefSeq; NP_040451.1; NC_001388.1.
DR   RefSeq; WP_010889923.1; NC_001388.1.
DR   AlphaFoldDB; P08696; -.
DR   SMR; P08696; -.
DR   GO; GO:0004181; F:metallocarboxypeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   InterPro; IPR000834; Peptidase_M14.
DR   InterPro; IPR003646; SH3-like_bac-type.
DR   Pfam; PF00246; Peptidase_M14; 1.
DR   Pfam; PF08239; SH3_3; 1.
DR   SMART; SM00287; SH3b; 3.
DR   PROSITE; PS51781; SH3B; 3.
PE   2: Evidence at transcript level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Plasmid.
FT   CHAIN           1..890
FT                   /note="Bacteriocin BCN5"
FT                   /id="PRO_0000110576"
FT   DOMAIN          22..84
FT                   /note="SH3b 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01117"
FT   DOMAIN          179..241
FT                   /note="SH3b 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01117"
FT   DOMAIN          572..636
FT                   /note="SH3b 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01117"
FT   REGION          815..869
FT                   /note="Hydrophobic"
SQ   SEQUENCE   890 AA;  96700 MW;  F4E5E8971C31C6C6 CRC64;
     MANNIIPNVS SGDLVGSTPT FPPNAVVRGD FLYLRDVDGN QIPGRTVSDG DEITVLFISN
     EKNIVLVQYP TSSGYRQGYV TNATSIIKYK DDYSWVNGST PEPVYDFDKT TQIGTLDPRE
     RAVVLYKVDG MTYVAYDTGK GKLTKSGLVH YEGSGSSTGG GSFNGVAPGE VVPGGFTYEN
     NAEVVGDELY LRDANGNLIP GRSVSVGDKI TVLDVGYTKQ LALVQYPAGD VVRQGYVTNA
     TNLIRYFNQY SWHNGSTSEE VLDENGGHLG SLNPYEAATL LYEKNGMKHV VYDTNKGPNT
     KSGYVKYEGA AATRVDIPYP SITNAQKIVY GISGRGRELA AYKVGNGSNS LVFVCAIHGW
     EDNWAADGIE LTRIGNGLIE HFQNAGTNNW SLYIIPVANP DGLSEGFTNN GPGRCTIVGA
     VDCNRDFPLG FSPGGVPRYH SGSEPLSVSE SKSLHDFIQG VKNRTSGEMC VIDLHGWEGA
     AIGNPEIGEY FRNQFGFGQR SGYGDNRGFM IGWAKSIGAK AALIELPGST KSHSDVVNGR
     YLQKIINAVT NLIGGSGGSS SGGSSFSDVS YEATGEVINV QSFLNVREGA GLYTNSIGQL
     RQGNKVNIVA KNGDWYKIKY GSEYGYVNSG YIIILKNNTS VKLEDWQEDC IKFGWGPITK
     EKYLEYMDST RLYKSIENDI SQAIKNKSLI NVINPLNFSV SEMIACTQIV FNNETTSFFR
     DEWYSKSNPN FIVKYKKLSN GQIIVLDRIN IKKPEKLKTK IPKAAKGAFK DTIKFEFFKG
     IDGWFTAISG AISIGSDLSV FQSNGELKSN EDIAKALAAA VIVNGVETMF CAFLGGFIAQ
     CIAPEFPIVA AVAGAIVSAI AAFAIGYFVD NHEKEKYLMN SFKGLIDYLF