RT18B_MOUSE
ID RT18B_MOUSE Reviewed; 254 AA.
AC Q99N84; Q3TLA9; Q9CRK0; Q9DCR8;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=28S ribosomal protein S18b, mitochondrial;
DE Short=MRP-S18-b;
DE Short=Mrps18-b;
DE Short=S18mt-b;
DE AltName: Full=28S ribosomal protein S18-2, mitochondrial;
DE Short=MRP-S18-2;
DE Flags: Precursor;
GN Name=Mrps18b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=11402041; DOI=10.1074/jbc.m103236200;
RA Suzuki T., Terasaki M., Takemoto-Hori C., Hanada T., Ueda T., Wada A.,
RA Watanabe K.;
RT "Proteomic analysis of the mammalian mitochondrial ribosome. Identification
RT of protein components in the 28S small subunit.";
RL J. Biol. Chem. 276:33181-33195(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Embryonic stem cell, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Component of the mitochondrial ribosome small subunit (28S)
CC which comprises a 12S rRNA and about 30 distinct proteins.
CC {ECO:0000250|UniProtKB:Q9Y676}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q9Y676}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q99N84-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99N84-2; Sequence=VSP_005723;
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bS18 family.
CC Mitochondrion-specific ribosomal protein mS40 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK010250; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB049954; BAB41007.1; -; mRNA.
DR EMBL; AK002554; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK010250; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK166597; BAE38883.1; -; mRNA.
DR EMBL; BC021752; AAH21752.1; -; mRNA.
DR CCDS; CCDS28712.1; -. [Q99N84-1]
DR CCDS; CCDS84307.1; -. [Q99N84-2]
DR RefSeq; NP_080154.1; NM_025878.1. [Q99N84-1]
DR AlphaFoldDB; Q99N84; -.
DR SMR; Q99N84; -.
DR BioGRID; 211847; 5.
DR ComplexPortal; CPX-5301; 28S mitochondrial small ribosomal subunit.
DR STRING; 10090.ENSMUSP00000025305; -.
DR iPTMnet; Q99N84; -.
DR PhosphoSitePlus; Q99N84; -.
DR SwissPalm; Q99N84; -.
DR EPD; Q99N84; -.
DR MaxQB; Q99N84; -.
DR PaxDb; Q99N84; -.
DR PeptideAtlas; Q99N84; -.
DR PRIDE; Q99N84; -.
DR ProteomicsDB; 260940; -. [Q99N84-1]
DR ProteomicsDB; 260941; -. [Q99N84-2]
DR Antibodypedia; 26399; 85 antibodies from 21 providers.
DR DNASU; 66973; -.
DR Ensembl; ENSMUST00000025305; ENSMUSP00000025305; ENSMUSG00000024436. [Q99N84-1]
DR Ensembl; ENSMUST00000113782; ENSMUSP00000109412; ENSMUSG00000024436. [Q99N84-2]
DR GeneID; 66973; -.
DR KEGG; mmu:66973; -.
DR UCSC; uc008cjg.1; mouse. [Q99N84-1]
DR CTD; 28973; -.
DR MGI; MGI:1914223; Mrps18b.
DR VEuPathDB; HostDB:ENSMUSG00000024436; -.
DR eggNOG; KOG4021; Eukaryota.
DR GeneTree; ENSGT00390000010554; -.
DR HOGENOM; CLU_138628_0_0_1; -.
DR InParanoid; Q99N84; -.
DR OMA; PWEYLET; -.
DR OrthoDB; 1023024at2759; -.
DR PhylomeDB; Q99N84; -.
DR TreeFam; TF315059; -.
DR Reactome; R-MMU-5389840; Mitochondrial translation elongation.
DR Reactome; R-MMU-5419276; Mitochondrial translation termination.
DR BioGRID-ORCS; 66973; 19 hits in 72 CRISPR screens.
DR ChiTaRS; Mrps18b; mouse.
DR PRO; PR:Q99N84; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q99N84; protein.
DR Bgee; ENSMUSG00000024436; Expressed in morula and 68 other tissues.
DR ExpressionAtlas; Q99N84; baseline and differential.
DR Genevisible; Q99N84; MM.
DR GO; GO:0030054; C:cell junction; ISO:MGI.
DR GO; GO:0005743; C:mitochondrial inner membrane; IC:ComplexPortal.
DR GO; GO:0005763; C:mitochondrial small ribosomal subunit; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0003735; F:structural constituent of ribosome; ISS:UniProtKB.
DR GO; GO:0032543; P:mitochondrial translation; ISS:UniProtKB.
DR Gene3D; 4.10.640.10; -; 1.
DR InterPro; IPR040054; MRPS18B.
DR InterPro; IPR001648; Ribosomal_S18.
DR InterPro; IPR036870; Ribosomal_S18_sf.
DR PANTHER; PTHR13329; PTHR13329; 1.
DR Pfam; PF01084; Ribosomal_S18; 1.
DR SUPFAM; SSF46911; SSF46911; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Mitochondrion; Phosphoprotein; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; Transit peptide.
FT TRANSIT 1..33
FT /note="Mitochondrion"
FT /evidence="ECO:0000250|UniProtKB:P82918"
FT CHAIN 34..254
FT /note="28S ribosomal protein S18b, mitochondrial"
FT /id="PRO_0000030628"
FT REGION 218..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 238..254
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 47
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y676"
FT VAR_SEQ 25..116
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_005723"
SQ SEQUENCE 254 AA; 28703 MW; 2284983FD5848791 CRC64;
MAAPLRHTLL KLVPTLLRSS YVAQVPLQTL CTRGPPEEDA PSSLPVSPYE SEPWKYLDSE
EYHNRYGSRP VWADYRRNHK GGVPPQRTRK TCIRNNKVAG NPCPICRDHK LHVDFRNVKL
LEQFVCAHTG IIFHAPYTGV CMKQHKKLTQ AIQKARECGL LSYYVPQVEP RDADFGTVHG
AVSVTPPAPT LLSGEPWYPW YSWQQPPERE LSRLRRLYQG NLLEESGPPP ESMPEMPTTP
PAESSIEQPG SQSA
