RT15_DROME
ID RT15_DROME Reviewed; 280 AA.
AC Q8WTC1; Q9W231;
DT 30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2003, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=28S ribosomal protein S15, mitochondrial;
DE Short=MRP-S15;
DE Short=S15mt;
DE AltName: Full=DmMRPS15;
DE AltName: Full=Protein bonsai;
DE Flags: Precursor;
GN Name=bonsai; Synonyms=mRpS15; ORFNames=CG4207;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DISRUPTION PHENOTYPE.
RC TISSUE=Embryo;
RX PubMed=14651932; DOI=10.1016/j.ydbio.2003.08.021;
RA Galloni M.;
RT "Bonsai, a ribosomal protein S15 homolog, involved in gut mitochondrial
RT activity and systemic growth.";
RL Dev. Biol. 264:482-494(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10225996; DOI=10.1242/dev.126.11.2365;
RA Galloni M., Edgar B.A.;
RT "Cell-autonomous and non-autonomous growth-defective mutants of Drosophila
RT melanogaster.";
RL Development 126:2365-2375(1999).
CC -!- FUNCTION: Essential for gut mitochondrial activity. Might be involved
CC in tissue specific growth factor production.
CC {ECO:0000269|PubMed:10225996, ECO:0000269|PubMed:14651932}.
CC -!- SUBUNIT: Component of the mitochondrial ribosome small subunit (28S)
CC which comprises a 12S rRNA and about 30 distinct proteins.
CC {ECO:0000250|UniProtKB:P82913}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14651932}.
CC -!- TISSUE SPECIFICITY: Expressed in anterior and posterior midgut
CC primordia in stage 11 embryos. In stage 13 embryos, expression is high
CC in the developing midgut and hindgut. In stage 16 embryos, expression
CC is elevated in the midgut, hindgut, and in a small region that will
CC give rise to pharyngeal muscles and to the stomatogastric nervous
CC system. In larvae, expression is predominant in the gut, and head,
CC presumably in pharyngeal muscles. {ECO:0000269|PubMed:10225996,
CC ECO:0000269|PubMed:14651932}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically
CC throughout development, lowest levels are in third larval instar and
CC pupae.
CC -!- DISRUPTION PHENOTYPE: Mutants exhibit a strong systemic growth defect
CC and die before reaching wild-type size. {ECO:0000269|PubMed:14651932}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS15 family.
CC {ECO:0000305}.
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DR EMBL; AF435962; AAL31972.1; -; mRNA.
DR EMBL; AE013599; AAF46869.1; -; Genomic_DNA.
DR EMBL; AY060712; AAL28260.1; -; mRNA.
DR RefSeq; NP_611691.1; NM_137847.3.
DR AlphaFoldDB; Q8WTC1; -.
DR SMR; Q8WTC1; -.
DR BioGRID; 63202; 3.
DR IntAct; Q8WTC1; 1.
DR STRING; 7227.FBpp0071771; -.
DR PaxDb; Q8WTC1; -.
DR PRIDE; Q8WTC1; -.
DR DNASU; 37587; -.
DR EnsemblMetazoa; FBtr0071860; FBpp0071771; FBgn0026261.
DR GeneID; 37587; -.
DR KEGG; dme:Dmel_CG4207; -.
DR UCSC; CG4207-RA; d. melanogaster.
DR CTD; 37587; -.
DR FlyBase; FBgn0026261; bonsai.
DR VEuPathDB; VectorBase:FBgn0026261; -.
DR eggNOG; KOG2815; Eukaryota.
DR GeneTree; ENSGT00390000001737; -.
DR HOGENOM; CLU_1009058_0_0_1; -.
DR InParanoid; Q8WTC1; -.
DR OMA; QEQISCD; -.
DR OrthoDB; 1539741at2759; -.
DR PhylomeDB; Q8WTC1; -.
DR Reactome; R-DME-5389840; Mitochondrial translation elongation.
DR Reactome; R-DME-5419276; Mitochondrial translation termination.
DR BioGRID-ORCS; 37587; 0 hits in 1 CRISPR screen.
DR ChiTaRS; bonsai; fly.
DR GenomeRNAi; 37587; -.
DR PRO; PR:Q8WTC1; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0026261; Expressed in egg chamber and 35 other tissues.
DR Genevisible; Q8WTC1; DM.
DR GO; GO:0005763; C:mitochondrial small ribosomal subunit; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0003735; F:structural constituent of ribosome; ISS:UniProtKB.
DR GO; GO:0032543; P:mitochondrial translation; ISS:UniProtKB.
DR GO; GO:0040008; P:regulation of growth; IMP:UniProtKB.
DR CDD; cd00353; Ribosomal_S15p_S13e; 1.
DR HAMAP; MF_01343_B; Ribosomal_S15_B; 1.
DR InterPro; IPR000589; Ribosomal_S15.
DR InterPro; IPR005290; Ribosomal_S15_bac-type.
DR InterPro; IPR009068; S15_NS1_RNA-bd.
DR Pfam; PF00312; Ribosomal_S15; 1.
DR SMART; SM01387; Ribosomal_S15; 1.
DR SUPFAM; SSF47060; SSF47060; 1.
PE 2: Evidence at transcript level;
KW Mitochondrion; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..280
FT /note="28S ribosomal protein S15, mitochondrial"
FT /id="PRO_0000030617"
FT CONFLICT 30
FT /note="M -> V (in Ref. 1; AAL31972)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="A -> S (in Ref. 1; AAL31972)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 280 AA; 33473 MW; 4CB921573734A08B CRC64;
MNKLLNIAQA GHRQFVREYA FKSDLKIKWM RPEKIACYKP EKSGDLAKLP PLKADELLPE
YRDCKELDKA DESVKSLFKL SNNASYLTTK FYRDEMVKEV QRHAQDFGSM EAKLAKMTAV
IRRYQEHMDK HPRDKMIKVR LKELIDKRKK FLKYLRRWDY PRFEWILEKL DLVYKPPPTH
FHWITRKESL QKLTDIYCEN LKEERLEAYH KQLQAQQIPF LEEAIKKMQF VRQEQISCDV
PVTVTEEKIA DSKRQLEMLK ELQQAEAAAS SKKQNEDGFN
